Spontaneous wheat/rye translocations from female meiotic products of hybrids between octoploid triticale and wheat

Euphytica ◽  
1995 ◽  
Vol 81 (3) ◽  
pp. 265-270 ◽  
Author(s):  
Y. B. Wang ◽  
H. Hu ◽  
J. W. Snape
Genome ◽  
2016 ◽  
Vol 59 (7) ◽  
pp. 485-492 ◽  
Author(s):  
Xuhui Ma ◽  
Qing Wang ◽  
Yanzhi Wang ◽  
Jieyun Ma ◽  
Nan Wu ◽  
...  

Chromosome engineering is an important approach for generating wheat germplasm. Efficient development of chromosome aberrations will facilitate the introgression and application of alien genes in wheat. In this study, zebularine, a DNA methylation transferase inhibitor, was successfully used to induce chromosome aberrations in the octoploid triticale cultivar Jinghui#1. Dry seeds were soaked in zebularine solutions (250, 500, and 750 μmol/L) for 24 h, and the 500 μmol/L treatment was tested in three additional treatment times, i.e., 12, 36, and 48 h. All treatments induced aberrations involving wheat and rye chromosomes. Of the 920 cells observed in 67 M1 plants, 340 (37.0%) carried 817 aberrations with an average of 0.89 aberrations per cell (range: 0–12). The aberrations included probable deletions, telosomes and acentric fragments (49.0%), large segmental translocations (28.9%), small segmental translocations (17.1%), intercalary translocations (2.6%), long chromosomes that could carry more than one centromere (2.0%), and ring chromosomes (0.5%). Of 510 M2 plants analyzed, 110 (21.6%) were found to carry stable aberrations. Such aberrations included 79 with varied rye chromosome numbers, 7 with wheat and rye chromosome translocations, 15 with possible rye telosomes/deletions, and 9 with complex aberrations involving variation in rye chromosome number and wheat–rye translocations. These indicated that aberrations induced by zebularine can be steadily transmitted, suggesting that zebularine is a new efficient agent for chromosome manipulation.


2013 ◽  
Vol 58 (3) ◽  
pp. 424-430 ◽  
Author(s):  
Malena Moiraghi ◽  
Leonardo Vanzetti ◽  
Laura Pflüger ◽  
Marcelo Helguera ◽  
Gabriela Teresa Pérez

Hereditas ◽  
2009 ◽  
Vol 49 (1-2) ◽  
pp. 78-90 ◽  
Author(s):  
A. MÜNTZING ◽  
N. J. HRISHI ◽  
C. TARKOWSKI
Keyword(s):  

Genome ◽  
2001 ◽  
Vol 44 (4) ◽  
pp. 509-516 ◽  
Author(s):  
Z X Shi ◽  
X M Chen ◽  
R F Line ◽  
H Leung ◽  
C R Wellings

The Yr9 gene, which confers resistance to stripe rust caused by Puccinia striiformis f.sp. tritici (P. s. tritici) and originated from rye, is present in many wheat cultivars. To develop molecular markers for Yr9, a Yr9 near-isogenic line, near-isogenic lines with nine other Yr genes, and the recurrent wheat parent 'Avocet Susceptible' were evaluated for resistance in the seedling stage to North American P. s. tritici races under controlled temperature in the greenhouse. The resistance gene analog polymorphism (RGAP) technique was used to identify molecular markers for Yr9. The BC7:F2 and BC7:F3 progeny, which were developed by backcrossing the Yr9 donor wheat cultivar Clement with 'Avocet Susceptible', were evaluated for resistance to stripe rust races. Genomic DNA was extracted from 203 BC7:F2 plants and used for cosegregation analysis. Of 16 RGAP markers confirmed by cosegregation analysis, 4 were coincident with Yr9 and 12 were closely linked to Yr9 with a genetic distance ranging from 1 to 18 cM. Analyses of nulli-tetrasomic 'Chinese Spring' lines with the codominant RGAP marker Xwgp13 confirmed that the markers and Yr9 were located on chromosome 1B. Six wheat cultivars reported to have 1B/1R wheat-rye translocations and, presumably, Yr9, and two rye cultivars were inoculated with four races of P. s. tritici and tested with 9 of the 16 RGAP markers. Results of these tests indicate that 'Clement', 'Aurora', 'Lovrin 10', 'Lovrin 13', and 'Riebesel 47/51' have Yr9 and that 'Weique' does not have Yr9. The genetic information and molecular markers obtained from this study should be useful in cloning Yr9, in identifying germplasm that may have Yr9, and in using marker-assisted selection for combining Yr9 with other stripe rust resistance genes.Key words: molecular markers, Puccinia striiformis f.sp. tritici, resistance gene analog polymorphism, Triticum aestivum.


Hereditas ◽  
2009 ◽  
Vol 80 (1) ◽  
pp. 121-130 ◽  
Author(s):  
ANNA WEIMARCK
Keyword(s):  

2018 ◽  
Vol 0 (113) ◽  
pp. 8-17 ◽  
Author(s):  
О. М. Бакуменко ◽  
В. А. Власенко

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