Subunit interaction enhances enzyme activity and stability of sweet potato cytosolic Cu/Zn-superoxide dismutase purified by a His-tagged recombinant protein method

1995 ◽  
Vol 28 (2) ◽  
pp. 303-311 ◽  
Author(s):  
Chi-Tsai Lin ◽  
Ming-Tse Lin ◽  
Yu-Ting Chen ◽  
Jei-Fu Shaw
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Recombinant Protein ◽  
Sweet Potato ◽  
Subunit Interaction

Amino acids flanking the central core of Cu,Zn superoxide dismutase are important in retaining enzyme activity after autoclaving

2016 ◽  
Vol 34 (3) ◽  
pp. 475-485 ◽  
Author(s):  
Arun Kumar ◽  
Vinay Randhawa ◽  
Vishal Acharya ◽  
Kashmir Singh ◽  
Sanjay Kumar
Keyword(s):  
Amino Acids ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Central Core

scholarly journals The Gene Structure of Cu/Zn-Superoxide Dismutase from Sweet Potato

1995 ◽  
Vol 108 (2) ◽  
pp. 827-828 ◽  
Author(s):  
C. T. Lin ◽  
M. T. Lin ◽  
Y. T. Chen ◽  
J. F. Shaw
Keyword(s):  
Superoxide Dismutase ◽  
Sweet Potato ◽  
Gene Structure

Stressed Response of Submerged Elodea nattalii under Eutrophication Growth Conditions

2012 ◽  
Vol 610-613 ◽  
pp. 229-234
Author(s):  
Han Feng Xiong ◽  
Qi Ling Tan
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Aquatic Plants ◽  
Nutrient Concentration ◽  
Growth Conditions ◽  
Antioxidant Enzyme Activity ◽  
Metabolic Responses ◽  
Concentration Increase ◽  
Time Period ◽  
Mda Content

Eutrophication in water bodies affects the growth of aquatic plants. In this study, we conducted static experiments to better understand the metabolic responses of Elodea nattalii under eutrophication conditions. The nitrogen (N) and phosphorous (P) levels in tissue, malondiadehyde (MDA), and activities of three antioxidases (peroxidase (POD), superoxide dismutase (SOD), and catalase (CAT)) of Elodea nattalii cultured at different nutrient levels (oligotropher, mesotropher, eutropher, and hypertrophics) were investigated. The results showed that with nutrient concentration increase, N and P contents of Elodea nattalii increased. The MDA content improved with nutrient increase. the POD activity initially increased and later decreased. CAT and SOD activities decreased during the experimental time period. These finding suggested that changes in stem the increase of antioxidant enzyme activity can be served as the indicators of the response of Elodea nattalii to eutrophication conditions.

scholarly journals Вплив хлориду кадмію на стан антиоксидантної системи у печінці щурів

2013 ◽  
pp. 102-103
Author(s):  
Б. В. Гутий
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Glutathione Peroxidase ◽  
Antioxidant System ◽  
Cadmium Chloride

Розкрито особливості антиоксидантної системиорганізму щурів за хронічного кадмієвого токсикозу.Встановлено, що хлорид кадмію у токсичній дозісприяє зниженню активності ферментної й нефер-ментної системи антиоксидантного захисту, на щовказує зниження ферментів глутатіонпероксидази,глутатіонредуктази, супероксиддисмутази, катала-зи та відновленого глутатіону у печінці щурів. Ре-зультати досліджень вказують на те, що хронічнийкадмієвий токсикоз призводить до посиленої акти-вації процесів ліпопероксидації. The features of the antioxidant system of rats with chronic cadmium toxicosiare disclosed. It wasresearched that cadmium chloride in toxic doses reduces enzyme activity of antioxidant system, asindicated by the decrease in enzyme glutathione peroxidase, hlutationreduktazy, superoxide dismutase,catalase and restored glutathione in the liver and blood of rats. The results indicate that chroniccadmium toxicosis leads to enhanced activation of lipid peroxidation.

scholarly journals THE CORRELATION BETWEEN FAT INTAKE AND PLASMA SUPEROXIDE DISMUTASE ACTIVITY WITH TELOMERE LENGTH OF MINANGKABAU ETHNICITY MEN, IN WEST SUMATERA, INDONESIA

2019 ◽  
pp. 305-309
Author(s):  
AFRIWARDI AFRIWARDI ◽  
DELMI SULASTRI ◽  
YUNIAR LESTARI ◽  
DESMAWATI DESMAWATI ◽  
PRIMA MINERVA
Keyword(s):  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Fatty Acid ◽  
Telomere Length ◽  
Saturated Fatty Acids ◽  
Cross Sectional Study ◽  
Fat Intake ◽  
Sod Activity ◽  
Cross Sectional ◽  
Total Fat

Objective: The purpose of this research is to investigate the correlation between fat intake and plasma superoxide dismutase (SOD) enzyme activity with telomere length of Minangkabau ethnic men in West Sumatera, Indonesia. Materials and Methods: This cross-sectional study was conducted in Padang City in 2016, using a sample of 107 Minangkabau ethnic men, aged between 40 and 50 years. The total fat, saturated fatty acids (SFA), monounsaturated fatty acid (MUFA), and polyunsaturated fatty acid (PUFA) intake were collected using a food frequency questionnaire, and blood sample analysis to measure telomere length and plasma SOD activity. Results: This research indicated that an average telomere length was 584.59±305.92 bp, fat intake consists of total fat was 27.01±6.68% calorie, SFA was 24,7510.07% calorie, MUFA was 10.24±5.41% calorie, and PUFA was 7.35±3.54% calorie. Average of plasma SOD activity was 5.85±3.41 u/mL. There were no correlations between fat intake (total fat, SFA, MUFA, and PUFA) with telomere length (p>0.05), but there was a significant positive correlation between plasma SOD enzyme activity with telomere length (r=−0.202, p=0.037). Conclusion: Fat intake within normal limits does not correlate with telomere length, and plasma SOD activity is a risk factor for telomere shortening in ethnic Minangkabau men.

scholarly journals Pengaruh pemberian ubi jalar ungu (Ipomoea batatas L.Poir) terhadap kadar superoksida dismutase (SOD) tikus wistar jantan (Rattus Norvegicus) yang dipapar asap rokok

2020 ◽  
Vol 8 (1) ◽  
pp. 45
Author(s):  
Alfreda Sabrina Widyanti ◽  
Martha Ardiaria ◽  
Nurmasari Widyastuti
Keyword(s):  
Superoxide Dismutase ◽  
Sweet Potato ◽  
Cigarette Smoke ◽  
Ipomoea Batatas ◽  
Smoke Exposure ◽  
Cigarette Smoke Exposure ◽  
Control Group ◽  
Standard Diet ◽  
Control Group Design ◽  
Significant Difference

Background: Cigarette smoke exposure is one of the source of free radicals that causes oxidative stress and decreases superoxide dismutase (SOD) level. Purple fleshed sweet potato is a type of food that contains antioxidants to neutralize oxidative stress.Objectives: To study the effect of purple fleshed sweet potato on superoxide dismutase (sod) level on rats exposed to cigarette smoke.Methods: This was a true experimental study with a post-test randomized control group design. The rats were randomized into 4 groups (6 rats in each group). The negative control group (K-) was treated with standard diet; the positive control group (K+) was treated with cigarette smoke exposure and standard diet; the treatment 1 (P1) group was treated with standard diet and purple fleshed sweet potato with the dose of 8 g / 200 g bw/day, and the treatment 2 (P2) group was treated with cigarette smoke exposure and purple fleshed sweet potato with the dose of 8 g /200 g bw/day.Results: There was a significant difference of SOD levels in each group (p=0.00) except between group K- and P1. Giving purple fleshed sweet potatoes increased SOD levels as much as 85.81±4.59 (P1). The K+ group had the lowest SOD level 22.34±3.98. The SOD level for K- group and P2 group was 82.27±4.59 and 67.73±6.68 respectively.Conclusion: The highest SOD level is on the treatment 1 group which is administered with purple fleshed sweet potato.

scholarly journals Isolation and characterization of a rat cDNA clone encoding a secreted superoxide dismutase reveals the epididymis to be a major site of its expression

1993 ◽  
Vol 293 (1) ◽  
pp. 21-25 ◽  
Author(s):  
A C F Perry ◽  
R Jones ◽  
L Hall
Keyword(s):  
Lipid Peroxidation ◽  
Superoxide Dismutase ◽  
Enzyme Activity ◽  
Sequence Analysis ◽  
Cdna Clone ◽  
Northern Blot ◽  
Northern Blot Analysis ◽  
Major Site ◽  
Isolation And Characterization

Superoxide dismutase (SOD) plays a key role in combating loss of fertility of spermatozoa due to lipid peroxidation. Here we report the sequence of a cDNA encoding a secreted form of SOD isolated from a rat epididymal library. Northern-blot analysis indicates that the corresponding transcript is expressed principally in the cauda region of the epididymis, consistent with the high levels of SOD enzyme activity found in cauda-epididymidal plasma. Much lower levels of an identically sized transcript exist in all tissues examined, including placenta. PCR and subsequent sequence analysis of rat placental SOD strongly suggest that it is identical in sequence with epididymal SOD.

A highly stable Cu/Zn superoxide dismutase from Withania somnifera plant: gene cloning, expression and characterization of the recombinant protein

2011 ◽  
Vol 33 (10) ◽  
pp. 2057-2063 ◽  
Author(s):  
Raju Madanala ◽  
Vijayta Gupta ◽  
Farah Deeba ◽  
Santosh Kumar Upadhyay ◽  
Vivek Pandey ◽  
...  
Keyword(s):  
Superoxide Dismutase ◽  
Recombinant Protein ◽  
Gene Cloning ◽  
Withania Somnifera ◽  
Plant Gene
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