scholarly journals Acid-Induced Membrane Fusion by the Hemagglutinin Protein and Its Role in Influenza Virus Biology

2014 ◽  
pp. 93-116 ◽  
Author(s):  
Charles J. Russell
Keyword(s):  
Influenza Virus ◽  
Membrane Fusion ◽  
Induced Membrane ◽  
Hemagglutinin Protein ◽  
Virus Biology

Deacylation of influenza virus hemagglutinin does not affect the kinetics of low pH induced membrane fusion

1996 ◽  
Vol 431 (S6) ◽  
pp. R257-R258 ◽  
Author(s):  
Britta Schroth ◽  
Hans C. Philipp ◽  
Michael Veit ◽  
Michael F. G. Schmidt ◽  
Andreas Herrmann
Keyword(s):  
Influenza Virus ◽  
Membrane Fusion ◽  
Low Ph ◽  
Induced Membrane ◽  
Influenza Virus Hemagglutinin ◽  
Kinetics Of

scholarly journals Lipid interactions of the hemagglutinin HA2 NH2-terminal segment during influenza virus-induced membrane fusion.

1992 ◽  
Vol 267 (28) ◽  
pp. 20225-20232
Author(s):  
M Tsurudome ◽  
R Glück ◽  
R Graf ◽  
R Falchetto ◽  
U Schaller ◽  
...  
Keyword(s):  
Influenza Virus ◽  
Membrane Fusion ◽  
Terminal Segment ◽  
Induced Membrane ◽  
Lipid Interactions

scholarly journals Structural Features of Membrane Fusion between Influenza Virus and Liposome as Revealed by Quick-Freezing Electron Microscopy

1997 ◽  
Vol 137 (5) ◽  
pp. 1041-1056 ◽  
Author(s):  
Toku Kanaseki ◽  
Kazunori Kawasaki ◽  
Masayuki Murata ◽  
Yoko Ikeuchi ◽  
Shun-ichi Ohnishi
Keyword(s):  
Electron Microscopy ◽  
Influenza Virus ◽  
Membrane Fusion ◽  
Lipid Bilayer ◽  
Structural Features ◽  
Fusion Pore ◽  
Morphological Evidence ◽  
Quick Freezing ◽  
Hemagglutinin Protein ◽  
Liposomal Membranes

The structure of membrane fusion intermediates between the A/PR/8(H1N1) strain of influenza virus and a liposome composed of egg phosphatidylcholine, cholesterol, and glycophorin was studied using quick-freezing electron microscopy. Fusion by viral hemagglutinin protein was induced at pH 5.0 and 23°C. After a 19-s incubation under these conditions, small protrusions with a diameter of 10–20 nm were found on the fractured convex faces of the liposomal membranes, and small pits complementary to the protrusions were found on the concave faces. The protrusions and pits corresponded to fractured parts of outward bendings of the lipid bilayer or “microprotrusions of the lipid bilayer.” At the loci of the protrusions and pits, liposomal membranes had local contacts with viral membranes. In many cases both the protrusions and the pits were aligned in regular polygonal arrangements, which were thought to reflect the array of hemagglutinin spikes on the viral surface. These structures were induced only when the medium was acidic with the virus present. Based on these observations, it was concluded that the microprotrusions of the lipid bilayer are induced by hemagglutinin protein. Furthermore, morphological evidence for the formation of the “initial fusion pore” at the microprotrusion was obtained. The protrusion on the convex face sometimes had a tiny hole with a diameter of <4 nm in the center. The pits transformed into narrow membrane connections <10 nm in width, bridging viruses and liposomes. The structures of the fusion pore and fusion neck with larger sizes were also observed, indicating growth of the protrusions and pits to distinct fusion sites. We propose that the microprotrusion of the lipid bilayer is a fusion intermediate induced by hemagglutinin protein, and suggest that the extraordinarily high curvature of this membrane structure is a clue to the onset of fusion. The possible architecture of the fusion intermediate is discussed with regard to the localization of intramembrane particles at the microprotrusion.

Calorimetric detection of influenza virus induced membrane fusion

Biochemistry ◽  
1995 ◽  
Vol 34 (17) ◽  
pp. 5705-5711 ◽  
Author(s):  
Simon Nebel ◽  
Ingrid Bartoldus ◽  
Toon Stegmann
Keyword(s):  
Influenza Virus ◽  
Membrane Fusion ◽  
Induced Membrane

scholarly journals Influence of Salt Bridges in the Avian Influenza Virus HA on Acid-Induced Membrane Fusion and Pathogenicity

2012 ◽  
Vol 102 (3) ◽  
pp. 604a
Author(s):  
Caroline Mair ◽  
P. Sivaramakrishna Rachakonda ◽  
Thomas Korte ◽  
Andreas Herrmann
Keyword(s):  
Influenza Virus ◽  
Avian Influenza ◽  
Avian Influenza Virus ◽  
Membrane Fusion ◽  
Salt Bridges ◽  
Induced Membrane

An ultrastructural approach to influenza virus hemagglutinin-induced membrane fusion

1989 ◽  
Vol 31 (4) ◽  
pp. 460
Author(s):  
KoertN.J. Burger ◽  
Gerhard Knoll ◽  
Gerrit van Meer ◽  
ArieJ. Verkleij
Keyword(s):  
Influenza Virus ◽  
Membrane Fusion ◽  
Induced Membrane ◽  
Influenza Virus Hemagglutinin

scholarly journals Intermediates in influenza virus PR/8 haemagglutinin-induced membrane fusion

1994 ◽  
Vol 75 (2) ◽  
pp. 395-399 ◽  
Author(s):  
C. C. Pak ◽  
M. Krumbiegel ◽  
R. Blumenthal
Keyword(s):  
Influenza Virus ◽  
Membrane Fusion ◽  
Induced Membrane
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