DNA fingerprinting in soybean (Glycine max (L.) Merrill) with oligonucleotide probes for simple repetitive sequences

Euphytica ◽  
1994 ◽  
Vol 80 (1-2) ◽  
pp. 129-136 ◽  
Author(s):  
T. Yanagisawa ◽  
M. Hayashi ◽  
A. Hirai ◽  
K. Harada
Keyword(s):  
Glycine Max ◽  
Dna Fingerprinting ◽  
Repetitive Sequences ◽  
Oligonucleotide Probes ◽  
Simple Repetitive Sequences ◽  
Glycine Max L

Microsatellite fingerprinting in the genus Phaseolus

Genome ◽  
1995 ◽  
Vol 38 (3) ◽  
pp. 507-515 ◽  
Author(s):  
Andrea Hamann ◽  
Dorothea Zink ◽  
Walter Nagl
Keyword(s):  
Genetic Variability ◽  
Dna Fingerprinting ◽  
Intraspecific Variation ◽  
Repetitive Sequences ◽  
The Other ◽  
Gene Pools ◽  
Oligonucleotide Probes ◽  
Banding Patterns ◽  
Simple Repetitive Sequences ◽  
Almost All

The genetic variability of the genus Phaseolus was investigated by nonradioactive DNA fingerprinting. The simple repetitive sequences (GATA)4, (GACA)4, (CAC)5, and (CA)8 were used as probes to differentiate 18 species comprised of 90 genotypes. (GATA)4, (CAC)5, and (CA)8 could be detected in the genome of nearly all species, while the (GACA)4 motif occurred only in 13 species. Almost all fragments that hybridized with (GACA)4 also hybridized with (GATA)4. All but two cultivars of Phaseolus vulgaris, P. lunatus, P. acutifolius, and P. polyanthus showed specific banding patterns with (GATA)4. The other repetitive motifs revealed only limited or no intraspecific variation. In P. vulgaris, two group-specific patterns were found with (GATA)4, giving further evidence for a Middle American and an Andean origin of the P. vulgaris genotypes. The high intraspecific pattern variation that was revealed with (GATA)4 in the predominantly self-pollinating species P. vulgaris and P. lunatus can probably be explained by there being at least two primary centres of domestication and, hence, genetic diversification. In cross-pollinating species (e.g., P. coccineus), the observed intraspecific variation was, surprisingly, rather low. The present study shows that DNA fingerprinting with microsatellites successfully distinguishes among gene pools, cultivars, and, in some cases, among individuals.Key words: Leguminosae, plants, nonradioactive, simple sequences, digoxigenated oligonucleotide probes.

Prevalence of the pit and antipit in the genus Glycine

1987 ◽  
Vol 45 ◽  
pp. 986-987
Author(s):  
R. W. Yaklich ◽  
E. L. Vigil ◽  
W. P. Wergin
Keyword(s):  
Amino Acids ◽  
Endoplasmic Reticulum ◽  
Glycine Max ◽  
Seed Coat ◽  
Cell Types ◽  
Abaxial Surface ◽  
Legume Seed ◽  
Initial Report ◽  
Cone Cells ◽  
Glycine Max L

The legume seed coat is the site of sucrose unloading and the metabolism of imported ureides and synthesis of amino acids for the developing embryo. The cell types directly responsible for these functions in the seed coat are not known. We recently described a convex layer of tissue on the inside surface of the soybean (Glycine max L. Merr.) seed coat that was termed “antipit” because it was in direct opposition to the concave pit on the abaxial surface of the cotyledon. Cone cells of the antipit contained numerous hypertrophied Golgi apparatus and laminated rough endoplasmic reticulum common to actively secreting cells. The initial report by Dzikowski (1936) described the morphology of the pit and antipit in G. max and found these structures in only 68 of the 169 seed accessions examined.

PENGARUH PEMBERIAN PUPUK ORGANIK CAIR ASAL LIMBAH TERHADAP SERAPAN HARA SERTA PRODUKSI TANAMAN KEDELAI DI LAHAN GAMBUT

2017 ◽  
Vol 2 (02) ◽  
pp. 204-218
Author(s):  
Hendra Saputra ◽  
Intan Sari ◽  
Muhammad Arfah
Keyword(s):  
Glycine Max ◽  
Glycine Max L

Penelitian tentang pengaruh pemberian Pupuk organik cair (POC) asal limbah tumbuhan terhadap serapan hara N dan P serta produksi tanaman kedelai (Glycine max (L) Merrill) di lahan gambut telah dilaksanakan di kampus II Unisi Fakultas Pertanian Jl. Lintas Propinsi Parit 01, Desa Pulau Palas, Kecamatan Tembilahan Hulu, Kabupaten Indragiri Hilir Propinsi Riau. Dimulai dari bulan Agustus sampai bulan Oktober 2013. Tujuan dari penelitian ini untuk mendapatkan POC asal limbah tumbuhan yang terbaik untuk serapan hara N dan P serta produksi tanaman kedelai di lahan gambut. Penelitian ini menggunakan rancangan acak kelompok (RAK) faktor tunggal dengan 7 perlakuan dan 4 ulangan, 2 tanaman dijadikan sampel. Perlakuan dosis POC limbah tanaman pisang dan POC limbah sayur kol yang diberikan yaitu 0 L/Ha, 200 L/Ha, 400 L/Ha dan 600 L/Ha. Parameter pengamatan yaitu : serapan hara N dan P pada fase awal generatif, tinggi tanaman, jumlah bintil akar, polong hampa, produksi perplot, berat 100 biji dan brangkasan kering. Data pengamatan dianalisis dengan sidik ragam (ANOVA) dan dilanjutkan dengan Uji Lanjut Tukey HSD pada taraf 5%. Berdasarkan penelitian yang telah dilaksanakan dapat disimpulkan bahwa pemberian POC asal limbah tumbuhan tidak berpengaruh nyata terhadap serapan hara N dan P, tinggi tanaman, jumlah bintil akar, polong hampa, brangkasan kering tetapi berpengaruh nyata terhadap produksi perplot dan berat 100 biji.

PERTUMBUHAN DAN HASIL SEMBILAN KULTIVAR KEDELAI (Glycine max (L.) Merrrill) DI LAHAN SAWAH

2018 ◽  
Vol 1 (2) ◽  
pp. 96
Author(s):  
Siti Wahyuni ◽  
Umi Trisnaningsih ◽  
Meilina Prasetyo
Keyword(s):  
Glycine Max ◽  
Glycine Max L

COPPER AND ZINC INDUCED CHANGES IN SOYBEAN (Glycine max (L.) Merr.)

1970 ◽  
pp. 09
Author(s):  
K. SANKAR GANESH ◽  
P. SUNDARAMOORTHY
Keyword(s):  
Heavy Metals ◽  
Glycine Max ◽  
Aquatic Environment ◽  
Morphological Traits ◽  
Industrial Activities ◽  
Copper And Zinc ◽  
Glycine Max L ◽  
Zinc Concentrations ◽  
Induced Changes

Heavy metals are one of the most important pollutants released to the aquatic environment by the various industrial activities. The use of these wastewater for irrigation results accumulation of heavy metals in soil and plants. So, the present investigation deals with the various concentrations (0, 5, 10, 25, 50, 100, 200 and 300 mg/l) of copper and zinc on germination studies of soybean. The different concentrations of copper and zinc were used for germination studies. The seedlings were allowed to grow upto seven days. The studied morphological traits increased at 5 mg/l concentration and these parameters are gradually decreased with the increase of copper and zinc concentrations.

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