Study of Antimicrobial Activity of Ethnomedically Important Plant Murraya paniculata

2015 ◽  
Author(s):  
Abhijit Nath ◽  
Mrinal Kanti Bhattacharjee
Keyword(s):  
Methanol Extract ◽  
Water Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
No Inhibition ◽  
Murraya Paniculata ◽  
Pure Compounds ◽  
Acetone Extracts ◽  
Scientific Base

Murraya paniculata is a potent ethno medicinally important plant used both in traditional medicine as an analgesic and for wood and its activity against S. aureus established its traditional uses too. Selaginella is also used traditionally in wounds, postpartum, menstrual disease against microbes Lycopodium is used in Rheumatism and disease of lungs and kidneys and our study also revealed the scientific base behind its uses. The result of our experiments showed that in vitro antimicrobial screening of extracts and pure compounds of Murraya paniculata indicated positive activity against Staphylococcus aureus. They showed acetone extracts of the plants showed in inhibition zone of 13mm, methanol extract showed in inhibition zone of 9mm and ethanol extract showed in inhibition zone of 7mm but the distilled water extract showed no inhibition zone. We also performed work on selaginella and lycopodium but got no inhibition zone.

Functional Effects of Pomegranate Peel Extracts on Milk: Antibacterial Measurements, Antioxidant Activities, and Photochemical Characterizations

2021 ◽  
Vol 15 (5) ◽  
pp. 571-579
Author(s):  
Rokayya Sami ◽  
Ebtihal Khojah ◽  
Mona Alharbi ◽  
Amina A. M. Al-Mushhin ◽  
Mohammed Saeed Alkaltham ◽  
...  
Keyword(s):  
Antioxidant Activities ◽  
Radical Scavenging ◽  
Water Extract ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Pomegranate Peel ◽  
Bacteria Growth ◽  
Wonderful Variety ◽  
Ic50 Values

Consumers are interested in antimicrobial by-products from natural sources. The present study aims to estimate the antibacterial effects of different concentrations and solvent extracts such as methanol, ethanol, and water extract of pomegranate peels with the milk quality application in-vitro. Listeria monocytogens, E. coli, Salmonella, and Staphylococcus aureus were used as bacteria growth indicators in two pomegranate varieties (i.e., Wonderful and Francis). Besides, evaluating somephysical characterization, antibacterial and antioxidant activities. Results showed that the highest inhibition zone of 18.88 mm was observed with 90% ethanol extract of Wonderful variety against Listeria monocytogens, at 0, 12.5, 25, and 50 mg/mL concentrations, while the lowest inhibition zone of 11.29 mm was observed with 90% methanol extract of Francis variety, at 12.5 mg/mL concentration. The milk treated with WPE of Wonderful variety maintained the bacterial growth from 1.19 ±0.29 x 109 to 1.43 x 1011 CFU/mL at 4 °C. Punicalagin was the most abundant polyphenolic compound (165.05–190.43 µg/mL) followed by gallic acid (153.08–177.65 µg/mL), and p-Hydroxybenzoic acid (91.29–92.5 mg/mL). Acetaldehyde (23.09–27.15 PPM), followed by acetoin (9.65–15.33 PPM) were the most predominant volatile compounds. The WPE treated milk maintained the sensory evaluations such as taste, color, texture, and overall acceptance longer time longer time (i.e., 144 h) than FPE treated milk (i.e., 168 h) at 4°C. DPPH and ABTS radical scavenging of capacity (IC50 values) of FPE treated milk were found to be lower than WPE treated milk (i.e., 159.65 µg/mL and 131.87 µg/mL), respectively. While, FPE treated milk reported a higher viscosity content than WPE treated milk (13.11 cP). In conclusion, pomegranate peel extracts especially Wonderful variety may be used to prolong the shelf-life of milk in dairy products manufacturers.

scholarly journals High-Throughput Identification of Organic Compounds from Polygoni Multiflori Radix Praeparata (Zhiheshouwu) by UHPLC-Q-Exactive Orbitrap-MS

Molecules ◽  
2021 ◽  
Vol 26 (13) ◽  
pp. 3977
Author(s):  
Shaoyun Wang ◽  
Xiaozhu Sun ◽  
Shuo An ◽  
Fang Sang ◽  
Yunli Zhao ◽  
...  
Keyword(s):  
High Performance ◽  
Chemical Constituents ◽  
Water Extract ◽  
Ethanol Extract ◽  
In Vivo Studies ◽  
Polygonum Multiflorum ◽  
Q Exactive ◽  
Orbitrap Ms

Polygoni Multiflori Radix Praeparata (PMRP), as the processed product of tuberous roots of Polygonum multiflorum Thunb., is one of the most famous traditional Chinese medicines, with a long history. However, in recent years, liver adverse reactions linked to PMRP have been frequently reported. Our work attempted to investigate the chemical constituents of PMRP for clinical research and safe medication. In this study, an effective and rapid method was established to separate and characterize the constituents in PMRP by combining ultra-high performance liquid chromatography with hybrid quadrupole-orbitrap mass spectrometry (UHPLC-Q-Exactive Orbitrap-MS). Based on the accurate mass measurements for molecular and characteristic fragment ions, a total of 103 compounds, including 24 anthraquinones, 21 stilbenes, 15 phenolic acids, 14 flavones, and 29 other compounds were identified or tentatively characterized. Forty-eight compounds were tentatively characterized from PMRP for the first time, and their fragmentation behaviors were summarized. There were 101 components in PMRP ethanol extract (PMRPE) and 91 components in PMRP water extract (PMRPW). Simultaneously, the peak areas of several potential xenobiotic components were compared in the detection, which showed that PMRPE has a higher content of anthraquinones and stilbenes. The obtained results can be used in pharmacological and toxicological research and provided useful information for further in vitro and in vivo studies.

Phytochemical Screening and In Vitro Antibacterial Activity of Green Tea (Camellia Sinensis L) Extract Against Staphylococcus Epidermidis

2020 ◽  
Vol 4 (8) ◽  
pp. 261-266
Author(s):  
Hardian Bimanto ◽  
Yuyun Dwi Wahyuni ◽  
Diah Titik Mutiarawati ◽  
Lully Hanni Endarini
Keyword(s):  
Green Tea ◽  
Staphylococcus Epidermidis ◽  
Ethanol Extract ◽  
Inhibition Zone ◽  
Dilution Method ◽  
Phytochemical Screening ◽  
Normality Test ◽  
In Vitro Antibacterial Activity ◽  
Green Tea Leaves

This study aimed to determine phytochemical screening and ethanol extract activity of the green leaves against Staphylococcus epidermidis. The microbial activity of the ethanol extract was carried out using the diffusion and dilution method with concentrations used of 1.5%, 2.5%, 3.5%, 4.5%, and 5.5% accompanied by three repetitions. The results showed that the ethanol extract of green tea leaves contained alkaloids, saponins, steroids or triterpenoids, flavonoids, polyphenols, and tannins and had the largest inhibition zone diameter of 30mm at a concentration of 4.5%. KHM (Minimum Inhibitory Content) at a level of 4.5% and KBM (Minimum Kill Rate) at a concentration of 5.5%. Analysis of the data with the normality test then proceed with the Kruskal Wallis test obtained a value of P = 0.008. With the value P

Antioxidant Activities In Vitro of Extracts from the Fermented Chinese Jujube

2012 ◽  
Vol 554-556 ◽  
pp. 1211-1215
Author(s):  
Yana Yuan ◽  
Ping Ping Zhang ◽  
Jin Fu Liu ◽  
Li Jin Zhang ◽  
Tong Hai Liu
Keyword(s):  
Antioxidant Capacity ◽  
Active Substance ◽  
Antioxidant Activities ◽  
Water Extract ◽  
Ethanol Extract ◽  
Dose Dependence ◽  
Human Consumption ◽  
Chinese Jujube ◽  
Lower Capacity

Chinese Jujube has long been used for human consumption and medicinal purposes in China. The objective of this study was to extract active substance from the powder of fermented Chinese Jujube and to detect their antioxidant capacities. Macroreticular resin was used to separate FJ-1 from 60% ethanol extract, and silica gel column was used for further separation of FJ-2. Precipitation of CPJ was conducted by addition of 95% ethanol to the water extract. The ability to eliminate DPPH•, O2• and •OH of this component was analyzed. Their activities show dose dependence character. FJ-1, FJ-2 show excellent antioxidant capacity on eliminating DPPH• and •OH, but CPJ shows lower capacity. The capacity on eliminating O2• of CPJ is higher than FJ-1, FJ-2 at the same concentration.

scholarly journals Antifungal ability and decay resistance of Fokienia hodginsii heartwood extract and its inhibitory effect on Gloeophyllum trabeum

BioResources ◽  
2020 ◽  
Vol 15 (2) ◽  
pp. 2784-2799
Author(s):  
Jian-Fei Wu ◽  
Xue-Feng Zhuang ◽  
Hong-mei Yuan ◽  
Quan Li ◽  
Wei Wang ◽  
...  
Keyword(s):  
Methanol Extract ◽  
Mass Loss Rate ◽  
Water Extract ◽  
Decay Resistance ◽  
Inhibitory Effect ◽  
Hot Water ◽  
Gloeophyllum Trabeum ◽  
Test Pieces ◽  
Hot Water Extract ◽  
Acetone Extracts

The inhibitory ability of Fokienia hodginsii heartwood (FHH) extracts on Trametes versicolor (TV) and Gloeophyllum trabeum (GT) as well as the toxic effect of its heartwood extracts on GT were studied. The growth inhibition ability of the samples was analyzed using the growth rate method. The results showed that in the experiment of inhibiting TV, extracts using hot water had little effect, acetone extracts had the best inhibiting effect, and the lowest value of acetone EC50 was 0.409 g/L. The parameter EC50 is the concentration of the corresponding agent that inhibits the growth of 50% fungi. In the antifungal experiment of GT, methanol extract had the best inhibition effect, and the lowest EC50 value was 0.283 g/L. The antifungal effect of five solvent extracts of FHH was good when the concentration was 10% (w/w), and at this time, the mass loss rate of the test pieces was below 11%, all of them were Class I, indicating a strong antifungal level. After observing the samples of GT with SEM, it was found that the structure of methanol extract treatment was more complete and the antiseptic effect was better than that of the hot water extract treatment.

scholarly journals Effect of Ethanol Extract from Clove Flower (Syzygium aromaticum) on the Growth of Trichophyton rubrum in vitro

Biomedika ◽  
2021 ◽  
Vol 14 (1) ◽  
pp. 47-54
Author(s):  
Khusnul Khusnul ◽  
Pepin Meilani Hildawati ◽  
Dewi Peti Virgianti
Keyword(s):  
High Temperature ◽  
Experimental Method ◽  
Trichophyton Rubrum ◽  
Fungal Growth ◽  
Ethanol Extract ◽  
Chemical Compounds ◽  
Inhibition Zone ◽  
Syzygium Aromaticum ◽  
Traditional Medicines

Indonesia is one of the countries with a tropical climate that has high temperature and humidity, a good atmosphere for fungal growth so that fungi can be found somewhere. Fungus Trichophyton rubrum is a fungal disease that attacks the nails, skin, hair. One of the preventions of this disease is by giving traditional medicines, namely clove flowers (Syzygium aromaticum) which contain chemical compounds saponins, tannins, flavonoids. Serves as an antioxidant that can prevent dermatosis. The purpose of this study was to determine the inhibition power of clove flowers (Syzygium aromaticum) on the growth of Trichophyton rubrum fungi. This research was carried out an experimental method with the Kirby Bauer method. Concentration dilution of clove ethanol extract (Syzygium aromaticum) from concentration 10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90%, 100%. The results of this study showed that the clove ethanol extract (Syzygium aromaticum) inhibit the growth of Trychophyton rubrum fungus from the concentration of 10% inhibition zone 14 mm, 20% inhibition zone 26 mm, 30% inhibition zone 36 mm, 40% inhibition zone 41 mm, 50% 45 mm inhibition zone, 60% 46 mm inhibition zone, 70% 48 mm inhibition zone, 80% 49 mm inhibition zone, 90% 51.0 mm inhibition zone, 100% inhibition zone of 56 mm.

scholarly journals Antibacterial Activity of Buah Merah (Pandanus conoideus Lam.) Against Bacterial Oral Pathogen of Streptococcus sanguinis ATCC10556, Streptococcus mutans ATCC 25175, and Enterococcus faecalis ATCC 29212: An in Vitro Study

2020 ◽  
Vol 14 (1) ◽  
pp. 113-119 ◽  
Author(s):  
Lisda Damayanti ◽  
Ida Ayu Evaangelina ◽  
Avi Laviana ◽  
Yetty Herdiyati ◽  
Dikdik Kurnia
Keyword(s):  
Antibacterial Activity ◽  
Ethyl Acetate ◽  
Methanol Extract ◽  
Synergistic Effects ◽  
In Vitro Study ◽  
Inhibition Zone ◽  
Oral Bacteria ◽  
Inhibition Zones

Background: Caries and periodontitis are dental diseases caused by bacteria of S. sanguinis, S. mutans, and E. faecalis with three main etiological factors of the host, substrate, and time. Objective: This study proposed to investigate the antibacterial effects of Buah Merah (Pandanus conoideus Lam.) against oral bacteria of E.faecalis, S. mutans, and S. sanguinis. Materials and Methods: The Buah Merah was extracted with different solvents to yield n-hexane, ethyl acetate, methanol, and H2O extracts. The concentrations of single and mixture extracts were adjusted for antibacterial assay against bacteria of E. faecalis, S. mutans, and S. sanguinis strains through agar well diffusion assay with chlorhexidine, fosfomycin, and quercetin used as positive controls. Results: The ethyl acetate extract showed highest antibacterial activity against three oral bacterial of E. faecalis, S. mutans, and S. sanguinis with inhibition zones values of 9.3, 12.3, and 17.9 mm at 40%, respectively, together with their MIC and MBC values of 1250 & 2500, 0.312 & 0.625, and 0.312 & 0.625 ppm, respectively. For the formulation of extracts, combinations samples test gave various effects to different bacteria, with the best activity showed by methanol-ethyl acetate (M-Ea) extracts against S. mutans with an inhibition zone of 16.25 mm at 40 ppm. The strong and synergistic effect of methanol extract against S. mutans was supported by inhibition zones of the formulation of methanol extract-fosfomycin which showed an inhibition zone of 25.9 mm at 10 ppm. Conclusion: The extracts of Buah Merah demonstrated antibacterial activity against oral bacteria of E. faecalis, S. mutans, and S. sanguinis and gave important information for further in vivo clinical studies to determine the exact dosages and its effectiveness in practical application. These results prove the antimicrobial effects of Buah Merah extracts as alternative natural drugs with synergistic effects of active constituents.

Antibacterial Activity of Dacryodes edulis Seed Extracts on Food-Borne Pathogens

1970 ◽  
Vol 4 (1) ◽  
pp. 17-21 ◽  
Author(s):  
BA Omogbai ◽  
TO Eneh
Keyword(s):  
Escherichia Coli ◽  
Antibacterial Activity ◽  
Pharmaceutical Preparation ◽  
Water Extract ◽  
Ethanol Extract ◽  
Antibacterial Activities ◽  
Food Borne ◽  
Dacryodes Edulis ◽  
Seed Extracts

The antibacterial effect of aqueous and ethanolic seed extracts of Dacryodes edulis was studied using agar diffusion technique. Extracts tested at a final concentration 2000;g/ml produced in vitro antibacterial activities in assays against food-borne bacterial isolates of Bacillus subtilis, Staphylococcus aureus, Escherichia coli, Klebsiella aerogenes, Pseudomonas aeruginosa, Salmonella typhi and Proteus mirabilis. The highest zone of inhibition was obtained from ethanol extract at 2000;g/ml against Escherichia coli with a diameter of 28mm.The minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) of the water extract ranged between 62.5-500;g/ml and 125-1000;g/ml respectively. The MIC and MBC of the ethanol extract ranged between 31.25-250;g/ml and 62.5-500;g/ml. The percentage inhibition of the isolates at 1000;g/ml was more in the ethanol extract (65.4-95.5%) compared to the water extract (30-2-60.2%). This study shows that the extracts of Dacryodes edulis have potential usage as a food preservative and pharmaceutical preparation. Keywords: Dacryodes edulis, Antibacterial activity, Aqueous, Ethanolic, Seed extract

STUDY OF IN VITRO ANTI-OXIDANT AND ANTI-DIABETIC ACTIVITY BY MUSSAENDA MACROPHYLLA ROOT EXTRACTS

2020 ◽  
pp. 70-74
Author(s):  
DEEPAK SHRESTHA ◽  
JITENDRA PANDEY ◽  
CHIRANJIBI GYAWALI ◽  
MAHESH LAMSAL ◽  
SUNITA SHARMA ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Methanol Extract ◽  
Radical Scavenging ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
Time Interval ◽  
Root Extract ◽  
Glucose Diffusion ◽  
Anti Oxidant

Objective: The systematic study of effective alternative anti-diabetic drugs has great importance to manage diabetes as well as other oxidative stress-related diseases. According to previous research, root and bark of Mussaenda macrophylla plant has anti-microbial, anti-coagulant, anti-inflammatory, and hepatoprotective activity. Ethnomedicinal data shows that Mussaenda macrophylla is used to treat diabetes as well as oxidative stress. The objective of this research is to investigate in vitro anti-diabetic and anti-oxidant activity of root extract of Mussaenda macrophylla. Methods: DPPH free radical scavenging assay was used to detect anti-oxidant potency of ethanol and methanol root extract of the plant and expressed as % of radicle inhibition. Anti-diabetic activity was determined by the glucose diffusion method using a glucose oxidase kit and results were expressed as mean±SD. Results: The ethanol root extract at the concentration of 50 mg/ml and 100 mg/ml showed better glucose diffusion inhibition than that of methanol extract at the same concentration on increasing time interval. Ethanol extract at the concentration 100 µg/ml displayed better DPPH scavenging activity (89.83±0.19 %) than that of methanol extract (86.61±0.75%). Conclusion: This study concluded that ethanol and methanol root extract of Mussenda macrophylla have potent anti-diabetic as well as anti-oxidant activity but further advance research is necessary in the animal model.

Antibacterial Activity of Nanoparticles Produced by Ethanol Extract of Boesenbergia rotunda Rhizome Loaded with Chitosan and Alginic Acid Against Streptococcus mutans by In Vitro

2017 ◽  
Vol 7 (03) ◽  
Author(s):  
Sri Atun ◽  
Sri Handayani ◽  
Melia Aliffiana ◽  
Hajar Nur Afifah ◽  
Anna Rakhmawati
Keyword(s):  
Staphylococcus Aureus ◽  
Antibacterial Activity ◽  
Antibacterial Agent ◽  
Methanol Extract ◽  
Alginic Acid ◽  
Ethanol Extract ◽  
Diffusion Method ◽  
E Coli ◽  
Crude Methanol Extract

The antibacterial activity of three Cupressaceae plants (Thujaoccidentalis,ThujaorientalisandChamaecyparisobtusa) was tested against three bacteria using the agar diffusion method. The ether and ethylacetate fraction of crude methanol extract from the three plants showed potent antibacterial activity against the tested microorganisms. The result showed that Staphylococcus aureus revealed the most sensitivity among the tested bacteria. Thujaoccidentalisether fraction and Thujaorientalis hexane fraction exhibited the highest antibacterial activity against Staphylococcus aureus. E. coli was shown the highest MIC values compared to the other two tested bacteria, which indicates the lowest antibacterial activity against the bacterium. This study promises an interesting future for designing a potentially active antibacterial agent from the three Cupressaceae plants.

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