Structure and light-regulated expression of the gsa gene encoding the chlorophyll biosynthetic enzyme, glutamate 1-semialdehyde aminotransferase, in Chlamydomonas reinhardtii

1994 ◽  
Vol 24 (4) ◽  
pp. 617-629 ◽  
Author(s):  
Gail L. Matters ◽  
Samuel I. Beale
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biosynthetic Enzyme ◽  
Gene Encoding ◽  
Regulated Expression

The Chlamydomonas reinhardtii gtr Gene Encoding the Tetrapyrrole Biosynthetic Enzyme Glutamyl-tRNA Reductase: Structure of the Gene and Properties of the Expressed Enzyme

2005 ◽  
Vol 58 (5) ◽  
pp. 643-658 ◽  
Author(s):  
Alaka Srivastava ◽  
Vanessa Lake ◽  
Luiza A. Nogaj ◽  
Sandra M. Mayer ◽  
Robert D. Willows ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biosynthetic Enzyme ◽  
Gene Encoding

scholarly journals The Schizosaccharomyces pombe cho1+ Gene Encodes a Phospholipid Methyltransferase

Genetics ◽  
1998 ◽  
Vol 150 (2) ◽  
pp. 553-562
Author(s):  
Margaret I Kanipes ◽  
John E Hill ◽  
Susan A Henry
Keyword(s):  
Saccharomyces Cerevisiae ◽  
Sequence Analysis ◽  
Schizosaccharomyces Pombe ◽  
Gene Disruption ◽  
Structure And Function ◽  
Biosynthetic Enzyme ◽  
Gene Encoding ◽  
Complementation Groups ◽  
Eukaryotic Organisms ◽  
And Function

Abstract The isolation of mutants of Schizosaccharomyces pombe defective in the synthesis of phosphatidylcholine via the methylation of phosphatidylethanolamine is reported. These mutants are choline auxotrophs and fall into two unlinked complementation groups, cho1 and cho2. We also report the analysis of the cho1+ gene, the first structural gene encoding a phospholipid biosynthetic enzyme from S. pombe to be cloned and characterized. The cho1+ gene disruption mutant (cho1Δ) is viable if choline is supplied and resembles the cho1 mutants isolated after mutagenesis. Sequence analysis of the cho1+ gene indicates that it encodes a protein closely related to phospholipid methyltransferases from Saccharomyces cerevisiae and rat. Phospholipid methyltransferases encoded by a rat liver cDNA and the S. cerevisiae OPI3 gene are both able to complement the choline auxotrophy of the S. pombe cho1 mutants. These results suggest that both the structure and function of the phospholipid N-methyltransferases are broadly conserved among eukaryotic organisms.

Structure and expression of the gene encoding the periplasmic arylsulfatase of Chlamydomonas reinhardtii

1989 ◽  
Vol 218 (2) ◽  
pp. 229-239 ◽  
Author(s):  
Eugenio L. de Hostos ◽  
James Schilling ◽  
Arthur R. Grossman
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Gene Encoding

scholarly journals Successful Transient Expression of Cas9 and Single Guide RNA Genes in Chlamydomonas reinhardtii

2014 ◽  
Vol 13 (11) ◽  
pp. 1465-1469 ◽  
Author(s):  
Wenzhi Jiang ◽  
Andrew J. Brueggeman ◽  
Kempton M. Horken ◽  
Thomas M. Plucinak ◽  
Donald P. Weeks
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Transient Expression ◽  
Gene Knockout ◽  
Nuclease Activity ◽  
Gene Replacement ◽  
Target Site ◽  
Gene Encoding ◽  
Content Type ◽  
Guide Rna ◽  
Eukaryotic Organisms

ABSTRACT The clustered regularly interspaced short palindromic repeat (CRISPR)/Cas9 system has become a powerful and precise tool for targeted gene modification (e.g., gene knockout and gene replacement) in numerous eukaryotic organisms. Initial attempts to apply this technology to a model, the single-cell alga, Chlamydomonas reinhardtii , failed to yield cells containing edited genes. To determine if the Cas9 and single guide RNA (sgRNA) genes were functional in C. reinhardtii , we tested the ability of a codon-optimized Cas9 gene along with one of four different sgRNAs to cause targeted gene disruption during a 24-h period immediately following transformation. All three exogenously supplied gene targets as well as the endogenous FKB12 (rapamycin sensitivity) gene of C. reinhardtii displayed distinct Cas9/sgRNA-mediated target site modifications as determined by DNA sequencing of cloned PCR amplicons of the target site region. Success in transient expression of Cas9 and sgRNA genes contrasted with the recovery of only a single rapamycin-resistant colony bearing an appropriately modified FKB12 target site in 16 independent transformation experiments involving >10 9 cells. Failure to recover transformants with intact or expressed Cas9 genes following transformation with the Cas9 gene alone (or even with a gene encoding a Cas9 lacking nuclease activity) provided strong suggestive evidence for Cas9 toxicity when Cas9 is produced constitutively in C. reinhardtii . The present results provide compelling evidence that Cas9 and sgRNA genes function properly in C. reinhardtii to cause targeted gene modifications and point to the need for a focus on development of methods to properly stem Cas9 production and/or activity following gene editing.

Constitutive and regulated expression of the mouse Dinb (Polκ) gene encoding DNA polymerase kappa

DNA Repair ◽  
2003 ◽  
Vol 2 (1) ◽  
pp. 91-106 ◽  
Author(s):  
Susana Velasco-Miguel ◽  
James A. Richardson ◽  
Valerie L. Gerlach ◽  
Wayne C. Lai ◽  
Tianshu Gao ◽  
...  
Keyword(s):  
Dna Polymerase ◽  
Gene Encoding ◽  
Regulated Expression

scholarly journals Regulated expression and RNA processing of transcripts from the Srp20 splicing factor gene during the cell cycle.

1997 ◽  
Vol 17 (6) ◽  
pp. 3116-3124 ◽  
Author(s):  
H Jumaa ◽  
J L Guénet ◽  
P J Nielsen
Keyword(s):  
Cell Cycle ◽  
Rna Binding ◽  
Cpg Island ◽  
Standard Form ◽  
Alternative Exon ◽  
Chromosome 17 ◽  
Alternative Form ◽  
Splicing Factors ◽  
Gene Encoding ◽  
Regulated Expression

Eukaryotic splicing factors belonging to the SR family are essential splicing factors consisting of an N-terminal RNA-binding region and a C-terminal RS domain. They are believed to be involved in alternative splicing of numerous transcripts because their expression levels can influence splice site selection. We have characterized the structure and transcriptional regulation of the gene for the smallest member of the SR family, SRp20 (previously called X16). The mouse gene encoding SRp20, termed Srp20, consists of one alternative exon and six constitutive exons and was mapped to a 2-centimorgan interval on chromosome 17. When cells are transfected with SRp20 genomic DNA, both standard and alternatively spliced transcripts and corresponding proteins are produced. Interestingly, in starved (G0) cells, the amount of SRp20 mRNA containing the alternative exon is large, whereas the amount of the standard SRp20 mRNA without the alternative exon is small. When starved cells are stimulated with serum, the alternative form is lost and the standard form is induced. These results suggest that splicing could be regulated during the cell cycle and that this could be, at least in part, due to regulated expression of SR proteins. Consistent with this, experiments with synchronized cells showed an induction of SRp20 transcripts in late G1 or early S. We have also characterized the promoter of SRp20. It lies within a GC-rich CpG island and contains two consensus binding sites for E2F, a transcription factor thought to be involved in regulating the cell cycle. These motifs may be functional since reporter constructs with the SRp20 promoter can be stimulated by cotransfection with E2F expression plasmids.

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