Amino acid sequence of the Homo sapiens brain 21-23-kDa protein (Neuropolypeptide h3), comparison with its counterparts from Rattus norvegicus and Bos taurus species, and expression of its mRNA in different tissues

1994 ◽  
Vol 39 (6) ◽  
pp. 655-660 ◽  
Author(s):  
Nadia Seddiqi ◽  
Francine Bollengier ◽  
Patrick M. Alliel ◽  
Jean-Pierre P�rin ◽  
Fran�ois Bonnet ◽  
...  
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Rattus Norvegicus ◽  
Bos Taurus ◽  
Homo Sapiens ◽  
Different Tissues

scholarly journals The amino acid sequence of the α chain of the major haemoglobin of the rat (Rattus norvegicus)

1975 ◽  
Vol 149 (1) ◽  
pp. 259-269 ◽  
Author(s):  
C G Chua ◽  
R W Carrell ◽  
B H Howard
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Rattus Norvegicus ◽  
Peptide Mapping ◽  
Cysteine Residue ◽  
British Library ◽  
Peptide Fragments ◽  
Partial Amino Acid Sequence ◽  
Tryptic Peptides ◽  
Α Chain

1. A partial amino acid sequence of the α chain from the rat (Wistar, Rattus norvegicus) major haemoglobin is reported. The soluble tryptic peptides prepared from aminoethylated α-globin were separated by peptide ‘mapping’. Sequencing of the tryptic peptides was carried out by the dansyl-Edman method and by the overlapping of smaller peptide fragments derived from secondary enzymic digestion. The insoluble ‘core’ peptides were further digested with chymotrypsin, thermolysin and pepsin to give smaller soluble peptides for sequencing. The tryptic peptides were ordered on the basis of their homology with the corresponding peptides of human α chain. 2. The proposed sequence is compared with that obtained by using an automated sequencer [Garrick et al. (1975) Biochem. J.149, 245-258]. The differences in sequence resulting from the two methods are discussed. 3. It is suggested that the externally situated cysteine (residue 13) is responsible for the observed inhibition of crystallization of rat haemoglobin at alkaline pH. 4. Detailed evidence for the sequence has been deposited as Supplementary Publication SUP 50047 (9 pages) at the British Library (Linding Division), Boston Spa, Wetherby, West Yorkshire LS23 7BQ, U.K., from which copies can be obtained on the terms given in Biochem. J. (1975) 145, 5.

Cloning and expression of caprine KIT gene and associations of polymorphisms with litter size

2016 ◽  
Vol 56 (10) ◽  
pp. 1579 ◽  
Author(s):  
X. P. An ◽  
J. X. Hou ◽  
T. Y. Gao ◽  
B. Y. Cao
Keyword(s):  
Amino Acid ◽  
Litter Size ◽  
Bos Taurus ◽  
Homo Sapiens ◽  
Ovis Aries ◽  
Cloning And Expression ◽  
Nucleotide Polymorphisms ◽  
Coding Region ◽  
Reading Frame ◽  
Kit Gene

The full coding region of KIT mRNA was cloned from the caprine ovary. The results showed the caprine KIT cDNA (GenBank accession number KF364483) contained a 2925-bp open reading frame encoding a protein with 974 amino acid residues. BLAST analysis revealed that the caprine KIT protein had high similarity with that of four species: Ovis aries (99%), Bos taurus (99%), Sus scrofa (94%) and Homo sapiens (90%). The KIT mRNA expression pattern showed that KIT mRNA was expressed highly in kidney, ovary, uterus and breast. Two single nucleotide polymorphisms (g.88430T > A and g.120466G > A) in the caprine KIT gene were detected by PCR–restriction fragment length polymorphism (RFLP) and DNA sequencing in 735 goats of Xinong Saanen, Guanzhong and Boer breeds. The g.88430T > A mutation was a missense mutation (Tyr > Asn at position 409 amino acid of KIT). The association study has been done by jointly analysing all data in one analysis. The result showed that individuals with TT and TA genotypes had their litter size increased by 0.11 and 0.09, respectively, compared with those with AA genotype at the g.88430T > A locus for three goat breeds (P < 0.05). Further analysis revealed that combined genotype TTAA was better than the others for litter size in three goat breeds. Therefore, the biochemical and physiological functions, together with the results obtained in our investigation, suggest that the KIT gene could serve as a genetic marker for litter size in goat breeding.

scholarly journals Structure of the CLC-1 chloride channel from Homo sapiens

eLife ◽  
2018 ◽  
Vol 7 ◽  
Author(s):  
Eunyong Park ◽  
Roderick MacKinnon
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Proton Transfer ◽  
Opposite Direction ◽  
Chloride Channel ◽  
Pore Diameter ◽  
Homo Sapiens ◽  
Transport Function ◽  
Key Sites ◽  
Internal Pore

CLC channels mediate passive Cl− conduction, while CLC transporters mediate active Cl− transport coupled to H+ transport in the opposite direction. The distinction between CLC-0/1/2 channels and CLC transporters seems undetectable by amino acid sequence. To understand why they are different functionally we determined the structure of the human CLC-1 channel. Its ‘glutamate gate’ residue, known to mediate proton transfer in CLC transporters, adopts a location in the structure that appears to preclude it from its transport function. Furthermore, smaller side chains produce a wider pore near the intracellular surface, potentially reducing a kinetic barrier for Cl− conduction. When the corresponding residues are mutated in a transporter, it is converted to a channel. Finally, Cl− at key sites in the pore appear to interact with reduced affinity compared to transporters. Thus, subtle differences in glutamate gate conformation, internal pore diameter and Cl− affinity distinguish CLC channels and transporters.

scholarly journals Association of the Ile-442-Met substitution in <i>NCAPG</i> with birth weight in German Angus and German Simmental cattle (Brief Report)

2011 ◽  
Vol 54 (1) ◽  
pp. 104-106
Author(s):  
K. Glenske ◽  
H. Brandt ◽  
G. Erhardt
Keyword(s):  
Birth Weight ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Beef Cattle ◽  
Genetic Background ◽  
Bos Taurus ◽  
Cattle Population ◽  
Commercial Line ◽  
Beef Cattle Breeds ◽  
Simmental Cattle

Abstract. The optimal birth weight (BW) of a calf is important and an indicator of the chance to survive and performing later in life (Maltecca et al. 2009). The search for the genetic background of BW in cattle on BTA6 is in the focus of many studies. One QTL at 1 cM was described in an Angus × Brahman cross population (Kim et al. 2003). Three QTL for BW had been identified at BTA6 at 10 cM, 35-49 cM and 83-86 cM in a commercial line of Bos taurus (Kneeland et al. 2004). One of these was confirmed in a Jersey × Holstein cross population as a putative QTL at 41 cM (Maltecca et al. 2009). In a Holstein × Charolais cross cattle population a highly significant QTL for BW was detected on BTA6 in the same region between BM1329 and DIK1054 (Gutierrez-GIL et al. 2009). In another study with Holstein × Charolais the QTL was confirmed and the SNP in this QTL with the strongest effect on BW was in non-SMC condensing I complex, subunit G (NCAPG 1326T/G) (Eberlein et al. 2009). This SNP induced a Ile-442-Met substitution in the amino acid sequence. It was the aim of this study to validate this SNP by analysing the effect of the Ile-442-Met substitution in NCAPG on birth weight in two important beef cattle breeds in Germany.

scholarly journals Evolution of the Angiopoietin-Like Gene Family and Their Role in Lipid Metabolism in Pigs

2021 ◽  
Author(s):  
Zibin Zheng ◽  
Wentao Lyu ◽  
Qihua Hong ◽  
Hua Yang ◽  
Ying Li ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Lipid Metabolism ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Subcutaneous Fat ◽  
Family Members ◽  
Carcass Quality ◽  
Amino Acid Sequence Analysis ◽  
Different Tissues

Abstract Background: Lipid metabolism is closely associated with various metabolic diseases, such as obesity, cardiovascular disease, diabetes, and cancer. More importantly, it also affects the carcass quality of animals. Angiopoietin-like protein (Angiopoietin-like, ANGPTL) family members are encoded by 8 genes and play an important role in lipid metabolism and angiogenesis. In this study, we first systematically described the phylogenetic characteristics of pig ANGPTL family genes and identified the critical roles of ANGPTL3, ANGPTL4 and ANGPTL8 in the lipid metabolism of pigs.Methods: The amino acid sequence analysis, phylogenetic analysis, and chromosome adjacent gene analysis were performed to identify the ANGPTL gene family in pigs. Furthermore, according to the body weight data from 60 Jinhua pigs, different tissues of 6 pigs with average body weight were used to determine the expression profile of ANGPTL1-8. The ileum, subcutaneous fat, and liver of 8 pigs with distinct fatness were selected to analyze the gene expression of ANGPTL3, ANGPTL4, and ANGPTL8. Results: Results showed the sequence length of ANGPTLs in pigs was between 1186 and 1991 bp, and the pig ANGPTL family members shared common features with human homologous genes, including the high similarity of the amino acid sequence and chromosome flanking genes. Amino acid sequence analysis showed that ANGPTL1-7 had a highly conserved domain except for ANGPTL8. Phylogenetic analysis showed that the ANGPTL homologues identified from 6 species could be divided into two categories. Each ANGPTL homologous gene was clustered with other mammalian sequences, away from other vertebrates. RT-qPCR analysis showed that ANGPTL family members had different expression patterns in different tissues. ANGPTL3 and ANGPTL8 were mainly expressed in the liver, while ANGPTL4 was expressed in many other tissues, such as the intestine and subcutaneous fat. The expression levels of ANGPTL3 and ANGPTL4 in Jinhua pigs with low propensity for adipogenesis were significantly higher than those of high propensity for adipogenesis. Conclusions: These results increase our knowledge about the biological role of the ANGPTL family in this important economic species. In addition, it will also help to better understand the role of ANGPTL3, ANGPTL4 and ANGPTL8 in lipid metabolism of pigs, and provide innovative ideas for develop strategies to improve pig carcass quality.

scholarly journals Evolution of The Angiopoietin-Like Gene Family and Their Role in Lipid Metabolism in Pigs

2021 ◽  
Author(s):  
Zibin Zheng ◽  
Wentao Lyu ◽  
Qihua Hong ◽  
Hua Yang ◽  
Ying Li ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Lipid Metabolism ◽  
Amino Acid ◽  
Amino Acid Sequence ◽  
Subcutaneous Fat ◽  
Family Members ◽  
Carcass Quality ◽  
Amino Acid Sequence Analysis ◽  
Different Tissues

Abstract Background Lipid metabolism is closely associated with various metabolic diseases, such as obesity, cardiovascular disease, diabetes, and cancer. More importantly, it also affects the carcass quality of animals. Angiopoietin-like protein (Angiopoietin-like, ANGPTL) family members are encoded by 8 genes and play an important role in lipid metabolism and angiogenesis. In this study, we first systematically described the phylogenetic characteristics of pig ANGPTL family genes and identified the critical roles of ANGPTL3, ANGPTL4 and ANGPTL8 in the lipid metabolism of pigs. Methods The amino acid sequence analysis, phylogenetic analysis, and chromosome adjacent gene analysis were performed to identify the ANGPTL gene family in pigs. Furthermore, according to the body weight data from 60 Jinhua pigs, different tissues of 6 pigs with average body weight were used to determine the expression profile of ANGPTL1-8. The ileum, subcutaneous fat, and liver of 8 pigs with distinct fatness were selected to analyze the gene expression of ANGPTL3, ANGPTL4, and ANGPTL8. Results Results showed the sequence length of ANGPTLs in pigs was between 1186 and 1991 bp, and the pig ANGPTL family members shared common features with human homologous genes, including the high similarity of the amino acid sequence and chromosome flanking genes. Amino acid sequence analysis showed that ANGPTL1-7 had a highly conserved domain except for ANGPTL8. Phylogenetic analysis showed that each ANGPTL homologous gene was clustered with other mammalian sequences, away from other vertebrates. RT-qPCR analysis showed that ANGPTL family members had different expression patterns in different tissues. ANGPTL3 and ANGPTL8 were mainly expressed in the liver, while ANGPTL4 was expressed in many other tissues, such as the intestine and subcutaneous fat. The expression levels of ANGPTL3 and ANGPTL4 in Jinhua pigs with low propensity for adipogenesis were significantly higher than those of high propensity for adipogenesis. Conclusions These results increase our knowledge about the biological role of the ANGPTL family in this important economic species. In addition, it will also help to better understand the role of ANGPTL3, ANGPTL4 and ANGPTL8 in lipid metabolism of pigs, and provide innovative ideas for develop strategies to improve pig carcass quality.

The staining pattern of the tropomyosin sequence is displayed in a new paracrystal

1986 ◽  
Vol 44 ◽  
pp. 150-151
Author(s):  
M.K. Lamvik ◽  
L.L. Klatt
Keyword(s):  
Amino Acid ◽  
Amino Acid Sequence ◽  
Staining Pattern ◽  
Banding Patterns ◽  
Mass Thickness ◽  
New Form

Tropomyosin paracrystals have been used extensively as test specimens and magnification standards due to their clear periodic banding patterns. The paracrystal type discovered by Ohtsuki1 has been of particular interest as a test of unstained specimens because of alternating bands that differ by 50% in mass thickness. While producing specimens of this type, we came across a new paracrystal form. Since this new form displays aligned tropomyosin molecules without the overlaps that are characteristic of the Ohtsuki-type paracrystal, it presents a staining pattern that corresponds to the amino acid sequence of the molecule.

Sign in / Sign up

Export Citation Format

Share Document