Induction of an alloplasmic male-sterile Brassica oleracea by substituting cytoplasm from ?Early Scarlet Globe? radish (Raphanus sativus)

Euphytica ◽  
1981 ◽  
Vol 30 (3) ◽  
pp. 855-859 ◽  
Author(s):  
Gilbert D. McCollum
Keyword(s):  
Brassica Oleracea ◽  
Raphanus Sativus ◽  
Male Sterile

Development of male sterile Eruca sativa carrying a Raphanus sativus/Brassica oleracea cybrid cytoplasm

2015 ◽  
Vol 129 (2) ◽  
pp. 331-344 ◽  
Author(s):  
Thomas Nothnagel ◽  
Evelyn Klocke ◽  
Otto Schrader ◽  
Bettina Linke ◽  
Holger Budahn
Keyword(s):  
Brassica Oleracea ◽  
Raphanus Sativus ◽  
Eruca Sativa ◽  
Male Sterile

scholarly journals Sound waves affect the total flavonoid contents in Medicago sativa , Brassica oleracea and Raphanus sativus sprouts

2019 ◽  
Vol 100 (1) ◽  
pp. 431-440 ◽  
Author(s):  
Joo Yeol Kim ◽  
Ye Eun Kang ◽  
Soo In Lee ◽  
Jin A Kim ◽  
Muthusamy Muthusamy ◽  
...  
Keyword(s):  
Medicago Sativa ◽  
Brassica Oleracea ◽  
Raphanus Sativus ◽  
Total Flavonoid ◽  
Sound Waves

scholarly journals Development of male sterile broccoli lines with raphanus sativus cytoplasm and assessment of their value for breeding purposes

2013 ◽  
Vol 21 (2) ◽  
pp. 101-107 ◽  
Author(s):  
Piotr Kamiński
Keyword(s):  
Cytoplasmic Male Sterility ◽  
Male Sterility ◽  
Raphanus Sativus ◽  
Morphological Characters ◽  
F1 Hybrids ◽  
Vegetable Crops ◽  
Male Sterile ◽  
Self Compatibility ◽  
High Level ◽  
Research Institute

ABSTRACT The aim of this study was to obtain new broccoli lines with cytoplasmic male sterility trait for the development of the modern F1 hybrids. CDT70 cauliflower line obtained in the Research Institute of Horticulture, formerly Research Institute of Vegetable Crops, Skierniewice, Poland, with the reliable cytoplasmic male sterility from Raphanus sativus (Ogu-INRA) was selected as a source of this trait. Three broccoli lines: BMi, BCr1 and BCr2 were used as donors of commercial characters in all cross combinations with sterile components. Selected fertile broccoli genotypes were characterized by good quality, uniformity and high level of self-compatibility. The breeding procedure included three consecutive back crosses of male sterile genotypes with fertile broccoli lines that lasted from 2008 to 2012. In each generation, self-compatibility level, the stability of the male sterility trait and ability for the generative propagation of back-crossed genotypes were tested in comparison with donor broccoli lines in the greenhouse. The agronomical and morphological characters of the back-crossed progeny were also evaluated in the field. As a result, three CMS broccoli lines of Bc3 generation with good quality and high seeding index, suitable for the breeding purposes, were obtained.

In vitro ovule culture of intergeneric hybrids between Brassica oleracea and Raphanus sativus

1990 ◽  
Vol 41 (3) ◽  
pp. 181-188 ◽  
Author(s):  
Mohammad Mofazzal Hossain ◽  
Haruhisa Inden ◽  
Tadashi Asahira
Keyword(s):  
Brassica Oleracea ◽  
Raphanus Sativus ◽  
Intergeneric Hybrids ◽  
Ovule Culture

scholarly journals Genome-Wide Identification of GH3 Genes in Brassica Oleracea and Identification of a Promoter Region for Anther-Specific Expression of a GH3 Gene

2020 ◽  
Author(s):  
Hankuil Yi ◽  
Jiseong Jeong ◽  
Sunhee Park ◽  
Jeong Hui Im
Keyword(s):  
Brassica Oleracea ◽  
Promoter Region ◽  
Expression Patterns ◽  
Leaf Primordia ◽  
Start Codon ◽  
Male Sterile ◽  
Specific Expression ◽  
Genome Wide ◽  
Tapetal Cells ◽  
Floral Abscission

Abstract Background:The Gretchen Hagen 3 (GH3) genes encode acyl acid amido synthetases, many of which have been shown to modulate the amount of active plant hormones or their precursors. GH3 genes, especially Group Ⅲ subgroup 6 GH3 genes, and their expression patterns in economically important kale-type Brassica oleracea have not been systematically identified. Results:As a first step to understand regulation and molecular functions of Group Ⅲ subgroup 6 GH3 genes, thirty-four GH3 genes including four subgroup 6 genes were identified In B. oleracea var. oleracea, using TO1000. Synteny found around subgroup 6 GH3 genes in TO1000 and Arabidopsis indicated that these genes are evolutionarily related. Although expression of four subgroup 6 GH3 genes in TO1000 is not induced by auxin, gibberellic acid, and jasmonic acid, the genes show different organ-dependent expression patterns. Only one TO1000 subgroup 6 GH3 gene, Bo2g011210, is expressed in anthers when microspores, polarized microspores, and bicellular pollens are present, similar to two out of four syntenic Arabidopsis subgroup 6 GH3 genes. Detailed analyses of promoter activities of Bo2g011210 further showed that Bo2g011210 is expressed in tapetal cells and pollens in anther, and also expressed in leaf primordia and floral abscission zones. Conclusions:Sixty-two base pair (bp) region (-340 ~ -279 bp upstream from start codon) and about 450 bp region (-1489 to -1017 bp) in Bo2g011210 promoter were found to be important for expressions in anther and expressions in leaf primordia and floral abscission zones, respectively. The identified anther-specific promoter region will be useful to develop male sterile transgenic Brassica plants.

scholarly journals Advances in Research and Application of Male Sterility in Brassica oleracea

Horticulturae ◽  
2020 ◽  
Vol 6 (4) ◽  
pp. 101
Author(s):  
Jialei Ji ◽  
Jianxin Huang ◽  
Limei Yang ◽  
Zhiyuan Fang ◽  
Yangyong Zhang ◽  
...  
Keyword(s):  
Male Sterility ◽  
Brassica Oleracea ◽  
Plant Reproduction ◽  
Male Sterile ◽  
Existing Problems ◽  
Recessive Genic Male Sterility ◽  
Cytological Characteristics ◽  
Yield Quality ◽  
Production Strategy ◽  
Heterosis Breeding

Brassica oleracea is an important vegetable species which belongs to the genus Brassica and the mustard family Brassicaceae Burnett. Strong heterosis in B. oleracea is displayed in yield, quality, disease resistance, and stress tolerance. Heterosis breeding is the main way to improve B. oleracea varieties. Male sterile mutants play an important role in the utilization of heterosis and the study of development and regulation in plant reproduction. In this paper, advances in the research and application of male sterility in B. oleracea were reviewed, including aspects of the genetics, cytological characteristics, discovery of genes related to male sterility, and application of male sterility in B. oleracea. Moreover, the main existing problems and prospect of male sterility application in B. oleracea were addressed and a new hybrids’ production strategy with recessive genic male sterility is introduced.

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