scholarly journals A Transgenic Mouse Model for High Content, Cell Cycle Phenotype Screening in Live Primary Cells

Cell Cycle ◽  
2007 ◽  
Vol 6 (18) ◽  
pp. 2276-2283 ◽  
Author(s):  
Richard O. Burney ◽  
Alan I. Lee ◽  
Denise E. Leong ◽  
Joshua T. Jones ◽  
Angela T. Hahn ◽  
...  
Keyword(s):  
Cell Cycle ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Transgenic Mouse Model ◽  
Primary Cells ◽  
Phenotype Screening

scholarly journals The CRE luc Bioluminescence Transgenic Mouse Model for Detecting Ligand Activation of GPCRs

2013 ◽  
Vol 19 (2) ◽  
pp. 232-241 ◽  
Author(s):  
Holly Dressler ◽  
Kyriakos Economides ◽  
Sarah Favara ◽  
Nancy N. Wu ◽  
Zhen Pang ◽  
...  
Keyword(s):  
Mouse Model ◽  
Transgenic Mouse ◽  
Expression Profiles ◽  
Tissue Expression ◽  
Transgenic Mouse Model ◽  
Luciferase Reporter ◽  
Primary Cells ◽  
Compound Libraries ◽  
Cellular Environment

Numerous assays have been developed to investigate the interactions between G-protein–coupled receptors (GPCRs) and their ligands since GPCRs are key therapeutic targets. Reporter-based assays using the cAMP response element (CRE) coupled with bioluminescence from a luciferase reporter have been used extensively in vitro with high-throughput screens (HTS) of large chemical compound libraries. We have generated a transgenic mouse model (CRE luc) with a luciferase reporter under the control of a synthetic promoter that contains several CREs, which supports real-time bioimaging of GPCR ligand activity in whole animals, tissues, or primary cells. In the CRE luc model, GPCR signaling through the cAMP pathway can be detected from the target GPCR that is in a native cellular environment with a full complement of associated receptors and membrane constituents. Multiple independent lines have been produced by random integration of the transgene, resulting in tissue expression profiles covering the major organs. The goal of the CRE luc model is to accelerate the transition from HTS to profiling of GPCR small-molecule leads in preclinical animal disease models, as well as define the mechanism of action of GPCR drugs in three experimental formats: primary cells, tissue homogenates, and whole animals.

scholarly journals A New Transgenic Mouse Model for the Study of Cell Cycle Control in Megakaryocytes

Stem Cells ◽  
1996 ◽  
Vol 14 (S1) ◽  
pp. 181-187 ◽  
Author(s):  
Alexander Thompson ◽  
Zhuhui Zhao ◽  
Daniel Ladd ◽  
Jeffrey Zimmet ◽  
Katya Ravid
Keyword(s):  
Cell Cycle ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Cell Cycle Control ◽  
Transgenic Mouse Model

scholarly journals Cell-Cycle Markers in a Transgenic Mouse Model of Human Tauopathy

2006 ◽  
Vol 168 (3) ◽  
pp. 878-887 ◽  
Author(s):  
Patrice Delobel ◽  
Isabelle Lavenir ◽  
Bernardino Ghetti ◽  
Max Holzer ◽  
Michel Goedert
Keyword(s):  
Cell Cycle ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Transgenic Mouse Model

scholarly journals Decoding c-Myc networks of cell cycle and apoptosis regulated genes in a transgenic mouse model of papillary lung adenocarcinomas

Oncotarget ◽  
2015 ◽  
Vol 6 (31) ◽  
pp. 31569-31592 ◽  
Author(s):  
Yari Ciribilli ◽  
Prashant Singh ◽  
Reinhard Spanel ◽  
Alberto Inga ◽  
Jürgen Borlak
Keyword(s):  
Cell Cycle ◽  
Mouse Model ◽  
Transgenic Mouse ◽  
Transgenic Mouse Model ◽  
Lung Adenocarcinomas
Sign in / Sign up

Export Citation Format

Share Document