Identification of somatic hybrids of dihaploid Solanum tuberosum lines and S. brevidens by species specific RAPD patterns and assessment of disease resistance of the hybrids

Euphytica ◽  
1994 ◽  
Vol 80 (3) ◽  
pp. 207-217 ◽  
Author(s):  
Veli-Matti Rokka ◽  
Yong-Sheng Xu ◽  
Jyri Kankila ◽  
Anja Kuusela ◽  
Seppo Pulli ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Disease Resistance ◽  
Somatic Hybrids ◽  
Species Specific

Somatic hybridization between Solanum tuberosum and S. cardiophyllum

2006 ◽  
Vol 86 (2) ◽  
pp. 539-545 ◽  
Author(s):  
Y. Z. Shi ◽  
Q. Chen ◽  
H. Y. Li ◽  
D. Beasley ◽  
D. R. Lynch
Keyword(s):  
Solanum Tuberosum ◽  
Somatic Hybrids ◽  
Rapd Marker ◽  
Endosperm Balance Number ◽  
Parental Line ◽  
Cultivated Potato ◽  
Hybrid Plants ◽  
Young Leaves ◽  
The Difference ◽  
Species Specific

The wild diploid Mexican species, Solanum cardiophyllum Lindl. (2n = 2x = 24), is resistant to important potato diseases. However, introgression of resistance to the tetraploid cultivated potato (S. tuberosum L.) (2n = 4x = 48) by conventional crossing is not feasible due to the difference in their endosperm balance number between these species. Somatic hybrids between S. cardiophyllum and S. tuberosum were produced for the first time by electrofusion of protoplasts isolated from young leaves of each parental line. The hybrid nature of the regenerated plants was confirmed based on morphology, chromosome number and DNA species-specific RAPD markers. All the somatic hybrids produced a violet pigmentation on their stems and petioles, which resembled the wild partner. Most of the hybrid plants had 2n = 72 chromosomes and exhibited a morphology intermediate between the two fusion parents, but with a tendency towards cultivated potato. These plants flowered and set fruit when backcrossed with their S. tuberosum fusion parent. Key words: Potato (Solanum tuberosum), S. cardiophyllum, protoplast fusion, RAPD marker

A specific oligonucleotide of the 5S rDNA spacer and species-specific elements identify symmetric somatic hybrids between Solanum tuberosum and S. pinnatisectum

1995 ◽  
Vol 90 (5) ◽  
pp. 720-726 ◽  
Author(s):  
C. Zanke ◽  
N. Borisjuk ◽  
B. Ruoss ◽  
L. Schilde-Rentschler ◽  
H. Ninnemann ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Somatic Hybrids ◽  
5S Rdna ◽  
Species Specific

Somatic hybrids between potato Solanum tuberosum and wild species Solanum verneï exhibit a recombination in the plastome

2005 ◽  
Vol 83 (1) ◽  
pp. 1-11 ◽  
Author(s):  
S. Trabelsi ◽  
R. Gargouri-Bouzid ◽  
F. Vedel ◽  
A. Nato ◽  
L. Lakhoua ◽  
...  
Keyword(s):  
Solanum Tuberosum ◽  
Wild Species ◽  
Somatic Hybrids ◽  
Solanum Vernei

Chromosome pairing and potential for intergeneric recombination in some hypotetraploid somatic hybrids of Lycopersicon esculentum (+) Solanum tuberosum

Genome ◽  
1993 ◽  
Vol 36 (6) ◽  
pp. 1032-1041 ◽  
Author(s):  
J. H. de Jong ◽  
A. M. A. Wolters ◽  
J. M. Kok ◽  
H. Verhaar ◽  
J. van Eden
Keyword(s):  
Solanum Tuberosum ◽  
Lycopersicon Esculentum ◽  
Synaptonemal Complex ◽  
Chromosome Pairing ◽  
Somatic Hybrids ◽  
Cytogenetic Study ◽  
Unreduced Gametes ◽  
Root Tip ◽  
Prophase I ◽  
Metaphase I

Three somatic hybrids resulting from protoplast fusions of a diploid kanamycin-resistant line of tomato (Lycopersicon esculentum) and a dihaploid hygromycin-resistant transformant of a monohaploid potato (Solanum tuberosum) line were used for a cytogenetic study on chromosome pairing and meiotic recombination. Chromosome counts in root-tip meristem cells revealed two hypotetraploids with chromosome complements of 2n = 46 and one with 2n = 47. Electron microscope analyses of synaptonemal complex spreads of hypotonically burst protoplasts at mid prophase I showed abundant exchanges of pairing partners in multivalents involving as many as eight chromosomes. In the cells at late pachytene recombination nodules were found in multivalents on both sides of pairing partner exchanges, indicating recombination at both homologous and homoeologous sites. Light microscope observations of pollen mother cells at late diakinesis and metaphase I also revealed multivalents, though their occurrence in low frequencies betrays the reduction of multivalent number and complexity. Precocious separation of half bivalents at metaphase I and lagging of univalents at anaphase I were observed frequently. Bridges, which may result from an apparent inversion loop found in the synaptonemal complexes of a mid prophase I nucleus, were also quite common at anaphase I, though the expected accompanying fragments could be detected in only a few cells. Most striking were the high frequencies of first division restitution in preparations at metaphase II/anaphase II, giving rise to unreduced gametes. In spite of the expected high numbers of balanced haploid and diploid gametes, male fertility, as revealed by pollen staining, was found to be negligible.Key words: synaptonemal complex, recombination, chromosome pairing, somatic hybrid, Lycopersicon esculentum (+) Solanum tuberosum.

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