Gradient of anthocyanin in cell aggregates of Daucus carota in suspension cultures

1996 ◽  
Vol 18 (11) ◽  
pp. 1253-1256 ◽  
Author(s):  
R. Madhusudhan ◽  
G. A. Ravishankar
Keyword(s):  
Daucus Carota ◽  
Suspension Cultures ◽  
Cell Aggregates

Preservation of suspension cultures of plant cells by freezing

1971 ◽  
Vol 49 (7) ◽  
pp. 1253-1254 ◽  
Author(s):  
R. Latta
Keyword(s):  
Daucus Carota ◽  
Plant Cells ◽  
Suspension Cultures ◽  
Protective Agent ◽  
Short Period ◽  
Conventional Medium

Development of a method of preserving suspension cultures of plant cells has been undertaken. When grown in a conventional medium containing 20 g/1 of sucrose, Daucus carota cells survived freezing and a short period of storage at −40 °C or −196 °C in the presence of a suitable protective agent, whereas Ipomoea sp. cells did not. Ipomoea cells survived freezing if they were adapted to growth in a medium containing 65 g/1 of sucrose.

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