Molecular diversity and plant disease resistance: An electrophoretic comparison of near-isogenic lines of wilt-resistant or -susceptible Pisum sativum L. cv. William Massey

Euphytica ◽  
1982 ◽  
Vol 31 (2) ◽  
pp. 341-348 ◽  
Author(s):  
J. S. Hunt ◽  
M. F. Barnes
Keyword(s):  
Disease Resistance ◽  
Pisum Sativum ◽  
Plant Disease Resistance ◽  
Molecular Diversity ◽  
Plant Disease ◽  
Near Isogenic Lines ◽  
Isogenic Lines ◽  
Pisum Sativum L

COMPARISON OF NEAR-ISOGENIC LEAFED, LEAFLESS, SEMI-LEAFLESS, AND REDUCED STIPULE LINES OF PEAS FOR YIELD AND ASSOCIATED TRAITS

1981 ◽  
Vol 61 (2) ◽  
pp. 463-465 ◽  
Author(s):  
G. LAFOND ◽  
L. E. EVANS ◽  
S. T. ALI-KHAN
Keyword(s):  
Pisum Sativum ◽  
Individual Plant ◽  
Near Isogenic Lines ◽  
Normal Type ◽  
Consistent Effect ◽  
Isogenic Lines ◽  
Pisum Sativum L ◽  
Effect On Yield ◽  
Plant Basis

Yield and associated traits on an individual plant basis were studied in near-isogenic lines of Century and Trapper peas (Pisum sativum L.). The lines were near-isogenic for normal leaves (++++), semi-leafless (afaf++ — leaflets replaced by tendrils), reduced stipules (++stst), and leafless (afafstst — tendrils and reduced stipules). The data indicate that the genotypic background greatly influences the performance of the semi-leafless and leafless mutants. In the Century background, the semi-leafless type yielded similar to the leafed type. However, the yield of the leafless type was significantly reduced. In the Trapper background, the semi-leafless phenotype was markedly inferior to the normal type. These mutations had no consistent effect on yield or associated traits.

Construction and characterization of two bacterial artificial chromosome libraries of pea (Pisum sativum L.) for the isolation of economically important genes

Genome ◽  
2007 ◽  
Vol 50 (9) ◽  
pp. 871-875 ◽  
Author(s):  
C.J. Coyne ◽  
M.T. McClendon ◽  
J.G. Walling ◽  
G.M. Timmerman-Vaughan ◽  
S. Murray ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Bacterial Artificial Chromosome ◽  
Pisum Sativum ◽  
Plant Disease Resistance ◽  
Copy Number ◽  
Artificial Chromosome ◽  
Pisum Sativum L ◽  
A Genome ◽  
Low Copy Number ◽  
Bac Libraries

Pea ( Pisum sativum L.) has a genome of about 4 Gb that appears to share conserved synteny with model legumes having genomes of 0.2–0.4 Gb despite extensive intergenic expansion. Pea plant inventory (PI) accession 269818 has been used to introgress genetic diversity into the cultivated germplasm pool. The aim here was to develop pea bacterial artificial chromosome (BAC) libraries that would enable the isolation of genes involved in plant disease resistance or control of economically important traits. The BAC libraries encompassed about 3.2 haploid genome equivalents consisting of partially HindIII-digested DNA fragments with a mean size of 105 kb that were inserted in 1 of 2 vectors. The low-copy oriT-based T-DNA vector (pCLD04541) library contained 55 680 clones. The single-copy oriS-based vector (pIndigoBAC-5) library contained 65 280 clones. Colony hybridization of a universal chloroplast probe indicated that about 1% of clones in the libraries were of chloroplast origin. The presence of about 0.1% empty vectors was inferred by white/blue colony plate counts. The usefulness of the libraries was tested by 2 replicated methods. First, high-density filters were probed with low copy number sequences. Second, BAC plate-pool DNA was used successfully to PCR amplify 7 of 9 published pea resistance gene analogs (RGAs) and several other low copy number pea sequences. Individual BAC clones encoding specific sequences were identified. Therefore, the HindIII BAC libraries of pea, based on germplasm accession PI 269818, will be useful for the isolation of genes underlying disease resistance and other economically important traits.

Role of transporters in plant disease resistance

2021 ◽  
Vol 171 (4) ◽  
pp. 849-867
Author(s):  
Basavantraya N. Devanna ◽  
Rajdeep Jaswal ◽  
Pankaj Kumar Singh ◽  
Ritu Kapoor ◽  
Priyanka Jain ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Plant Disease Resistance ◽  
Plant Disease
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