Modification of cell development in vitro: The effect of colchicine on anther and isolated microspore culture in Brassica napus

1995 ◽  
Vol 40 (3) ◽  
pp. 255-270 ◽  
Author(s):  
M. Zaki ◽  
H. Dickinson
Keyword(s):  
Brassica Napus ◽  
Microspore Culture ◽  
Cell Development ◽  
Isolated Microspore Culture ◽  
Development In Vitro

ICSBP/IRF-8 retrovirus transduction rescues dendritic cell development in vitro

Blood ◽  
2003 ◽  
Vol 101 (3) ◽  
pp. 961-969 ◽  
Author(s):  
Hideki Tsujimura ◽  
Tomohiko Tamura ◽  
Celine Gongora ◽  
Julio Aliberti ◽  
Caetano Reis e Sousa ◽  
...  
Keyword(s):  
Progenitor Cells ◽  
Consensus Sequence ◽  
Myeloid Cell ◽  
Cell Development ◽  
Interleukin 12 ◽  
Final Maturation ◽  
Development In Vitro ◽  
Partner Proteins

Abstract Dendritic cells (DCs) develop from bone marrow (BM) progenitor cells and mature in response to external signals to elicit functions important for innate and adaptive immunity. Interferon consensus sequence binding protein (ICSBP; also called interferon regulatory factor 8 [IRF-8]) is a hematopoietic cell–specific transcription factor expressed in BM progenitor cells that contributes to myeloid cell development. In light of our earlier observation that ICSBP−/− mice lack CD8α+DCs, we investigated the role of ICSBP in DC development in vitro in the presence of Flt3 ligand. Immature ICSBP−/− DCs developed from BM progenitor cells showed assorted defects, did not mature in response to activation signals, and failed to express CD8α and interleukin 12 (IL-12) p40, a feature consistent with ICSBP−/− DCs in vivo. We show that retroviral introduction of ICSBP restores the development of immature DCs that can fully mature on activation signals. All the defects seen with ICSBP−/− DCs were corrected after ICSBP transduction, including the expression of CD8α and IL-12 p40 as well as major histocompatability complex class II and other costimulatory molecules. ICSBP is known to regulate gene expression by interacting with partner proteins PU.1 and IRFs, thereby binding to target elements ISRE and EICE. Analysis of a series of ICSBP mutants showed that the intact DNA-binding activity as well as the ability to interact with partner proteins are required for the restoration of DC development/maturation, pointing to the transcriptional function of ICSBP as a basis of restoration. Taken together, this study identifies ICSBP as a factor critical for both early differentiation and final maturation of DCs.

IN VITRO ANTHER AND ISOLATED MICROSPORE CULTURE AS TOOLS IN SWEET AND SPICE PEPPER BREEDING

2009 ◽  
pp. 61-64 ◽  
Author(s):  
A. Gémes Juhász ◽  
Z. Kristóf ◽  
P. Vági ◽  
C. Lantos ◽  
J. Pauk
Keyword(s):  
Microspore Culture ◽  
Isolated Microspore Culture ◽  
Pepper Breeding

scholarly journals Modelling germ cell development in vitro

2008 ◽  
Vol 14 (9) ◽  
pp. 501-511 ◽  
Author(s):  
A. J. Childs ◽  
P. T.K. Saunders ◽  
R. A. Anderson
Keyword(s):  
Germ Cell ◽  
Cell Development ◽  
Germ Cell Development ◽  
Development In Vitro

scholarly journals Removal of myeloid cytokines from the cellular environment enhances T-cell development in vitro

2013 ◽  
Vol 25 (10) ◽  
pp. 589-599 ◽  
Author(s):  
Monique F. M. A. Smeets ◽  
Charley Mackenzie-Kludas ◽  
Mahmood Mohtashami ◽  
Hui-Hua Zhang ◽  
Juan Carlos Zúñiga-Pflücker ◽  
...  
Keyword(s):  
T Cell ◽  
T Cell Development ◽  
Cell Development ◽  
Cellular Environment ◽  
Development In Vitro

scholarly journals Androgenesis of Red Cabbage in Isolated Microspore Culture In Vitro

Plants ◽  
2021 ◽  
Vol 10 (9) ◽  
pp. 1950
Author(s):  
Anna Mineykina ◽  
Ludmila Bondareva ◽  
Alexey Soldatenko ◽  
Elena Domblides
Keyword(s):  
Culture Medium ◽  
Microspore Culture ◽  
Doubled Haploids ◽  
Antioxidant Properties ◽  
F1 Hybrids ◽  
Anthocyanin Content ◽  
Isolated Microspore Culture ◽  
Culture In Vitro ◽  
Red Cabbage

Red cabbage belongs to the economically important group of vegetable crops of the Brassicaceae family. A unique feature of this vegetable crop that distinguishes it from other members of the family is its unique biochemical composition characterized by high anthocyanin content, which gives it antioxidant properties. The production mainly uses F1 hybrids, which require constant parental lines, requiring 6–7 generations of inbreeding. Culture of isolated microspores in vitro is currently one of the promising methods for the accelerated production of pure lines with 100% homozygosity. The aim of this study is to investigate the factors and select optimal parameters for successful induction of red cabbage embryogenesis in isolated microspore culture in vitro and subsequent regeneration of DH plants. As a result of research, for the first time, it was possible to carry out the full cycle of obtaining DH plants of red cabbage from the induction of embryogenesis to their inclusion in the breeding process. The size of buds containing predominantly microspores at the late vacuolated stage and pollen at the early bi-cellular stage has to be selected individually for each genotype, because the embryoid yield will be determined by the interaction of these two factors. In the six samples studied, the maximum embryoid yield was obtained from buds 4.1–4.4 mm and 4.5–5.0 mm long, depending on the genotype. Cultivation of microspores was carried out on liquid NLN culture medium with 13% sucrose. The maximum number of embryoids (173.5 ± 7.5 pcs./Petri dish) was obtained on culture medium with pH 5.8 and heat shock at 32 °C for 48 h. Successful embryoid development and plant regeneration by direct germination from shoot apical meristem were achieved on MS culture medium with 2% sucrose and 0.7% agar, supplemented with 6-benzylaminopurine at a concentration of 1 mg/L. Analysis of the obtained regenerated plants, which successfully passed the stage of adaptation to ex vitro conditions by flow cytometry, showed that most of them were doubled haploids (up to 90.9%). A low number of seeds produced by self-fertilization in DH plants was observed.

scholarly journals Effects of Genotype and Culture Conditions on Microspore Embryogenesis and Plant Regeneration in Brassica Rapa ssp. Rapa L.

Plants ◽  
2020 ◽  
Vol 9 (2) ◽  
pp. 278 ◽  
Author(s):  
Daria Shumilina ◽  
Dmitry Kornyukhin ◽  
Elena Domblides ◽  
Alexey Soldatenko ◽  
Anna Artemyeva
Keyword(s):  
Microspore Culture ◽  
Doubled Haploids ◽  
Genetic Disorder ◽  
Cold Treatment ◽  
Microspore Embryogenesis ◽  
Culture Conditions ◽  
Secondary Embryogenesis ◽  
Isolated Microspore Culture ◽  
Pure Lines

Turnip is a biennial crop and, consequently, the creation of pure lines for breeding is a time-consuming process. The production of pure turnip lines using doubled haploids produced in isolated microspore culture has not been sufficiently developed. The aim of the present work was to determine some key factors inducing embryogenesis in the isolated microspore culture of turnip, as well as investigating the manners of embryo development. It was shown that the acidity of the medium is an important factor in embryo production; different optimal pH levels ranging from 6.2 to 6.6 corresponded to individual genotypes. Such factors as the cold treatment of buds and the addition of activated charcoal to the nutrient medium increased the responsiveness of all genotypes studied. The turnip variety ‘Ronde witte roodkop herfst’ demonstrated a genetic disorder in the development of microspores; namely, non-separation of some microspores from tetrads. In the in vitro culture, each of the daughter microspores developed on its own. This indicates the dependence of the possibility of embryogenesis in the turnip microspore culture on the genotype. Results suggest that the initiation of secondary embryogenesis in primary embryos leads to an increase in the proportion of doubled haploid plants.

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