Structure and expression of the Chlamydomonas reinhardtii alad gene encoding the chlorophyll biosynthetic enzyme, ?-aminolevulinic acid dehydratase (porphobilinogen synthase)

1995 ◽  
Vol 27 (3) ◽  
pp. 607-617 ◽  
Author(s):  
Gail L. Matters ◽  
Samuel I. Beale
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Aminolevulinic Acid ◽  
Biosynthetic Enzyme ◽  
Gene Encoding ◽  
Acid Dehydratase ◽  
Porphobilinogen Synthase

The Chlamydomonas reinhardtii gtr Gene Encoding the Tetrapyrrole Biosynthetic Enzyme Glutamyl-tRNA Reductase: Structure of the Gene and Properties of the Expressed Enzyme

2005 ◽  
Vol 58 (5) ◽  
pp. 643-658 ◽  
Author(s):  
Alaka Srivastava ◽  
Vanessa Lake ◽  
Luiza A. Nogaj ◽  
Sandra M. Mayer ◽  
Robert D. Willows ◽  
...  
Keyword(s):  
Chlamydomonas Reinhardtii ◽  
Biosynthetic Enzyme ◽  
Gene Encoding

The specific activities of peroxidase and aminolevulinic acid dehydratase during the growth cycle of peanut suspension culture

1976 ◽  
Vol 54 (18) ◽  
pp. 2089-2094 ◽  
Author(s):  
V. C. Kossatz ◽  
R. B. van Huystee
Keyword(s):  
Suspension Culture ◽  
Protein Content ◽  
Aminolevulinic Acid ◽  
Specific Activity ◽  
Growth Cycle ◽  
Acid Dehydratase ◽  
Green Tissue ◽  
Porphobilinogen Synthase ◽  
Dehydratase Activity ◽  
Ala Dehydratase

Peroxidase (EC 1.11.1.7) enzyme units that were recovered by sequential extraction at low and high ionic strength buffer pH 7 from a peanut suspension culture at various intervals of cell growth appeared to increase with culture time. In particular, at the end of a 14-day growth cycle, peroxidase specific activity rose while protein content and the specific activity of porphobilinogen synthase (aminolevulinic acid, dehydratase EC 4.2.1.24) declined. The decrease of the protein content could probably be accounted for by the 95% decrease of protein synthesis at the end of the growth phase. The highest specific activity of aminolevulinic acid dehydratase (ALA dehydratase) appeared to be associated with organelles which could be precipitated at 500 × g and this specific activity appeared to be unaffected by the stage of growth of these cells. It is suggested that the ALA dehydratase activity in nonchlorophyllous peanut cells, which is as high as that found in green tissue, may play a function in the biosynthesis of the hemoprotein peroxidase.

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