High frequency plant regeneration from rice protoplasts by novel nurse culture methods

1987 ◽  
Vol 206 (3) ◽  
pp. 408-413 ◽  
Author(s):  
Junko Kyozuka ◽  
Yasuyuki Hayashi ◽  
Ko Shimamoto
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Culture Methods ◽  
Nurse Culture

High frequency plant regeneration from cotyledon and hypocotyl explants of ornamental kale

2009 ◽  
Vol 53 (4) ◽  
pp. 769-773 ◽  
Author(s):  
X. G. Dai ◽  
X. P. Shi ◽  
Y. M. Ye ◽  
Q. Fu ◽  
M. Z. Bao
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Hypocotyl Explants ◽  
Ornamental Kale

High-frequency plant regeneration from embryogenic cell suspension cultures of Gynura procumbens

2018 ◽  
Vol 13 (1) ◽  
pp. 27-33 ◽  
Author(s):  
Eun Yee Jie ◽  
Nornita Sham Atong ◽  
Woo Seok Ahn ◽  
Myung Suk Ahn ◽  
Byung Hwan Min ◽  
...  
Keyword(s):  
Plant Regeneration ◽  
Cell Suspension ◽  
High Frequency ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Embryogenic Cell Suspension ◽  
Embryogenic Cell

scholarly journals High Frequency Plant Regeneration of Musa paradisiaca cv. Karibale Monthan

2015 ◽  
Vol 3 (2) ◽  
pp. 202-209 ◽  
Author(s):  
R. Shashi Kumar ◽  
V. Krishna ◽  
. Venkatesh
Keyword(s):  
Plant Regeneration ◽  
High Frequency ◽  
Synergetic Effect ◽  
Multiple Shoot ◽  
Coconut Water ◽  
Morphological Variations ◽  
Musa Paradisiaca ◽  
Shoot Initiation

High frequency plant regeneration protocol has been standardized from banana cultivar Musa paradisiaca cv. Karibale Monthan, an endemic cultivar of Malnad region of Karnataka. The fruits are used as glomerular protective to solve kidney problems. To minimize the microbial contamination and to promote healthy growth, explants were treated with 70 % absolute alcohol for 6 min, 0.1 % Mercuric chloride for 10 min and 0.2 % for 10 min, 1 % Sodium hypochlorite for 15 min, 0.1 % Cefotaxime for 5 min and 0.05 % Gentamicin for 5 min. The high frequency shoot initiation (93.33 %) was recorded at 5 mg/l BAP. The synergetic effect of BAP (4 to 6 mg/l), TDZ (0.1 to 1.2 mg/l) and coconut water (0.1 to 0.9 ml/l) induced multiple shoot buds and it was optimized at the concentration of 5 mg/l BAP, 0.5 mg/l TDZ and 0.5 ml/l coconut water with 15.90 ± 1.66 frequency of shoots per propagule. Supplementation of 1.0 mg/l IBA induced 5.33 ± 1.21 numbers of roots with a mean root length of 7.50 ± 1.87 roots. The 99% of plantlets with distinct roots and shoots were successfully acclimatized in the green house and transferred to the field to evaluate the agro-morphological variations. The weight of the bunch (kg), number of hands in a bunch, number of fingers in a hand, length of the finger (cm), girth of the finger (cm) and girth of the pseudostem (cm) exhibited by in vitro plants were higher than the in vivo plants.Int J Appl Sci Biotechnol, Vol 3(2): 202-209 DOI: http://dx.doi.org/10.3126/ijasbt.v3i2.12536 

scholarly journals High frequency plant regeneration from embryogenic callus of a popular indica rice (Oryza sativa L.)

2009 ◽  
Vol 15 (4) ◽  
pp. 371-375 ◽  
Author(s):  
Alagarsamy Karthikeyan ◽  
Shunmugiah Thevar Karutha Pandian ◽  
Manikandan Ramesh
Keyword(s):  
Oryza Sativa ◽  
Plant Regeneration ◽  
High Frequency ◽  
Embryogenic Callus ◽  
Oryza Sativa L ◽  
Indica Rice
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