Root-colonization ability of antagonistic Streptomyces griseoviridis

1994 ◽  
Vol 163 (1) ◽  
pp. 77-83 ◽  
Author(s):  
Hanna Kortemaa ◽  
Hannu Rita ◽  
Kielo Haahtela ◽  
Aino Smolander
Keyword(s):  
Root Colonization ◽  
Streptomyces Griseoviridis ◽  
Colonization Ability ◽  
Antagonistic Streptomyces

scholarly journals Effect of soil-spraying time on root-colonization ability of antagonistic Streptomyces griseoviridis

1997 ◽  
Vol 6 (4) ◽  
pp. 341-348 ◽  
Author(s):  
Hanna Kortemaa ◽  
Kielo Haahtela ◽  
Aino Smolander
Keyword(s):  
Brassica Rapa ◽  
Daucus Carota ◽  
Soil Microbes ◽  
Root Colonization ◽  
Turnip Rape ◽  
Daucus Carota L ◽  
Streptomyces Griseoviridis ◽  
Colonization Ability ◽  
Antagonistic Streptomyces ◽  
Microbial Suspension

The root-colonization ability of Streptomyces griseoviridis Anderson et al. was tested on turnip rape (Brassica rapa subsp. oleifera DC.) and carrot (Daucus carota L.) by the sand-tube method. Nonsterile sand was sprayed with a microbial suspension immediately or 7 days after the seed had been sown. Results expressed as population frequencies and densities indicated that S. griseoviridis effectively colonizes the rhizosphere when the microbe is applied immediately after sowing but less effectively when it is applied 7 days later. Detection values of S. griseoviridis were higher for turnip rape than for carrot. In sterile sand, S. griseoviridis invaribly colonized the rhizosphere of turnip rape after each of the two applications. These findings indicate that S. griseoviridis can compete with indigenous soil microbes in the rhizosphere if it is sufficiently abundant in the soil before the seed emerges. If applied later, however, it competes rather poorly. In root-free nonsterile sand, S. griseoviridis dispersed and survived well.

scholarly journals Selection of Biocontrol Agents against Phytophthora Blight of Pepper and Its Root Colonization Ability

2010 ◽  
Vol 16 (2) ◽  
pp. 158-162 ◽  
Author(s):  
Li-Jing Zhang ◽  
Hong-Zhong Shi ◽  
Jing-Jing Wang ◽  
Shu-Xian Chang ◽  
Shun-Shan Shen
Keyword(s):  
Root Colonization ◽  
Biocontrol Agents ◽  
Phytophthora Blight ◽  
Colonization Ability ◽  
Selection Of

scholarly journals Whole Genome Sequencing and Root Colonization Studies Reveal Novel Insights in the Biocontrol Potential and Growth Promotion by Bacillus subtilis MBI 600 on Cucumber

2021 ◽  
Vol 11 ◽  
Author(s):  
Anastasios Samaras ◽  
Marios Nikolaidis ◽  
Maria Luisa Antequera-Gómez ◽  
Jesus Cámara-Almirón ◽  
Diego Romero ◽  
...  
Keyword(s):  
Plant Growth ◽  
Plant Growth Promotion ◽  
Root Colonization ◽  
Growth Promotion ◽  
Laser Scanning Microscopy ◽  
Main Role ◽  
Agricultural Practice ◽  
Sequencing Analysis ◽  
Colonization Ability ◽  
Cucumber Roots

Bacillus spp. MBI 600 is a gram-positive bacterium and is characterized as a PGPR strain involved in plant growth promotion and control of various plant pathogens which has recently been introduced into the agricultural practice. In this study we performed a Next Generation Sequencing analysis, to analyze the full genome of this microorganism and to characterize it taxonomically. Results showed that MBI 600 strain was phylogenetically close to other Bacillus spp. strains used as biocontrol agents and identified as B. subtilis. GOG analysis showed clusters contributed to secondary metabolites production such as fengycin and surfactin. In addition, various genes which annotated according to other plant-associated strains, showed that play a main role in nutrient availability from soil. The root colonization ability of MBI 600 strain was analyzed in vivo with a yellow fluorescence protein (yfp) tag. Confocal laser scanning microscopy of cucumber roots treated with yfp-tagged MBI 600 cells, revealed that the strain exhibits a strong colonization ability of cucumber roots, although it is affected significantly by the growth substrate of the roots. In vitro and in planta experiments with MBI 600 strain and F. oxysporum f.sp. radicis cucumerinum and P. aphanidernatum, showed a high control ability against these soilborne pathogens. Overall, our study demonstrates the effectiveness of MBI 600 in plant growth promotion and antagonism against different pathogens, highlighting the use of this microorganism as a biocontrol agent.

Biological Management of Fusarium Wilt in Chickpea (Cicer Arietinum L.) Caused by Fusarium oxysporum f. sp. ciceris

2019 ◽  
Author(s):  
P Murali Sankar ◽  
S Vanitha ◽  
A Kamalakannan ◽  
P Anantha Raju ◽  
P Jeyakumar
Keyword(s):  
Fusarium Wilt ◽  
Root Colonization ◽  
Wilt Disease ◽  
Field Conditions ◽  
Pseudomonas Chlororaphis ◽  
Cicer Arietinum L ◽  
Yield Parameters ◽  
Colonization Ability ◽  
Growth Promoting ◽  
Biological Management

In the present study, the three rhizobacterial strains (CPs3, CBs5 and Pf1) and fungal antagonists (CTs2 and Tv1) were evaluated against Fusarium wilt of chickpea under glasshouse and field conditions. Among all the treatments the strain CPs3 (Pseudomonas chlororaphis) has recorded highest germination (100%) and yield parameters viz., yield of 1194.4 kg/ ha with 13 (Number of pod bearing branches/plant), 32.3 (Pods/plant) and 33.0 g of 100 seeds weight with lowest incidence of wilt at 14.3% with disease reduction 80.7% (glasshouse) and 21.67% with 70.18% in the field conditions when compared to other biocontrol agents. The highest population of 8.2 x105 cfu/ g of soil and followed by Pf1 (Pseudomonas fluorescens) recorded 7.5 x105 cfu/g of soil. The strain CPs3 (Pseudomonas chlororaphis) had better growth promoting traits and management of the wilt disease in chickpea with superior root colonization ability.

scholarly journals Increased Uptake of Putrescine in the Rhizosphere Inhibits Competitive Root Colonization by Pseudomonas fluorescens Strain WCS365

2001 ◽  
Vol 14 (9) ◽  
pp. 1096-1104 ◽  
Author(s):  
Irene Kuiper ◽  
Guido V. Bloemberg ◽  
Sadaf Noreen ◽  
Jane E. Thomas-Oates ◽  
Ben J. J. Lugtenberg
Keyword(s):  
Growth Rate ◽  
Pseudomonas Fluorescens ◽  
Root Exudate ◽  
Root Colonization ◽  
Wild Type ◽  
Tomato Root ◽  
Mutant Strains ◽  
Colonization Ability ◽  
Flanking Regions ◽  
Layer Chromatography

Sequence analysis of the chromosomal Tn5lacZ flanking regions of the Pseudomonas fluorescens WCS365 competitive root colonization mutant PCL1206 showed that the Tn5lacZ is inserted between genes homologous to bioA and potF. The latter gene is the first gene of the potF1F2GHI operon, which codes for a putrescine transport system in Escherichia coli. The position of the Tn5lacZ suggests an effect on the expression of the pot operon. A mutation in the potF1 gene as constructed in PCL1270, however, had no effect on competitive root colonization. The rate of uptake of [1,4-14C]putrescine by cells of mutant PCL1206 appeared to be increased, whereas cells of strain PCL1270 were strongly impaired in the uptake of putrescine. Dansylation of tomato root exudate and subsequent thin-layer chromatography showed the presence of a component with the same Rf value as dansyl-putrescine, which was identified as dansyl-putrescine by mass spectrometric analyses. Other polyamines such as spermine and spermidine were not detected in the root exudate. Growth of mutant strains, either alone or in competition with the wild type, was tested in media containing putrescine, spermine, or spermidine as the sole nitrogen source. The results show that mutant PCL1206 is strongly impaired in growth on putrescine and slightly impaired on spermine and spermidine. The presence of the polyamines had a similar effect on the growth rate of strain PCL1270 in the presence of putrescine but a less severe effect in the presence of spermine and spermidine. We conclude that an increased rate of putrescine uptake has a bacteriostatic effect on Pseudomonas spp. cells. We have shown that putrescine is an important tomato root exudate component and that root-colonizing pseudomonads must carefully regulate their rate of uptake because increased uptake causes a decreased growth rate and, therefore, a decreased competitive colonization ability.

Distribution of Antagonistic Streptomyces griseoviridis in Rhizosphere and Nonrhizosphere Sand

1997 ◽  
Vol 145 (4) ◽  
pp. 137-143 ◽  
Author(s):  
H. Kortemaa ◽  
T. Pennanen ◽  
A. Smolander ◽  
K. Haahtela
Keyword(s):  
Streptomyces Griseoviridis ◽  
Antagonistic Streptomyces
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