Molecular cloning and heterologous expression of acridone synthase from elicited Ruta graveolens L. cell suspension cultures

1995 ◽  
Vol 27 (4) ◽  
pp. 681-692 ◽  
Author(s):  
Kay Teja Junghanns ◽  
Richard Edward Kneusel ◽  
Alfred Baumert ◽  
Walter Maier ◽  
Detlef Gr�ger ◽  
...  
Keyword(s):  
Heterologous Expression ◽  
Cell Suspension ◽  
Molecular Cloning ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Ruta Graveolens ◽  
L Cell

scholarly journals Pisatin metabolism in pea ( Pisum sativum L.) cell suspension cultures

1997 ◽  
Vol 16 (5) ◽  
pp. 304-309
Author(s):  
Wlodzimierz Borejsza-Wysocki ◽  
Ewa Borejsza-Wysocka ◽  
Geza Hrazdina
Keyword(s):  
Pisum Sativum ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Pisum Sativum L ◽  
L Cell

Characterization of Volatile Constituents from Photomixotrophic Cell Suspension Cultures of Ruta graveolens

1984 ◽  
Vol 39 (6) ◽  
pp. 525-530 ◽  
Author(s):  
Friednch Drawert ◽  
Ralf G. Berger ◽  
Rolf Godelmann ◽  
Susanne Collin ◽  
Wolfgang Barz
Keyword(s):  
Acetic Acid ◽  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Callus Cultures ◽  
Suspension Cultures ◽  
Ruta Graveolens ◽  
Straight Chain ◽  
Methylbutyric Acid ◽  
Aliphatic Esters

Photomixotrophic cell suspension cultures of Ruta graveolens were qualitatively and quantita­tively analyzed by gaschromatography and mass spectroscopy for volatile compounds. The terpenoid hydrocarbons geijerene and pregeijerene, the C9-C13 methylketones and a series of aliphatic esters, respectively, were found as main constituents. The esters consisted of acetic acid, 2-methylbutyric acid and 3-methylbutyric acid which were esterified with straight chain or branched C8-C11 alcohols. The data are discussed in comparison to previous studies on callus cultures.

Elicitor-induced Changes of Enzyme Activities Related to Isoflavone and Pterocarpan Accumulation in Chickpea (Cicer arietinum L.) Cell Suspension Cultures

1988 ◽  
Vol 43 (7-8) ◽  
pp. 536-544 ◽  
Author(s):  
Susanne Daniel ◽  
Walter Hinderer ◽  
Wolfgang Barz
Keyword(s):  
Cell Culture ◽  
Cell Suspension ◽  
Cicer Arietinum ◽  
Enzyme Activities ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Biochanin A ◽  
Primary Metabolism ◽  
Cicer Arietinum L ◽  
L Cell

The extractable activities of thirteen enzymes of primary and secondary metabolism have been measured in chickpea (Cicer arietinum L.) cell suspension cultures after treatment with an elicitor from the fungus Ascochyta rabiei (Pass.) Lab. The cell culture, derived from the A. rabiei resistant cultivar ILC 3279, constitutively accumulated the isoflavones biochanin A and formononetin together with their 7-O-glucosides and the 7-O-glucoside-6″-malonates. After elicitor application the cells rapidly form the pterocarpan phytoalexins medicarpin and maackiain. Among the enzymes of primary metabolism only the glucose 6-phosphate dehydrogenase exhibited a significant increase in activity with a maximum four hours after application of the elicitor. In phenylpropane metabolism the activities of phenylalanine ammonia lyase and chalcone synthase were enhanced by the elicitor and exhibited highest levels after four hours. In contrast the chalcone isomerase activity was not influenced by the elicitor. A substantial enhancement occurred with the isoflavone 7-O-glucosyltransferase activity eight hours after elicitor application. The results suggest that in this cell culture the elicitor-induced biosynthesis of pterocarpan phytoalexins was accompanied with a rapid and transient increase of those enzyme activities which are located at branching points of related pathways, i.e. pentose phosphate cycle, general phenylpropane metabolism, flavonoid formation and isoflavone conjugation.

Glycyrrhiza glabra (Linn.) and Lavandula officinalis (L.) cell suspension cultures-based biotransformation of β-artemether

2011 ◽  
Vol 65 (3-4) ◽  
pp. 646-650 ◽  
Author(s):  
Suman Patel ◽  
Rashmi Gaur ◽  
Mohita Upadhyaya ◽  
Archana Mathur ◽  
Ajay K. Mathur ◽  
...  
Keyword(s):  
Cell Suspension ◽  
Cell Suspension Cultures ◽  
Suspension Cultures ◽  
Glycyrrhiza Glabra ◽  
L Cell ◽  
Lavandula Officinalis
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