Evaluation of cassava (Manihot esculenta Crantz) germplasm collections using RAPD markers

Euphytica ◽  
1994 ◽  
Vol 74-74 (3) ◽  
pp. 203-209 ◽  
Author(s):  
Philippe Marmey ◽  
John R. Beeching ◽  
Serge Hamon ◽  
Andr� Charrier
Keyword(s):  
Manihot Esculenta ◽  
Rapd Markers ◽  
Manihot Esculenta Crantz ◽  
Germplasm Collections

ANALYSIS OF GENETIC DIVERSITY OF 26 CASSAVA VARIETIES (Manihot esculenta CRANTZ) WITH DIFFERENT RESPONSES TO WATERLOGGING CONDITIONS BY USING RAPD MARKERS

2021 ◽  
Vol 66 (3) ◽  
pp. 170-179
Author(s):  
Sengsoulichan Dethvongsa ◽  
Vu Nguyen Anh ◽  
Van Tran Khanh
Keyword(s):  
Genetic Diversity ◽  
Phylogenetic Tree ◽  
Manihot Esculenta ◽  
Rapd Markers ◽  
Flood Tolerance ◽  
Randomly Amplified Polymorphic Dna ◽  
Manihot Esculenta Crantz ◽  
Rapd Pcr ◽  
Cassava Production ◽  
Cassava Varieties

RAPD (Randomly Amplified Polymorphic DNA) is an indicator for high and stable polymorphism, widely used in the study of the diversity of cassava. In this paper, the results of using 20 polymorphic primers OPK combined with the establishment of the phylogenetic tree to analyze the genetic diversity of 26 cassava varieties with different responses to waterlogging conditions by using the RAPD-PCR technique were presented. The purpose of this experiment was to show the genetic relevance of the studied cassava varieties. The results showed that the flood tolerance of cassava was not related to the polymorphism and branching characteristics of the stem. This information may be use as a basis for selecting flood-tolerant cassava varieties for cassava production, as well as the basis for selecting genetically different parents for breeding.

scholarly journals Genetic diversity characterization of cassava cultivars (Manihot esculenta Crantz).: I) RAPD markers

1998 ◽  
Vol 21 (1) ◽  
pp. 105-113 ◽  
Author(s):  
Carlos Colombo ◽  
Gérard Second ◽  
Tereza Losada Valle ◽  
André Charrier
Keyword(s):  
Genetic Diversity ◽  
Multivariate Analysis ◽  
Genetic Variability ◽  
Manihot Esculenta ◽  
Core Collection ◽  
Rapd Markers ◽  
Manihot Esculenta Crantz ◽  
Important Resource ◽  
Flour Production

RAPD markers were used to investigate the genetic diversity of 31 Brazilian cassava clones. The results were compared with the genetic diversity revealed by botanical descriptors. Both sets of variates revealed identical relationships among the cultivars. Multivariate analysis of genetic similarities placed genotypes destinated for consumption "in nature" in one group, and cultivars useful for flour production in another. Brazil’s abundance of landraces presents a broad dispersion and is consequently an important resource of genetic variability. The botanical descriptors were not able to differentiate thirteen pairs of cultivars compared two-by-two, while only one was not differentiated by RAPD markers. These results showed the power of RAPD markers over botanical descriptors in studying genetic diversity, identifying duplicates, as well as validating, or improving a core collection. The latter is particularly important in this vegetatively propagated crop.

Strict maternal inheritance of RAPD fingerprints confirms apomixis in cassava (Manihot esculenta Crantz)

1996 ◽  
Vol 76 (2) ◽  
pp. 379-382 ◽  
Author(s):  
Dario Grattapaglia ◽  
Cynthia Costa e Silva ◽  
Nagib M. A. Nassar
Keyword(s):  
Key Words ◽  
Interspecific Hybrid ◽  
Manihot Esculenta ◽  
Rapd Markers ◽  
Maternal Inheritance ◽  
Random Amplified Polymorphic Dna ◽  
Molecular Evidence ◽  
Manihot Esculenta Crantz ◽  
Rapd Fingerprints

Undisputed molecular evidence for apomixis in cassava is presented. Based on genome sampling with 130 RAPD markers we confirmed apomixis for one offspring of a cassava clone derived from an interspecific hybrid. The probability that complete uniformity in RAPD fingerprints between mother and offspring occurred by chance was < 10−5. Key words: Apomixis, cassava, Manihot esculenta, random amplified polymorphic DNA

Identification of RAPD markers linked to pubescent trait in cassava (Manihot esculenta CRANTZ)

2009 ◽  
Vol 11 (1) ◽  
Author(s):  
V Oduro ◽  
O Safo-Kantanka ◽  
KE Danso ◽  
HM Amoatey ◽  
A Amiteye
Keyword(s):  
Manihot Esculenta ◽  
Rapd Markers ◽  
Manihot Esculenta Crantz

CONCENTRADO PROTEICO OBTIDO DAS FOLHAS DE MANDIOCA (Manihot esculenta Crantz) DE TRÊS VARIEDADES COMERCIAIS.

2017 ◽  
Vol 13 (1) ◽  
Author(s):  
Izabel Aparecida Soares ◽  
Mauro Sérgio Téo ◽  
Carlise DEBASTIANI ◽  
Suzymeire BARONI ◽  
Vanessa Silva RETUCI
Keyword(s):  
Manihot Esculenta ◽  
Crude Protein ◽  
Hydrocyanic Acid ◽  
Protein Concentrate ◽  
Manihot Esculenta Crantz ◽  
White Variety ◽  
Mean Values ◽  
Randomized Design ◽  
Significant Difference ◽  
The Mean

O trabalho teve por objetivo verificar diferenças entre rendimento do concentrado proteico e proteína bruta da folha da mandioca (Manihot esculenta Crantz), obtidos a partir de três variedades comerciais: branca, cascuda e vermelha. As manivas foram plantadas seguindo o delineamento experimental inteiramente casualizado com três repetições. Nas comparações entre as variedades, considerou coletas escalonadas pós-plantio, realizadas aos 12, 14 e 16 meses. O concentrado proteico foi obtido a partir da farinha das folhas inteiras e submetido ao método de termo - coagulação ácido e a proteína bruta pelo método padrão AOAC. Os dados foram submetidos a análise de variância e comparados pelo teste de Tukey - 5% de probabilidade. Os resultados não indicaram diferença significativa entre as médias obtidas para rendimento de concentrado proteico. Para a variável porcentagem de proteína bruta a variedade Branca foi a que apresentou maior valor, com média de 46,25%, seguida pela Cascuda e Vermelha, 44,52% e 37,30%, sucessivamente. Conclui-se que outros estudos devem avaliar condições que possam influenciar no teor de proteína foliar, como clima e solo, e, avaliar os níveis de ácido cianídrico de cada variedade, indicando qual das três é a melhor para a extração do concentrado proteico das folhas. Palavras chaves: Manihot esculenta Crantz, variedades comerciais, concentrado proteico, proteína bruta. ABSTRACT: The study aimed to assess the differences between income protein concentrate, crude protein of cassava leaf (Manihot esculenta Crantz), obtained from three commercial varieties: white, red and cascuda. The cuttings were planted following the completely randomized design with three replications. Comparisons between the varieties considered after planting staggered collections, held on 12, 14 and 16 months. The protein concentrate was obtained from flour of whole sheets and subjected to the term method - acid coagulation and crude protein by AOAC standard method. The data were submitted to ANOVA and Tukey test - 5% probability. The results indicated no significant difference between the mean values obtained for protein concentrate income. For the variable percentage of crude protein White variety showed the highest, with an average of 46.25%, followed by cascuda and Red, 44.52% and 37.30%, successively. We conclude that further studies should evaluate conditions that may affect the leaf protein content, such as climate and soil, and evaluate the hydrocyanic acid levels of each variety, indicating which of the three is the best for the extraction of protein concentrate from leaves Key words: Manihot esculenta Crantz, commercial varieties, protein concentrate, crude protein.

MODELAGEM DA CINÉTICA DE SECAGEM EM FÉCULAS DE MANDIOCA (MANIHOT ESCULENTA CRANTZ)

2018 ◽  
Author(s):  
Barros Sâmela Leal ◽  
Santos Newton Carlos ◽  
Araújo Thaís Jaciane ◽  
Melo Mylena Olga Pessoa ◽  
Nascimento Amanda Priscila Silva
Keyword(s):  
Manihot Esculenta ◽  
Manihot Esculenta Crantz

CONJUNTURA DO MERCADO DA MANDIOCA (Manihot esculenta Crantz) NO BRASIL E NO ESTADO DO PARÁ

2018 ◽  
Author(s):  
Brochado Maura Gabriela da Silva ◽  
Botelho Matheus Gabriel Lopes ◽  
Souza Camila Eduarda Sousa de ◽  
Nunes Mariana Lameira ◽  
Ferreira Leonardo Elias
Keyword(s):  
Manihot Esculenta ◽  
Manihot Esculenta Crantz

Seleksi Ubi Kayu (Manihot esculenta Crantz.) Lokal Berdaya Hasil Tinggi Asal Indonesia berdasarkan Karakter Umbi

2018 ◽  
Vol 1 (1) ◽  
pp. 10-16
Author(s):  
Fadhillah Laila ◽  
Budi Waluyo ◽  
Agung Kurniawan
Keyword(s):  
Manihot Esculenta ◽  
Manihot Esculenta Crantz

Indonesia memiliki kekayaan plasma nutfah ubi kayu yang tersebar di seluruh wilayah Indonesia. Ubi kayu mengandung karbohidrat tinggi dan berperan dalam diversifikasi pangan. Tujuan dari penelitian ini adalah menyeleksi 80 varietas ubi kayu budidaya lokal asal berbagai wilayah di Indonesia yang berpotensi hasil tinggi. Penelitian ini menggunakan 80 aksesi ubi kayu dari seluruh pulau-pulau besar di Indonesia yang disusun dalam rancangan Augmented dengan 3 tanaman kontrol per baris. Penelitian dilakukan dari Juli 2013-November 2014. Hasil penelitian menunjukkan bahwa keragaman genetik dan fenotipik yang luas pada ubi kayu asal Indonesia berdasarkan karakter umbi. Potensi genetik berdasarkan karakter hasil dan komponen hasil terkategorikan tinggi dengan nilai heritabilitas 0,59-0,75. Uji lanjut LSI (Least Significant Increase) menyeleksi aksesi yang memiliki daya hasil tinggi pada karakter umbi diantaranya karakter jumlah ubi/tanaman pada aksesi 563, 570, dan 599. Karakter bobot ubi/tanaman pada aksesi 507,563, 598, dan 541. Karakter bobot/ubi pada aksesi aksesi 534,528 dan 541. Karakter bobot ubi/plot pada aksesi 629. Karakter potensi hasil pada aksesi 629. Hal ini dapat memberikan informasi mengenai diversitas genetik aksesi-aksesi ubi kayu lokal Indonesia sehingga dapat dievaluasi untuk didapatkan hasil tinggi ubi kayu untuk diversifikasi pangan.

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