Characterization of a cDNA encoding the protein moiety of a putative arabinogalactan protein from Lycopersicon esculentum

1995 ◽  
Vol 28 (2) ◽  
pp. 347-352 ◽  
Author(s):  
Barry J. Pogson ◽  
Chris Davies
Keyword(s):  
Lycopersicon Esculentum ◽  
Arabinogalactan Protein ◽  
Protein Moiety

Characterization of glycopeptides derived from the low-density lipoprotein of hen's egg yolk

1968 ◽  
Vol 46 (8) ◽  
pp. 983-988 ◽  
Author(s):  
J. Z. Augustyniak ◽  
W. G. Martin
Keyword(s):  
Low Density Lipoprotein ◽  
Density Lipoprotein ◽  
Egg Yolk ◽  
Low Density ◽  
Amide Nitrogen ◽  
Acetylneuraminic Acid ◽  
Hen’S Egg ◽  
Terminal Amino ◽  
Protein Moiety

Two glycopeptides (A and B) were isolated from pronase-digested vitellenin, the protein moiety of the low-density lipoprotein of hen's egg yolk. Aspartic acid was the only N-terminal amino acid of both glycopeptides but only A contained N-acetylneuraminic acid. A contained 55% hexose (mannose), 14% hexosamine, 12% N-acetylneuraminic acid, 0.71% amide nitrogen, and its molecular weight was 2.3 × 103. The corresponding values for B were 64, 17, 0.0, 0.75, and 2.0 × 103. Chemical analyses showed that B (and probably A) occurs in vitellenin with the heteropolysaccharide group bound N-glycosidically via the β-amide group of an asparaginyl residue. The indicated structure is R∙(NH)Asp∙Thr∙Ser∙(Ala, Gly, Val)∙Ile, where R, the heteropolysaccharide group, contains 2 hexosamine and 8 hexose residues.

scholarly journals Characterization of the hydroxyproline-rich protein core of an arabinogalactan-protein secreted from suspension-cultured Lolium multiflorum (Italian ryegrass) endosperm cells

1989 ◽  
Vol 264 (3) ◽  
pp. 857-862 ◽  
Author(s):  
P A Gleeson ◽  
M McNamara ◽  
R E H Wettenhall ◽  
B A Stone ◽  
G B Fincher
Keyword(s):  
Polyclonal Antibodies ◽  
Lolium Multiflorum ◽  
Italian Ryegrass ◽  
Arabinogalactan Protein ◽  
Myeloma Protein ◽  
Protein Core ◽  
Liquid Suspension ◽  
Peptide Component ◽  
Immunodominant Epitopes

An arabinogalactan-protein (AGP) purified from the filtrate of liquid-suspension-cultured Italian-ryegrass (Lolium multiflorum) endosperm cells by affinity chromatography on myeloma protein J539-Sepharose was deglycosylated with trifluoromethanesulphonic acid to remove polysaccharide chains that are covalently associated with hydroxyproline residues in the peptide component of the proteoglycan. The protein core, which accounts for less than 10% (w/w) of the intact proteoglycan, was purified by h.p.l.c. It has an apparent Mr of 35,000, but reacts very poorly with both Coomassie Brilliant Blue R and silver stains. Amino-acid-sequence analysis of the N-terminus of the h.p.l.c.-purified protein core and of tryptic peptides generated from the unpurified protein reveals a high content of hydroxyproline and alanine. These are sometimes arranged in short (Ala-Hyp) repeat sequences of up to six residues. Polyclonal antibodies raised against the protein core do not cross-react with native AGP, the synthetic peptide (Ala-Hyp)4, poly-L-hydroxyproline or poly-L-proline. The results suggest that the polysaccharide chains in the native AGP render the protein core of the proteoglycan inaccessible to the antibodies and that the immunodominant epitopes include domains of the protein other than those rich in Ala-Hyp repeating units.

Isolation and characterization of nitrate reductase-deficient mutants in tomato (Lycopersicon esculentum Mill.)

1991 ◽  
Vol 227 (3) ◽  
pp. 458-464 ◽  
Author(s):  
Herman C. H. Schoenmakers ◽  
Maarten Koornneef ◽  
Sjefke J. H. M. Alefs ◽  
Wendy F. M. Gerrits ◽  
Dianne van der Kop ◽  
...  
Keyword(s):  
Lycopersicon Esculentum ◽  
Nitrate Reductase ◽  
Lycopersicon Esculentum Mill ◽  
Isolation And Characterization

Biosynthesis of Arabinogalactan-Protein in Lolium multiflorum Ryegrass Endosperm Cells. I. Hydroxylation of Peptidyl Proline

1981 ◽  
Vol 8 (2) ◽  
pp. 121
Author(s):  
PC Pollard ◽  
GB Fincher
Keyword(s):  
Lolium Multiflorum ◽  
Total Radioactivity ◽  
Ferrous Ion ◽  
Arabinogalactan Protein ◽  
Trichloracetic Acid ◽  
Radioactive Label ◽  
Insoluble Material ◽  
Imino Acid ◽  
Proline Hydroxylation ◽  
Protein Moiety

Suspension-cultured endosperm cells from L. multiflorum secrete into the medium an arabinogalactan-protein in which the protein moiety is rich in hydroxyproline. When cells are grown in the presence of proline labelled with 14C or 3H, the imino acid is rapidly removed from the medium and radio- activity can subsequently be detected in extracellular trichloracetic acid-soluble, ethanol-insoluble material. In this fraction, which contains the arabinogalactan-protein and other polysaccharides, radioactive label is distributed between proline and hydroxyproline. α,α'-Dipyridyl, a chelator of ferrous ion, has no effect on the total radioactivity secreted but markedly alters the distribution of radioactivity in favour of peptidyl proline. Although this inhibition of peptidyl proline hydroxylation can be reversed by ferrous or zinc ions, it is not possible to conclude that ferrous ion, which is required for hydroxylation in other systems, participates specifically in the reaction in ryegrass endosperm cells. Concomitant with the inhibition of proline hydroxylation, α,α'-dipyridyl suppresses the biosynthesis or secretion of extracellular arabinogalactan-protein and arabinoxylan.

Isolation and characterization of rad51 orthologs from Coprinus cinereus and Lycopersicon esculentum, and phylogenetic analysis of eukaryotic recA homologs

1997 ◽  
Vol 31 (2) ◽  
pp. 144-157 ◽  
Author(s):  
Natalie Yeager Stassen ◽  
J. M. Logsdon Jr. ◽  
G. J. Vora ◽  
Hildo H. Offenberg ◽  
Jeffrey D. Palmer ◽  
...  
Keyword(s):  
Phylogenetic Analysis ◽  
Lycopersicon Esculentum ◽  
Coprinus Cinereus ◽  
Isolation And Characterization

scholarly journals Characterization of the Arabinogalactan Protein 31 (AGP31) ofArabidopsis thaliana

2012 ◽  
Vol 287 (12) ◽  
pp. 9623-9632 ◽  
Author(s):  
May Hijazi ◽  
Jessica Durand ◽  
Carole Pichereaux ◽  
Frédéric Pont ◽  
Elisabeth Jamet ◽  
...  
Keyword(s):  
Arabinogalactan Protein ◽  
Ofarabidopsis Thaliana

scholarly journals Isolation and Characterization of Arabinogalactan-protein from the Fruit of Lycium chinense Mill.

2000 ◽  
Vol 47 (2) ◽  
pp. 155-161 ◽  
Author(s):  
Xiaoming Qin ◽  
Ryo Yamauchi ◽  
Koichi Aizawa ◽  
Takahiro Inakuma ◽  
Koji Kato
Keyword(s):  
Arabinogalactan Protein ◽  
Lycium Chinense ◽  
Isolation And Characterization
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