Purification and N-terminal sequencing of style glycoproteins associated with self-incompatibility in Petunia hybrida

1990 ◽  
Vol 14 (1) ◽  
pp. 93-102 ◽  
Author(s):  
Wim J. Broothaerts ◽  
André van Laere ◽  
Raf Witters ◽  
Gisèle Préaux ◽  
Benny Decock ◽  
...  
1975 ◽  
Vol 25 (2) ◽  
pp. 93-95 ◽  
Author(s):  
Hidejiro TAKAHASHI

1985 ◽  
Vol 16 (4) ◽  
pp. 233-245 ◽  
Author(s):  
K.R. Shivanna ◽  
Neelam Sharma

1991 ◽  
Vol 4 (4) ◽  
pp. 258-266 ◽  
Author(s):  
W. Broothaerts ◽  
P. Vanvinckenroye ◽  
B. Decock ◽  
J. Van Damme ◽  
J. C. Vendrig

1986 ◽  
Vol 82 (1) ◽  
pp. 1-10
Author(s):  
L. Carraro ◽  
G. Lombardo ◽  
F.M. Gerola

Self-, cross- and non-pollinated styles of Petunia hybrida were treated with 3–3′-diaminobenzidine for the ultrastructural localization of peroxidase activity. Wall peroxidases were absent in cross-pollinated styles, but they were detectable as an osmiophilic deposit on the cell walls of the outer portion of the transmitting tissue in self- and non-pollinated styles. The cell layer showing peroxidase activity was thicker in the self-pollinated styles than in the non-pollinated ones. In accordance with current hypotheses on the mechanism involved in pollen incompatibility process, it is suggested: that wall peroxidases present in the cells of the outer portion of the transmitting tissue are involved in the gametophytic self-incompatibility of Petunia; that self-pollination causes an increase in the number of cells involved in the rejection process; that non-pollinated styles, which are characterized by the presence of cell wall peroxidases in the outer portion of the transmitting tissue, are ‘prepared’ to a certain extent for the rejection of incompatible pollen tubes. The removal of peroxidase activity thus seems to be an important step in the compatible pollination process.


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