Methods for Analyzing Sphingosine-1-Phosphate Signaling in Human and Mouse Primary Mast Cells

2017 ◽  
pp. 21-30
Author(s):  
Alena P. Chumanevich ◽  
Piper A. Wedman ◽  
Carole A. Oskeritzian
Keyword(s):  
Mast Cells ◽  
Sphingosine 1 Phosphate ◽  
Human And Mouse

scholarly journals Sphingosine 1-Phosphate-Mediated Trafficking of Pathogenic Th2 and Mast Cells for the Control of Food Allergy

2007 ◽  
Vol 179 (3) ◽  
pp. 1577-1585 ◽  
Author(s):  
Yosuke Kurashima ◽  
Jun Kunisawa ◽  
Morio Higuchi ◽  
Masashi Gohda ◽  
Izumi Ishikawa ◽  
...  
Keyword(s):  
Food Allergy ◽  
Mast Cells ◽  
Sphingosine 1 Phosphate

scholarly journals Lipocalin 2: A New Antimicrobial in Mast Cells

2019 ◽  
Vol 20 (10) ◽  
pp. 2380 ◽  
Author(s):  
Yu-Ling Chang ◽  
Zhenping Wang ◽  
Satomi Igawa ◽  
Jae Eun Choi ◽  
Tyler Werbel ◽  
...  
Keyword(s):  
Mast Cells ◽  
Antimicrobial Peptides ◽  
Antimicrobial Peptide ◽  
Bacterial Growth ◽  
Immune Defense ◽  
Lipocalin 2 ◽  
Vitro Assay ◽  
E Coli ◽  
Sphingosine 1 Phosphate

Mast cells (MCs) play a significant role in the innate immune defense against bacterial infection through the release of cytokines and antimicrobial peptides. However, their antimicrobial function is still only partially described. We therefore hypothesized that MCs express additional antimicrobial peptides. In this study, we used FANTOM 5 transcriptome data to identify for the first time that MCs express lipocalin 2 (LCN2), a known inhibitor of bacterial growth. Using MCs derived from mice which were deficient in LCN2, we showed that this antimicrobial peptide is an important component of the MCs’ antimicrobial activity against Escherichia coli (E. coli). Since sphingosine-1-phosphate receptors (S1PRs) on MCs are known to regulate their function during infections, we hypothesized that S1P could activate LCN2 production in MCs. Using an in vitro assay, we demonstrated that S1P enhances MCs antimicrobial peptide production and increases the capacity of MCs to directly kill S. aureus and E. coli via an LCN2 release. In conclusion, we showed that LCN2 is expressed by MCs and plays a role in their capacity to inhibit bacterial growth.

scholarly journals Early Activation of Sphingosine Kinase in Mast Cells and Recruitment to FcεRI Are Mediated by Its Interaction with Lyn Kinase

2004 ◽  
Vol 24 (19) ◽  
pp. 8765-8777 ◽  
Author(s):  
Nicole Urtz ◽  
Ana Olivera ◽  
Elisa Bofill-Cardona ◽  
Robert Csonga ◽  
Andreas Billich ◽  
...  
Keyword(s):  
Mast Cells ◽  
Tyrosine Kinase ◽  
Kinase Activity ◽  
Immunoglobulin E ◽  
Sphingosine Kinase ◽  
Lipid Kinase ◽  
Lyn Kinase ◽  
Sphingosine 1 Phosphate ◽  
High Affinity Receptor

ABSTRACT Sphingosine kinase has been recognized as an essential signaling molecule that mediates the intracellular conversion of sphingosine to sphingosine-1-phosphate. In mast cells, induction of sphingosine kinase and generation of sphingosine-1-phosphate have been linked to the initial rise in Ca2+, released from internal stores, and to degranulation. These events either precede or are concomitant with the activation of phospholipase C-γ and the generation of inositol trisphosphate. Here we show that sphingosine kinase type 1 (SPHK1) interacts directly with the tyrosine kinase Lyn and that this interaction leads to the recruitment of this lipid kinase to the high-affinity receptor for immunoglobulin E (FcεRI). The interaction of SPHK1 with Lyn caused enhanced lipid and tyrosine kinase activity. After FcεRI triggering, enhanced sphingosine kinase activity was associated with FcεRI in sphingolipid-enriched rafts of mast cells. Bone marrow-derived mast cells from Lyn−/ − mice, compared to syngeneic wild-type cells, were defective in the initial induction of SPHK1 activity, and the defect was overcome by retroviral Lyn expression. These findings position the activation of SPHK1 as an FcεRI proximal event.

scholarly journals IL-33 Induces a Hyporesponsive Phenotype in Human and Mouse Mast Cells

2012 ◽  
Vol 190 (2) ◽  
pp. 531-538 ◽  
Author(s):  
Mi-Yeon Jung ◽  
Daniel Smrž ◽  
Avanti Desai ◽  
Geethani Bandara ◽  
Tomonobu Ito ◽  
...  
Keyword(s):  
Mast Cells ◽  
Human And Mouse

scholarly journals 352 Neuromedin U directly activates human and mouse skin mast cells

2017 ◽  
Vol 137 (10) ◽  
pp. S253
Author(s):  
Y. Matsuo ◽  
Y. Yanase ◽  
S. Tanaka ◽  
K. Furuta ◽  
M. Hide
Keyword(s):  
Mast Cells ◽  
Mouse Skin ◽  
Human And Mouse

scholarly journals Differential effects of C5a on human and mouse mast cells may be mediated by C5aR and C5L2

2010 ◽  
Vol 6 (S1) ◽  
Author(s):  
P Pundir ◽  
CH Sheen ◽  
A Catalli ◽  
V Karpov ◽  
K Cianflone ◽  
...  
Keyword(s):  
Mast Cells ◽  
Differential Effects ◽  
Human And Mouse

Mast cells and sphingosine-1-phosphate underlie prelesional remodeling in a mouse model of eczema

Allergy ◽  
2017 ◽  
Vol 73 (2) ◽  
pp. 405-415 ◽  
Author(s):  
P. A. Wedman ◽  
A. Aladhami ◽  
A. P. Chumanevich ◽  
J. W. Fuseler ◽  
C. A. Oskeritzian
Keyword(s):  
Mast Cells ◽  
Mouse Model ◽  
Sphingosine 1 Phosphate

scholarly journals The Balance between Sphingosine and Sphingosine-1-Phosphate Is Decisive for Mast Cell Activation after Fc∈ Receptor I Triggering

1999 ◽  
Vol 190 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Eva E. Prieschl ◽  
Robert Csonga ◽  
Veronica Novotny ◽  
Gary E. Kikuchi ◽  
Thomas Baumruker
Keyword(s):  
Mast Cells ◽  
Protein Kinase ◽  
Cytokine Production ◽  
Fc Receptor ◽  
Mitogen Activated Protein Kinase ◽  
Mast Cell Activation ◽  
Signaling Cascades ◽  
Mitogen Activated Protein ◽  
Sphingosine 1 Phosphate ◽  
Kinase Pathway

Over the last few years, sphingolipids have been identified as potent second messenger molecules modulating cell growth and activation. A newly emerging facet to this class of lipids suggests a picture where the balance between two counterregulatory lipids (as shown in the particular example of ceramide and sphingosine-1-phosphate in T lymphocyte apoptosis) determines the cell fate by setting the stage for various protein signaling cascades. Here, we provide a further example of such a decisive balance composed of the two lipids sphingosine and sphingosine-1-phosphate that determines the allergic responsiveness of mast cells. High intracellular concentrations of sphingosine act as a potent inhibitor of the immunoglobulin (Ig)E plus antigen–mediated leukotriene synthesis and cytokine production by preventing activation of the mitogen-activated protein kinase pathway. In contrast, high intracellular levels of sphingosine-1-phosphate, also secreted by allergically stimulated mast cells, activate the mitogen-activated protein kinase pathway, resulting in hexosaminidase and leukotriene release, or in combination with ionomycin, give cytokine production. Equivalent high concentrations of sphingosine-1-phosphate are dominant over sphingosine as they counteract its inhibitory potential. Therefore, it might be inferred that sphingosine-kinase is pivotal to the activation of signaling cascades initiated at the Fc∈ receptor I by modulating the balance of the counterregulatory lipids.

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