In vitro cytotoxicity of chrysotile asbestos to human pulmonary alveolar macrophages is decreased by organosilane coating and surfactant

1986 ◽  
Vol 2 (2) ◽  
pp. 293-309 ◽  
Author(s):  
David G. Morrison ◽  
Theodore L. McLemore ◽  
E. Clinton Lawrence ◽  
David G. Feuerbacher ◽  
Myles L. Mace ◽  
...  
Keyword(s):  
Alveolar Macrophages ◽  
In Vitro Cytotoxicity ◽  
Chrysotile Asbestos ◽  
Pulmonary Alveolar Macrophages

scholarly journals LPS-induced expression of CD14 in the TRIF pathway is epigenetically regulated by sulforaphane in porcine pulmonary alveolar macrophages

2016 ◽  
Vol 22 (8) ◽  
pp. 682-695 ◽  
Author(s):  
Qin Yang ◽  
Maren J Pröll ◽  
Dessie Salilew-Wondim ◽  
Rui Zhang ◽  
Dawit Tesfaye ◽  
...  
Keyword(s):  
Gene Expression ◽  
Alveolar Macrophages ◽  
Methylation Status ◽  
Gene Body ◽  
Gene Body Methylation ◽  
Tnf Α ◽  
Pulmonary Alveolar Macrophages ◽  
Cluster Of Differentiation ◽  
Cd14 Gene

Pulmonary alveolar macrophages (AMs) are important in defense against bacterial lung inflammation. Cluster of differentiation 14 (CD14) is involved in recognizing bacterial lipopolysaccharide (LPS) through MyD88-dependent and TRIF pathways of innate immunity. Sulforaphane (SFN) shows anti-inflammatory activity and suppresses DNA methylation. To identify CD14 epigenetic changes by SFN in the LPS-induced TRIF pathway, an AMs model was investigated in vitro. CD14 gene expression was induced by 5 µg/ml LPS at the time point of 12 h and suppressed by 5 µM SFN. After 12 h of LPS stimulation, gene expression was significantly up-regulated, including TRIF, TRAF6, NF-κB, TRAF3, IRF7, TNF-α, IL-1β, IL-6, and IFN-β. LPS-induced TRAM, TRIF, RIPK1, TRAF3, TNF-α, IL-1β and IFN-β were suppressed by 5 µM SFN. Similarly, DNMT3a expression was increased by LPS but significantly down-regulated by 5 µM SFN. It showed positive correlation of CD14 gene body methylation with in LPS-stimulated AMs, and this methylation status was inhibited by SFN. This study suggests that SFN suppresses CD14 activation in bacterial inflammation through epigenetic regulation of CD14 gene body methylation associated with DNMT3a. The results provide insights into SFN-mediated epigenetic down-regulation of CD14 in LPS-induced TRIF pathway inflammation and may lead to new methods for controlling LPS-induced inflammation in pigs.

Growth of Pulmonary Alveolar Macrophages in vitro

Nature ◽  
1973 ◽  
Vol 245 (5421) ◽  
pp. 150-152 ◽  
Author(s):  
S. C. SODERLAND ◽  
Y. NAUM
Keyword(s):  
Alveolar Macrophages ◽  
Pulmonary Alveolar Macrophages

Glutathione release by pulmonary alveolar macrophages in response to particles in vitro

1992 ◽  
Vol 60 (1) ◽  
pp. 53-60 ◽  
Author(s):  
Dea S. Boehme ◽  
Kirk R. Maples ◽  
Rogene F. Henderson
Keyword(s):  
Alveolar Macrophages ◽  
Pulmonary Alveolar Macrophages

scholarly journals Effects of oxygen exposure on in vitro function of pulmonary alveolar macrophages.

1975 ◽  
Vol 56 (2) ◽  
pp. 503-511 ◽  
Author(s):  
S A Murphey ◽  
J S Hyams ◽  
A B Fisher ◽  
R K Root
Keyword(s):  
Alveolar Macrophages ◽  
Oxygen Exposure ◽  
Pulmonary Alveolar Macrophages
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