Molecular cloning, in vitro expression and characterization of a plant squalene synthetase cDNA

1996 ◽  
Vol 30 (6) ◽  
pp. 1139-1151 ◽  
Author(s):  
Kathleen M. Hanley ◽  
Olivier Nicolas ◽  
Timothy B. Donaldson ◽  
Constance Smith-Monroy ◽  
Gordon W. Robinson ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
In Vitro Expression ◽  
Squalene Synthetase

Molecular cloning, in vitro expression and functional characterization of canine ADAMTS13

2012 ◽  
Vol 93 (1) ◽  
pp. 213-218 ◽  
Author(s):  
H. Maruyama ◽  
K. Ito ◽  
K. Okabayashi ◽  
M. Sakai ◽  
R. Kano ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Functional Characterization ◽  
In Vitro Expression

Molecular cloning, in vitro expression and bioactivity of dog A proliferation-inducing ligand (APRIL)

2009 ◽  
Vol 128 (4) ◽  
pp. 407-412 ◽  
Author(s):  
Shu-Le Wang ◽  
Yu-Feng Cai ◽  
Qing-Ping Lin ◽  
Xiao-Ling Sheng ◽  
Yan Shui ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
In Vitro Expression

scholarly journals Molecular cloning and characterization of the yeast gene for squalene synthetase.

1991 ◽  
Vol 88 (14) ◽  
pp. 6038-6042 ◽  
Author(s):  
S. M. Jennings ◽  
Y. H. Tsay ◽  
T. M. Fisch ◽  
G. W. Robinson
Keyword(s):  
Molecular Cloning ◽  
Yeast Gene ◽  
Squalene Synthetase

scholarly journals Molecular Cloning of ILP-2, a Novel Member of the Inhibitor of Apoptosis Protein Family

2001 ◽  
Vol 21 (13) ◽  
pp. 4292-4301 ◽  
Author(s):  
Bettina W. M. Richter ◽  
Samy S. Mir ◽  
Lisa J. Eiben ◽  
Jennifer Lewis ◽  
Stephanie Birkey Reffey ◽  
...  
Keyword(s):  
Molecular Cloning ◽  
Inhibitor Of Apoptosis ◽  
Physical Interaction ◽  
Caspase 9 ◽  
Inhibitor Of Apoptosis Protein ◽  
Normal Tissues ◽  
Apoptosis Protein ◽  
Processed Form

ABSTRACT Inhibitor of apoptosis protein (IAP)-like protein-1 (ILP-1) (also known as X-linked IAP [XIAP] and mammalian IAP homolog A [MIHA]) is a potent inhibitor of apoptosis and exerts its effects, at least in part, by the direct association with and inhibition of specific caspases. Here, we describe the molecular cloning and characterization of a human gene related to ILP-1, termed ILP-2. Despite high homology to ILP-1, ILP-2 is encoded by a distinct gene, which in normal tissues is expressed solely in testis. In contrast to ILP-1, overexpression of ILP-2 had no protective effect on apoptosis mediated by Fas (also known as CD95) or tumor necrosis factor. However, ILP-2 potently inhibited apoptosis induced by overexpression of Bax or by coexpression of caspase 9 with Apaf-1, and preincubation of cytosolic extracts with ILP-2 abrogated caspase activation in vitro. A processed form of caspase 9 could be coprecipitated with ILP-2 from cells, suggesting a physical interaction between ILP-2 and caspase 9. Thus, ILP-2 is a novel IAP family member with restricted specificity for caspase 9.

Cloning and In Vitro Expression and Characterization of the Androgen Receptor and Isolation of Estrogen Receptor α from the Fathead Minnow (Pimephales promelas)

2004 ◽  
Vol 38 (23) ◽  
pp. 6314-6321 ◽  
Author(s):  
Vickie S. Wilson ◽  
Mary C. Cardon ◽  
Joseph Thornton ◽  
Joseph J. Korte ◽  
Gerald T. Ankley ◽  
...  
Keyword(s):  
Estrogen Receptor ◽  
Androgen Receptor ◽  
Fathead Minnow ◽  
Pimephales Promelas ◽  
Estrogen Receptor Α ◽  
In Vitro Expression
Sign in / Sign up

Export Citation Format

Share Document