Inhibition of flavonoid biosynthesis by gibberellic acid in cell suspension cultures of Daucus carota L.

Planta ◽  
1984 ◽  
Vol 160 (6) ◽  
pp. 544-549 ◽  
Author(s):  
W. Hinderer ◽  
M. Petersen ◽  
H. U. Seitz
Keyword(s):  
Gibberellic Acid ◽  
Cell Suspension ◽  
Daucus Carota ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Flavonoid Biosynthesis ◽  
Daucus Carota L

Randomization of the Flavonoid A Ring during Biosynthesis of Kaempferol from [1,2 -13C2] Acetate in Cell Suspension Cultures of Parsley

1980 ◽  
Vol 35 (9-10) ◽  
pp. 717-721 ◽  
Author(s):  
Robley J. Light ◽  
Klaus Hahlbrock
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Flavonoid Biosynthesis ◽  
Flavonoid Glycosides ◽  
Dilute Acid ◽  
Pyrone Ring ◽  
Glycoside Hydrolysis

Abstract Cell suspension cultures of parsley (Petroselinum hortense) were incubated with [l,2-13C2]ace-tate during a period of active flavonoid production. The flavonoid glycosides were extracted with ethanol, hydrolyzed in dilute acid, and the aglycones purified by chromatography. Apigenin, a flavone, and kaempferol, a flavonol, were analyzed at 67.9 MHz by 13C FT NMR. The 13C enrich­ ment confirmed that acetate contributes primarily to the flavonoid A ring. The coupling patterns between adjacent 13C atoms of the A ring indicate that the cyclization direction of the A ring is random in both compounds. While randomization of apigenin could have occurred chemically through opening of the pyrone ring under the acid conditions used for glycoside hydrolysis, ran­ domization of the more stable flavonol must have occurred biosynthetically. The latter result supports the conclusion that a chalcone is an intermediate in flavonoid biosynthesis.

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