Embryogenic protoplast cultures of orange jessamine (Murraya paniculata) and their regeneration into plants flowering in vitro

1995 ◽  
Vol 41 (3) ◽  
pp. 277-279 ◽  
Author(s):  
Hasan Basri Jumin ◽  
Nobumasa Nito
Planta ◽  
1967 ◽  
Vol 72 (4) ◽  
pp. 355-370 ◽  
Author(s):  
Colette Nitsch ◽  
J. P. Nitsch

HortScience ◽  
1990 ◽  
Vol 25 (4) ◽  
pp. 474-477 ◽  
Author(s):  
W. Msikita ◽  
R.M. Skirvin ◽  
J.A. Juvik ◽  
W.E. Splittstoesser ◽  
N. Ali

Seeds of `Burpless Hybrid' cucumber (Cucumis sativus L.) were separated from their seedcoats and excised into pieces comprising an embryonic axis and two cotyledons. The seed pieces were disinfested and explanted on modified Murashige and Skoog medium with 16 combinations of BAP (2.0, 3.0, 4.0, 5.0 mg·liter-1) and NAA (0.0,0.1,0.2,0.3 mg·liter-1). On all media, embryonic axes germinated and grew into whole plants within 4 weeks, but did not flower. Cotyledons developed adventitious shoots and male flowers on most media. A few female flowers developed. The best shoot regeneration and the most female flowers were observed on medium with 2.0 mg BAP and 0.3 mg NAA/liter. One of the female flowers was pollinated in vitro and it developed a small fruit with viable seed.


2011 ◽  
Vol 10 (10) ◽  
pp. 925-929 ◽  
Author(s):  
Mohana Sundaram ◽  
Sivakumar . ◽  
Karthikeyan . ◽  
Bhuvaneshwari . ◽  
Aishwarya . ◽  
...  

2015 ◽  
Author(s):  
Abhijit Nath ◽  
Mrinal Kanti Bhattacharjee

Murraya paniculata is a potent ethno medicinally important plant used both in traditional medicine as an analgesic and for wood and its activity against S. aureus established its traditional uses too. Selaginella is also used traditionally in wounds, postpartum, menstrual disease against microbes Lycopodium is used in Rheumatism and disease of lungs and kidneys and our study also revealed the scientific base behind its uses. The result of our experiments showed that in vitro antimicrobial screening of extracts and pure compounds of Murraya paniculata indicated positive activity against Staphylococcus aureus. They showed acetone extracts of the plants showed in inhibition zone of 13mm, methanol extract showed in inhibition zone of 9mm and ethanol extract showed in inhibition zone of 7mm but the distilled water extract showed no inhibition zone. We also performed work on selaginella and lycopodium but got no inhibition zone.


Planta ◽  
1967 ◽  
Vol 72 (4) ◽  
pp. 371-384 ◽  
Author(s):  
Colette Nitsch ◽  
J. P. Nitsch

2021 ◽  
Vol 6 (3) ◽  
pp. 19-35
Author(s):  
Victor Gabriel Almada-Ruiz ◽  
◽  
Gabriel Antonio Lugo-García ◽  
Laura Delia Ortega-Arenas ◽  
Edgar Eduardo Mendoza-García ◽  
...  

In vitro effect of ethanolic extracts of Azadirachta indica seeds, Melia azedarach and Swietenia humilis (Meliaceae) were evaluated on the mortality, repellency and oviposition of Diaphorina citri (Hemiptera: Liviidae). Toxicity was determined by the placement of adults and nymphs on orange leaf discs (Citrus sinensis cv. Valencia) previously sprayed or immersed in the treatments. The repellency was estimated through an exposition of adults to the treated leaf, in an experimental arena for 24 h. In order to inhibit oviposition, Murraya paniculata sprouts sprinkled with 2.5 mL of solution were used. The ethanolic extracts from the three meliaceus showed toxicity on nymphs and adults; however, adults evidenced the toxic effect as of 100.0 mg/mL, while nymphs did at 4.10 mg/mL. With a repellent effect, the 100 mg/mL solution stands out, with 82 and 78% for A. indica and S. humilis, respectively; effect associated to the concentration and the time. The oviposition was affected by ethanolic extracts of S. humilis and A. indica at 100.0 mg/mL. It is inferred that the ethanol extracts from S. humilis and A. indica present potential to be considered in the development of alternatives for the integrated management of D. citri.


Author(s):  
Avinash A. Adsul ◽  
Jaykumar J. Chavan ◽  
Nikhil B. Gaikwad ◽  
Rajaram V. Gurav ◽  
Ghansham B. Dixit ◽  
...  

Abstract The study aimed to develop an efficient, rapid, and large-scale in vitro regeneration system for propagation, conservation, and restoration of an endemic and critically endangered herb, Ceropegia mohanramii. The cultures were established using nodal explants on Murashige and Skoog’s (MS) medium supplemented with 6-benzylaminopurine (BAP: 1.0 mg/l). Nodal buds cultured on MS medium supplemented with BAP (2.0 mg/l) along with indole-3-butyric acid (IBA, 0.5 mg/l) resulted with production of maximum number of shoots (17.1 ± 1.2) in hundred percent of the cultures. MS medium supplemented with BAP (2.0 mg/l) along with diverse concentrations of indole-3acetic acid (IAA) promoted the in vitro flowering. In vitro regenerated shoots were transferred to one-half MS medium fortified with singular supplementation of auxins, where IBA (1.5 mg/l) served optimal for production of maximum number of roots (5.7 ± 0.6). In vitro derived plantlets were hardened under controlled conditions in a glasshouse and subsequently transferred to soil. Over 1200 saplings were transplanted to eight different localities of the Western Ghats where over 76% survival is recorded after 1 year of transplantation.


Sign in / Sign up

Export Citation Format

Share Document