Batch Transduction of Transposon Mutant Libraries for Rapid Phenotype Screening in Staphylococcus

2015 ◽  
pp. 75-81
Author(s):  
Katherine L. Maliszewski
Keyword(s):  
Phenotype Screening

Different subjective and objective measures and cut-points of physical activity in frailty phenotype screening: A need for standardization?

2021 ◽  
pp. 104479
Author(s):  
Duarte Barros ◽  
Flávia Borges-Machado ◽  
Wagner Andrade da Silva ◽  
Alinne Nascimento ◽  
Joana Carvalho ◽  
...  
Keyword(s):  
Physical Activity ◽  
Objective Measures ◽  
Frailty Phenotype ◽  
Cut Points ◽  
Phenotype Screening

scholarly journals Combined genomic and phenotype screening reveals secretory factor SPINK1 as an invasion and survival factor associated with patient prognosis in breast cancer

2011 ◽  
Vol 3 (8) ◽  
pp. 451-464 ◽  
Author(s):  
Wendy WeiJia Soon ◽  
Lance David Miller ◽  
Michael A. Black ◽  
Cyril Dalmasso ◽  
Xiu Bin Chan ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Survival Factor ◽  
Phenotype Screening ◽  
Patient Prognosis

scholarly journals Application of Whole Genome Sequencing to Understand Diversity and Presence of Genes Associated with Sanitizer Tolerance in Listeria monocytogenes from Produce Handling Sources

Foods ◽  
2021 ◽  
Vol 10 (10) ◽  
pp. 2454
Author(s):  
Rebecca N. Bland ◽  
Jared D. Johnson ◽  
Joy G. Waite-Cusic ◽  
Alexandra J. Weisberg ◽  
Elizabeth R. Riutta ◽  
...  
Keyword(s):  
Whole Genome Sequencing ◽  
Genome Sequencing ◽  
Whole Genome ◽  
Nucleotide Polymorphisms ◽  
Single Nucleotide ◽  
Phenotype Screening ◽  
Screening Assays ◽  
Contamination Events ◽  
Sequence Types ◽  
Potential Use

Recent listeriosis outbreaks linked to fresh produce suggest the need to better understand and mitigate L. monocytogenes contamination in packing and processing environments. Using whole genome sequencing (WGS) and phenotype screening assays for sanitizer tolerance, we characterized 48 L. monocytogenes isolates previously recovered from environmental samples in five produce handling facilities. Within the studied population there were 10 sequence types (STs) and 16 cgMLST types (CTs). Pairwise single nucleotide polymorphisms (SNPs) ranged from 0 to 3047 SNPs within a CT, revealing closely and distantly related isolates indicative of both sporadic and continuous contamination events within the facility. Within Facility 1, we identified a closely related cluster (0–2 SNPs) of isolates belonging to clonal complex 37 (CC37; CT9492), with isolates recovered during sampling events 1-year apart and in various locations inside and outside the facility. The accessory genome of these CC37 isolates varied from 94 to 210 genes. Notable genetic elements and mutations amongst the isolates included the bcrABC cassette (2/48), associated with QAC tolerance; mutations in the actA gene on the Listeria pathogenicity island (LIPI) 1 (20/48); presence of LIPI-3 (21/48) and LIPI-4 (23/48). This work highlights the potential use of WGS in tracing the pathogen within a facility and understanding properties of L. monocytogenes in produce settings.

scholarly journals An In Vitro Partial Lesion Model of Differentiated Human Mesencephalic Neurons: Effect of Pericyte Secretome on Phenotypic Markers

2020 ◽  
Vol 70 (11) ◽  
pp. 1914-1925
Author(s):  
Abderahim Gaceb ◽  
Marco Barbariga ◽  
Gesine Paul
Keyword(s):  
Cell Death ◽  
Growth Factor ◽  
Substantia Nigra Pars Compacta ◽  
Progressive Degeneration ◽  
Brain Pericytes ◽  
Phenotype Screening ◽  
Partial Lesion ◽  
Marker Expression ◽  
6 Hydroxydopamine

Abstract Parkinson’s disease (PD) is characterised by the progressive degeneration of dopaminergic (DA) neurons in the substantia nigra pars compacta. Post-mortem data suggests that the loss of DA markers may long precede the cell death, leaving a window to rescue the DA phenotype. Screening for potential neuroprotective or restorative therapies, however, requires that partial lesions of DA neurons can be modelled in vitro. In order to establish a partial lesion model of DA neurons in vitro, we evaluated the effects of different exposure times to 1-methyl-4-phenylpyridinium (MPP+) and 6-hydroxydopamine (6-OHDA) on the cell survival and DA marker expression using DA neurons derived from the Lund human mesencephalic (LUHMES) cell line. We show that 24-h incubation with 50 μM of MPP+ or 6-h incubation with 100 μM of 6-OHDA leads to a significant decrease in the protein expression of DA markers without affecting overall cell death, consistent with a mild DA lesion. Using conditioned medium of human brain–derived pericytes stimulated with platelet-derived growth factor BB (PDGF-BB), we demonstrate a significant upregulation of DA markers. In conclusion, we provide an experimental model of an in vitro DA neuron partial lesion suitable to study different molecules and their potential neuroprotective or neurorestorative effects on the DA phenotype. We provide evidence that the secretome of brain pericytes stimulated via PDGF-BB/PDGFRβ affects DA marker expression and may represent one possible mechanism contributing to the neurorestoration previously observed in PD by this growth factor.

scholarly journals High-throughput mutation, selection, and phenotype screening of mutant methanogenic archaea

2016 ◽  
Vol 131 ◽  
pp. 113-121 ◽  
Author(s):  
Mary E. Walter ◽  
Alicia Ortiz ◽  
Casey Sondgeroth ◽  
Nathan M. Sindt ◽  
Nikolas Duszenko ◽  
...  
Keyword(s):  
High Throughput ◽  
Methanogenic Archaea ◽  
Phenotype Screening

scholarly journals A Transgenic Mouse Model for High Content, Cell Cycle Phenotype Screening in Live Primary Cells

Cell Cycle ◽  
2007 ◽  
Vol 6 (18) ◽  
pp. 2276-2283 ◽  
Author(s):  
Richard O. Burney ◽  
Alan I. Lee ◽  
Denise E. Leong ◽  
Joshua T. Jones ◽  
Angela T. Hahn ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Transgenic Mouse Model ◽  
Primary Cells ◽  
Phenotype Screening

scholarly journals Data and animal management software for large-scale phenotype screening

2006 ◽  
Vol 17 (4) ◽  
pp. 288-297 ◽  
Author(s):  
Keith A. Ching ◽  
Michael P. Cooke ◽  
Lisa M. Tarantino ◽  
Hilmar Lapp
Keyword(s):  
Large Scale ◽  
Management Software ◽  
Animal Management ◽  
Phenotype Screening

Challenges and Solutions for Frailty: Role of Nurses

2019 ◽  
Vol 15 (2) ◽  
pp. 90-94
Author(s):  
Mohanasudnari SK ◽  
Padmaja A
Keyword(s):  
Screening Tool ◽  
Clinical Stage ◽  
Adverse Outcomes ◽  
Frailty Syndrome ◽  
Term Care ◽  
Phenotype Screening ◽  
Impending Death ◽  
Physiologic Function

Abstract: Frailty refers to a loss of physiologic function that makes a person susceptible to disability from minor stresses. The frailty syndrome is a collection of symptoms primarily due to the aging-related loss and dysfunction of skeletal muscle and bone, that place older adults at increased levels of risk for disability, dependency, falls, injury, ospitalization, need for long term care, and mortality. Frailty is thought distinguishable from disability and comorbidity. Frailty always implies multisystem dysfunction. Severity of frailty range subclinical to a clinical stage to impending death. It is Important to recognize and treat the frailty syndrome before occurrence of any adverse outcomes. Fried's Phenotype screening tool identifies a person as being frail

Histopathology is required to identify and characterize myopathies in high-throughput phenotype screening of genetically engineered mice

2021 ◽  
pp. 030098582110305
Author(s):  
Peter Vogel ◽  
Robert W. Read ◽  
Gwenn M. Hansen ◽  
David R. Powell
Keyword(s):  
Mouse Models ◽  
High Throughput ◽  
Human Disease ◽  
High Throughput Screening ◽  
Screen Test ◽  
Screening Tests ◽  
Preclinical Research ◽  
Screening Programs ◽  
Genetically Engineered Mice ◽  
Phenotype Screening

The development of mouse models that replicate the genetic and pathological features of human disease is important in preclinical research because these types of models enable the completion of meaningful pharmacokinetic, safety, and efficacy studies. Numerous relevant mouse models of human disease have been discovered in high-throughput screening programs, but there are important specific phenotypes revealed by histopathology that are not reliably detected by any other physiological or behavioral screening tests. As part of comprehensive phenotypic analyses of over 4000 knockout (KO) mice, histopathology identified 12 lines of KO mice with lesions indicative of an autosomal recessive myopathy. This report includes a brief summary of histological and other findings in these 12 lines. Notably, the inverted screen test detected muscle weakness in only 4 of these 12 lines ( Scyl1, Plpp7, Chkb, and Asnsd1), all 4 of which have been previously recognized and published. In contrast, 6 of 8 KO lines showing negative or inconclusive findings on the inverted screen test ( Plppr2, Pnpla7, Tenm1, Srpk3, Sidt2, Yif1b, Mrs2, and Pnpla2) had not been previously identified as having myopathies. These findings support the need to include histopathology in phenotype screening protocols in order to identify novel genetic myopathies that are not clinically evident or not detected by the inverted screen test.

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