Synthetic Oligosaccharides Mimicking Fungal Cell Wall Polysaccharides

2019 ◽  
pp. 1-16 ◽  
Author(s):  
Vadim B. Krylov ◽  
Nikolay E. Nifantiev
Keyword(s):  
Cell Wall ◽  
Fungal Cell Wall ◽  
Cell Wall Polysaccharides ◽  
Fungal Cell ◽  
Synthetic Oligosaccharides

scholarly journals Potential of Chemically Synthesized Oligosaccharides To Define the Carbohydrate Moieties of the Fungal Cell Wall Responsible for the Human Immune Response, Using Aspergillus fumigatus Galactomannan as a Model

mSphere ◽  
2020 ◽  
Vol 5 (1) ◽  
Author(s):  
Sarah Sze Wah Wong ◽  
Vadim B. Krylov ◽  
Dmitry A. Argunov ◽  
Alexander A. Karelin ◽  
Jean-Phillipe Bouchara ◽  
...  
Keyword(s):  
Immune Response ◽  
Cell Wall ◽  
Aspergillus Fumigatus ◽  
Fungal Cell Wall ◽  
Human Pathogens ◽  
Cell Wall Polysaccharides ◽  
Fungal Cell ◽  
Content Type ◽  
Human Immune Response ◽  
Synthetic Oligosaccharides

ABSTRACT Methodologies to identify epitopes or ligands of the fungal cell wall polysaccharides influencing the immune response of human pathogens have to date been imperfect. Using the galactomannan (GM) of Aspergillus fumigatus as a model, we have shown that synthetic oligosaccharides of distinct structures representing key fragments of cell wall polysaccharides are the most precise tools to study the serological and immunomodulatory properties of a fungal polysaccharide.

scholarly journals M-ficolin is present in Aspergillus fumigatus infected lung and modulates epithelial cell immune responses elicited by fungal cell wall polysaccharides

Virulence ◽  
2017 ◽  
Vol 8 (8) ◽  
pp. 1870-1879 ◽  
Author(s):  
Kasper Jensen ◽  
Kit P. Lund ◽  
Kimmie B. Christensen ◽  
Anne T. Holm ◽  
Lalit Kumar Dubey ◽  
...  
Keyword(s):  
Cell Wall ◽  
Epithelial Cell ◽  
Immune Responses ◽  
Aspergillus Fumigatus ◽  
Fungal Cell Wall ◽  
Cell Wall Polysaccharides ◽  
Fungal Cell

Fungal cell wall polysaccharides isolated from Discula destructiva spp.

2007 ◽  
Vol 342 (8) ◽  
pp. 1138-1143 ◽  
Author(s):  
Oussama Ahrazem ◽  
Alicia Prieto ◽  
Juan Antonio Leal ◽  
M. Inmaculada Giménez-Abián ◽  
Jesús Jiménez-Barbero ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fungal Cell Wall ◽  
Cell Wall Polysaccharides ◽  
Fungal Cell ◽  
Discula Destructiva

scholarly journals The interaction with fungal cell wall polysaccharides determines the salt tolerance of antifungal plant defensins

2019 ◽  
Vol 5 ◽  
pp. 100026 ◽  
Author(s):  
Mark R. Bleackley ◽  
Charlotte S. Dawson ◽  
Jennifer A.E. Payne ◽  
Peta J. Harvey ◽  
K. Johan Rosengren ◽  
...  
Keyword(s):  
Cell Wall ◽  
Salt Tolerance ◽  
Fungal Cell Wall ◽  
Cell Wall Polysaccharides ◽  
Fungal Cell ◽  
Plant Defensins

Structural studies of fungal cell-wall polysaccharides from two strains of Talaromyces flavus

1994 ◽  
Vol 251 ◽  
pp. 315-325 ◽  
Author(s):  
Enrique Parra ◽  
Jesús Jiménez-Barbero ◽  
Manuel Bernabé ◽  
Juan Antonio Leal ◽  
Alicia Prieto ◽  
...  
Keyword(s):  
Cell Wall ◽  
Fungal Cell Wall ◽  
Structural Studies ◽  
Cell Wall Polysaccharides ◽  
Fungal Cell ◽  
Talaromyces Flavus

scholarly journals Analysis and application of a suite of recombinant endo-β(1,3)-d-glucanases for studying fungal cell walls

2021 ◽  
Vol 20 (1) ◽  
Author(s):  
Vanessa S. D. Carvalho ◽  
Laura Gómez-Delgado ◽  
M. Ángeles Curto ◽  
M. Belén Moreno ◽  
Pilar Pérez ◽  
...  
Keyword(s):  
Cell Wall ◽  
Environmental Changes ◽  
Antifungal Drugs ◽  
Fungal Cell Wall ◽  
Chemical Degradation ◽  
Cell Integrity ◽  
Cell Wall Degrading Enzymes ◽  
Cell Wall Polysaccharides ◽  
Fungal Cell ◽  
Commercial Applications

Abstract Background The fungal cell wall is an essential and robust external structure that protects the cell from the environment. It is mainly composed of polysaccharides with different functions, some of which are necessary for cell integrity. Thus, the process of fractionation and analysis of cell wall polysaccharides is useful for studying the function and relevance of each polysaccharide, as well as for developing a variety of practical and commercial applications. This method can be used to study the mechanisms that regulate cell morphogenesis and integrity, giving rise to information that could be applied in the design of new antifungal drugs. Nonetheless, for this method to be reliable, the availability of trustworthy commercial recombinant cell wall degrading enzymes with non-contaminating activities is vital. Results Here we examined the efficiency and reproducibility of 12 recombinant endo-β(1,3)-d-glucanases for specifically degrading the cell wall β(1,3)-d-glucan by using a fast and reliable protocol of fractionation and analysis of the fission yeast cell wall. This protocol combines enzymatic and chemical degradation to fractionate the cell wall into the four main polymers: galactomannoproteins, α-glucan, β(1,3)-d-glucan and β(1,6)-d-glucan. We found that the GH16 endo-β(1,3)-d-glucanase PfLam16A from Pyrococcus furiosus was able to completely and reproducibly degrade β(1,3)-d-glucan without causing the release of other polymers. The cell wall degradation caused by PfLam16A was similar to that of Quantazyme, a recombinant endo-β(1,3)-d-glucanase no longer commercially available. Moreover, other recombinant β(1,3)-d-glucanases caused either incomplete or excessive degradation, suggesting deficient access to the substrate or release of other polysaccharides. Conclusions The discovery of a reliable and efficient recombinant endo-β(1,3)-d-glucanase, capable of replacing the previously mentioned enzyme, will be useful for carrying out studies requiring the digestion of the fungal cell wall β(1,3)-d-glucan. This new commercial endo-β(1,3)-d-glucanase will allow the study of the cell wall composition under different conditions, along the cell cycle, in response to environmental changes or in cell wall mutants. Furthermore, this enzyme will also be greatly valuable for other practical and commercial applications such as genome research, chromosomes extraction, cell transformation, protoplast formation, cell fusion, cell disruption, industrial processes and studies of new antifungals that specifically target cell wall synthesis.

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