scholarly journals Environmental, genotypic, and ploidy effects on endopolyploidization within a genotype of Solanum phureja and its derivatives

Genome ◽  
1988 ◽  
Vol 30 (4) ◽  
pp. 506-510 ◽  
Author(s):  
H. R. Owen ◽  
R. E. Veilleux ◽  
D. Levy ◽  
D. L. Ochs
Keyword(s):  
Callus Culture ◽  
Dna Content ◽  
Nuclear Dna ◽  
Protoplast Culture ◽  
Nuclear Dna Content ◽  
Solanum Phureja ◽  
Time Variability ◽  
Flow Cytometric

Flow-cytometric analyses of DNA content were performed on chopped in vivo, in vitro, and protoplast-derived in vitro nuclei of Solanum phureja. An anther-derived monoploid genotype and a diploid and tetraploid clone, derived from callus culture of the monoploid genotype, were used to characterize the influence of in vivo and in vitro environment and explant ploidy level on the extent of endopolyploidization. In addition, protoplast-derived nuclei, from nine anther-derived monoploid genotypes, were examined for genotypic influences on endopolyploidization. DNA distributions of the anther-derived monoploid and callus-derived clones in vivo contained peaks corresponding to 1C, 2C, and 4C DNA levels. By comparison, diploid and tetraploid clones cultured in vitro did not contain 1C DNA peaks. Nuclear DNA content beyond the 4C level was not observed in any of the samples tested. The frequency of monoploid nuclei did not vary significantly among protoplast-derived nuclei from the monoploid genotypes; however, significant differences were detected between replications over time. Variability among the monoploid genotypes was shown for frequency of endoreplicated (4C) nuclei, indicating a genotypic influence on monoploid stability.Key words: potato, monoploid, anther culture, callus culture, protoplast culture, flow cytometry.

Nuclear DNA content and isozyme variation in relation to morphogenic potential of strawberry (Fragaria × ananassa) callus cultures

1991 ◽  
Vol 69 (2) ◽  
pp. 239-244 ◽  
Author(s):  
Narender S. Nehra ◽  
Kutty K. Kartha ◽  
Cecil Stushnoff
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Flow Cytometric Analysis ◽  
Nuclear Dna Content ◽  
Leaf Explants ◽  
Callus Cultures ◽  
Fragaria Ananassa ◽  
Flow Cytometric ◽  
Ploidy Changes

Callus cultures of strawberry cv. Redcoat (2n = 8x = 56) initiated from greenhouse and in vitro leaf explants were examined at various culture periods for morphogenic response, changes in nuclear DNA content, and isozyme banding patterns of four enzymes. The flow cytometric analysis of nuclear DNA content revealed the occurrence of polyploid and aneuploid changes as a function of ageing of callus cultures. The calli initiated from in vitro leaf explants were more prone to such changes than those initiated from greenhouse leaf explants. The in vitro morphogenic ability of callus cultures was affected by the ploidy changes, but the latter were not the only cause for loss in regeneration potential of long-term callus cultures. The isozyme phenotypes of esterase, phosphoglucomutase, phosphoglucoisomerase, and leucine aminopeptidase did not change with the chromosomal variation in callus cultures. Key words: strawberry, Fragaria × ananassa, callus culture, flow cytometry, nuclear DNA content, isozyme.

scholarly journals Reliable flow cytometric estimation of nuclear DNA content in coffee trees

Cytometry ◽  
1996 ◽  
Vol 24 (1) ◽  
pp. 32-38 ◽  
Author(s):  
Philippe Barre ◽  
Michel Noirot ◽  
Jacques Louarn ◽  
Christophe Duperray ◽  
Serge Hamon
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Nuclear Dna Content ◽  
Flow Cytometric

Flow cytometric analysis of nuclear DNA content in Musa

1999 ◽  
Vol 98 (8) ◽  
pp. 1344-1350 ◽  
Author(s):  
M. A. Lysák ◽  
M. Dolez˘elová ◽  
J. P. Horry ◽  
R. Swennen ◽  
J. Dolez˘el
Keyword(s):  
Dna Content ◽  
Nuclear Dna ◽  
Flow Cytometric Analysis ◽  
Nuclear Dna Content ◽  
Flow Cytometric
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