Isolation of single-copy-sequence clones from a yeast artificial chromosome library of randomly-sheared Arabidopsis thaliana DNA

1990 ◽  
Vol 14 (4) ◽  
pp. 561-568 ◽  
Author(s):  
Eric R. Ward ◽  
George C. Jen
Keyword(s):  
Arabidopsis Thaliana ◽  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Single Copy ◽  
Yeast Artificial Chromosome Library ◽  
Single Copy Sequence ◽  
Copy Sequence

Characterization and evolution of a single-copy sequence from the human Y chromosome

1985 ◽  
Vol 5 (3) ◽  
pp. 576-581
Author(s):  
R D Burk ◽  
P Ma ◽  
K D Smith
Keyword(s):  
Y Chromosome ◽  
Primate Evolution ◽  
Single Copy ◽  
Homologous Sequence ◽  
Old World Monkeys ◽  
Chromosomal Distribution ◽  
Human Dna ◽  
Single Copy Sequence ◽  
Copy Sequence ◽  
Human Y Chromosome

To study the evolution and organization of DNA from the human Y chromosome, we constructed a recombinant library of human Y DNA by using a somatic cell hybrid in which the only cytologically detectable human chromosome is the Y. One recombinant (4B2) contained a 3.3-kilobase EcoRI single-copy fragment which was localized to the proximal portion of the Y long arm. Sequences homologous to this human DNA are present in male gorilla, chimpanzee, and orangutan DNAs but not in female ape DNAs. Under stringent hybridization conditions, the homologous sequence is either a single-copy or a low-order repeat in humans and in the apes. With relaxed hybridization conditions, this human Y probe detected several homologous DNA fragments which are all derived from the Y in that they occur in male DNAs from humans and the apes but not in female DNAs. In contrast, this probe hybridized to highly repeated sequences in both male and female DNAs from old world monkeys. Thus, sequences homologous to this probe underwent a change in copy number and chromosomal distribution during primate evolution.

scholarly journals An arbitrary single copy sequence VC64 [D1S86] detects a moderate frequency TaqI RFLP on chromosome 1

1989 ◽  
Vol 17 (10) ◽  
pp. 4009-4009
Author(s):  
Vera Verga ◽  
Maria Dos Santos ◽  
Isabelle Marques ◽  
Sue Povey ◽  
Michele Ramsay ◽  
...  
Keyword(s):  
Single Copy ◽  
Chromosome 1 ◽  
Moderate Frequency ◽  
Single Copy Sequence ◽  
Copy Sequence

Construction of a large-insert yeast artificial chromosome library of the rat genome

1997 ◽  
Vol 8 (4) ◽  
pp. 284-284 ◽  
Author(s):  
M. L. Haldi ◽  
P. Lim ◽  
K. Kaphingst ◽  
U. Akella ◽  
J. Whang ◽  
...  
Keyword(s):  
Yeast Artificial Chromosome ◽  
Artificial Chromosome ◽  
Yeast Artificial Chromosome Library ◽  
Rat Genome

scholarly journals Mapping expressed sequence tag sites on yeast artificial chromosome clones of Arabidopsis thaliana DNA.

1997 ◽  
Vol 7 (1) ◽  
pp. 1-9 ◽  
Author(s):  
F D Agyare ◽  
D A Lashkari ◽  
A Lagos ◽  
A F Namath ◽  
G Lagos ◽  
...  
Keyword(s):  
Arabidopsis Thaliana ◽  
Yeast Artificial Chromosome ◽  
Expressed Sequence Tag ◽  
Artificial Chromosome ◽  
Expressed Sequence

Genetic and contig map of a 2200-kb region encompassing 5.5 cM on chromosome 1 of Arabidopsis thaliana

Genome ◽  
1996 ◽  
Vol 39 (6) ◽  
pp. 1086-1092 ◽  
Author(s):  
Christian S. Hardtke ◽  
Thomas Berleth
Keyword(s):  
Arabidopsis Thaliana ◽  
Positional Cloning ◽  
Yeast Artificial Chromosome ◽  
Physical Map ◽  
Primary Root ◽  
Artificial Chromosome ◽  
Chromosome 1 ◽  
Rflp Markers ◽  
Caps Markers ◽  
Yac Contig

In the course of the isolation of the MONOPTEROS (MP) gene, required for primary root formation in Arabidopsis thaliana, a yeast artificial chromosome (YAC) contig encompassing approximately 2200 kilobases corresponding to 5.5 cM on the top arm of chromosome 1 was established. Forty-six YAC clones were characterized and 12 new restriction fragment length polymorphism (RFLP) markers are presented. Three new codominant amplified polymorphic sequence (CAPS) markers were generated that enabled high resolution genetic mapping and correlation of physical and genetic distances along the contig. The map contributes to the completion of a physical map of the Arabidopsis genome and should facilitate positional cloning of other genes in the region as well as studies on genome organization. We also present another set of 11 physically linked probes, as well as mapping data for additional RFLP markers within a broader interval of 10.4 cM. Key words : Arabidopsis, CAPS markers, MONOPTEROS gene, physical map, RFLP markers, YAC contig.

Single-copy sequence homology among the GC-richest isochores of the genomes from warm-blooded vertebrates

1994 ◽  
Vol 39 (4) ◽  
pp. 331-339 ◽  
Author(s):  
Simone Cacci� ◽  
Paolo Perani ◽  
Salvatore Saccone ◽  
Farida Kadi ◽  
Giorgio Bernardi
Keyword(s):  
Sequence Homology ◽  
Single Copy ◽  
Single Copy Sequence ◽  
Copy Sequence
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