Nucleotide sequence of a cDNA clone encoding a precursor to stearoyl-(acyl-carrier-protein) desaturase from spinach, Spinacia oleracea

1992 ◽  
Vol 19 (4) ◽  
pp. 711-713 ◽  
Author(s):  
Ikuo Nishida ◽  
Toshio Beppu ◽  
Tomoaki Matsuo ◽  
Norio Murata
Keyword(s):  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Spinacia Oleracea ◽  
Acyl Carrier Protein ◽  
Carrier Protein

scholarly journals The nucleotide sequence of a cDNA clone encoding acyl carrier protein (ACP) fromBrassica campestrisseeds

1987 ◽  
Vol 15 (17) ◽  
pp. 7197-7197 ◽  
Author(s):  
Ronald E. Rose ◽  
Cataline E. DeJesus ◽  
Susan L. Moylan ◽  
Neal P. Ridge ◽  
Donna E. Scherer ◽  
...  
Keyword(s):  
Nucleotide Sequence ◽  
Cdna Clone ◽  
Acyl Carrier Protein ◽  
Carrier Protein

scholarly journals Plant holo-(acyl carrier protein) synthase

1988 ◽  
Vol 252 (1) ◽  
pp. 39-45 ◽  
Author(s):  
S A Elhussein ◽  
J A Miernyk ◽  
J B Ohlrogge
Keyword(s):  
Spinacia Oleracea ◽  
Acyl Carrier Protein ◽  
Ricinus Communis ◽  
Plant Cells ◽  
Gel Permeation Chromatography ◽  
Carrier Protein ◽  
Improved Method ◽  
Ph Optimum ◽  
Spinach Leaf

1. An improved method was developed for the assay of plant holo-(acyl carrier protein) synthase activity, using Escherichia coli acyl-(acyl carrier protein) synthetase as a coupling enzyme. 2. Holo-(acyl carrier protein) synthase was partially purified from spinach (Spinacia oleracea) leaves by a combination of (NH4)2SO4 fractionation and anion-exchange and gel-permeation chromatography. 3. The partially purified enzyme had a pH optimum of 8.2 and Km values of 2 microM, 72 microM and 3 mM for apo-(acyl carrier protein), CoA and Mg2+ respectively. Synthase activity was inhibited in vitro by the reaction product 3′,5′-ADP. 4. Results from the fractionation of spinach leaf and developing castor-oil-seed (Ricinus communis) endosperm cells were consistent with a cytosolic localization of holo-(acyl carrier protein) synthase activity in plant cells.

scholarly journals Nucleotide Sequence of a Stearoyl-Acyl Carrier Protein Desaturase cDNA from Developing Seeds of Rice

1995 ◽  
Vol 108 (2) ◽  
pp. 845-846 ◽  
Author(s):  
H. Akagi ◽  
T. Baba ◽  
H. Shimada ◽  
T. Fujimura
Keyword(s):  
Nucleotide Sequence ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Developing Seeds

β-Ketoacyl-acyl carrier protein synthase III from pea (Pisum sativum L.): properties, inhibition by a novel thiolactomycin analogue and isolation of a cDNA clone encoding the enzyme

Planta ◽  
2003 ◽  
Vol 216 (5) ◽  
pp. 752-761 ◽  
Author(s):  
Lesley A. Jones ◽  
Andy M. Gane ◽  
Derek Herbert ◽  
David L. Willey ◽  
Andrew J. Rutter ◽  
...  
Keyword(s):  
Pisum Sativum ◽  
Cdna Clone ◽  
Acyl Carrier Protein ◽  
Carrier Protein ◽  
Pisum Sativum L
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