Efficient plant regeneration from protoplasts of Arachis paraguariensis Chod. et Hassl. using a nurse culture method

1993 ◽  
Vol 34 (1) ◽  
pp. 83-90 ◽  
Author(s):  
Zhijian Li ◽  
Robert L. Jarret ◽  
Roy N. Pittman ◽  
Kerry B. Dunbar ◽  
James W. Demski
Keyword(s):  
Plant Regeneration ◽  
Culture Method ◽  
Nurse Culture ◽  
Arachis Paraguariensis

High frequency plant regeneration from rice protoplasts by novel nurse culture methods

1987 ◽  
Vol 206 (3) ◽  
pp. 408-413 ◽  
Author(s):  
Junko Kyozuka ◽  
Yasuyuki Hayashi ◽  
Ko Shimamoto
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Culture Methods ◽  
Nurse Culture

scholarly journals Explant-nurse Culture of Protoplasts and Plant Regeneration in the Radish.

1996 ◽  
Vol 13 (2) ◽  
pp. 127-132
Author(s):  
Kui Nan CAI ◽  
Toshinori ABE ◽  
Takeo SASAHARA
Keyword(s):  
Plant Regeneration ◽  
Nurse Culture

Application of nurse culture for plant regeneration from protoplasts of Lilium japonicum thunb

2006 ◽  
Vol 42 (3) ◽  
pp. 252-255 ◽  
Author(s):  
Fuminori Komai ◽  
Hitomi Morohashi ◽  
Mitsugu Horita
Keyword(s):  
Plant Regeneration ◽  
Nurse Culture

scholarly journals The effect of copper on plant regeneration in barley microspore culture

2017 ◽  
Vol 53 (No. 1) ◽  
pp. 17-22 ◽  
Author(s):  
K. Makowska ◽  
S. Oleszczuk ◽  
J. Zimny
Keyword(s):  
Plant Regeneration ◽  
Copper Sulphate ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Spring Barley ◽  
Culture Method ◽  
Induction Medium ◽  
Isolated Microspore Culture ◽  
Albino Plants ◽  
Effect Of Copper

Isolated microspore culture is an excellent system for the production of doubled haploids in many crops, including barley. In a more traditional barley anther culture method copper sulphate is known to enhance plant regeneration. Here we report that one hundred times higher concentration of copper sulphate in the isolated microspore culture of two spring barley genotypes compared to the standard content in the induction medium resulted in a 34% increase of total plant regeneration. Detailed analysis of plant regeneration showed that additional supplementation of copper sulphate increased not only the regeneration of green plants but also proportionately that of albino plants. Hence, the results from two studied genotypes do not support an assumption that the addition of copper reduces albinism in barley microspore culture.

Automated cell counting of astrocytes on patterned substrates containing aliphatic and charged properties

1995 ◽  
Vol 53 ◽  
pp. 974-975
Author(s):  
W. Shain ◽  
H. Ancin ◽  
H.C. Craighead ◽  
M. Isaacson ◽  
L. Kam ◽  
...  
Keyword(s):  
Cell Culture ◽  
Cell Attachment ◽  
Culture Method ◽  
Cell Counting ◽  
Data Sets ◽  
Nuclear Staining ◽  
Double Positive ◽  
A Cell ◽  
Wafer Test ◽  
Cell Densities

Neural protheses have potential to restore nervous system functions lost by trauma or disease. Nanofabrication extends this approach to implants for stimulating and recording from single or small groups of neurons in the spinal cord and brain; however, tissue compatibility is a major limitation to their practical application. We are using a cell culture method for quantitatively measuring cell attachment to surfaces designed for nanofabricated neural prostheses.Silicon wafer test surfaces composed of 50-μm bars separated by aliphatic regions were fabricated using methods similar to a procedure described by Kleinfeld et al. Test surfaces contained either a single or double positive charge/residue. Cyanine dyes (diIC18(3)) stained the background and cell membranes (Fig 1); however, identification of individual cells at higher densities was difficult (Fig 2). Nuclear staining with acriflavine allowed discrimination of individual cells and permitted automated counting of nuclei using 3-D data sets from the confocal microscope (Fig 3). For cell attachment assays, LRM5 5 astroglial cells and astrocytes in primary cell culture were plated at increasing cell densities on test substrates, incubated for 24 hr, fixed, stained, mounted on coverslips, and imaged with a 10x objective.

“Blastosphere”: A new culture method for human fetal hepatic progenitor cells

2001 ◽  
Vol 120 (5) ◽  
pp. A542-A543
Author(s):  
T HIROSE ◽  
K YASUCHIKA ◽  
T FUJIKAWA ◽  
H FUJII ◽  
S OE ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Culture Method ◽  
Hepatic Progenitor Cells

Plant regeneration from protoplasts of sugar beet (Beta vulgaris)

1995 ◽  
Vol 94 (2) ◽  
pp. 342-350 ◽  
Author(s):  
Steffen Lenzner ◽  
Kurt Zoglauer ◽  
Otto Schieder
Keyword(s):  
Plant Regeneration ◽  
Sugar Beet ◽  
Beta Vulgaris
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