Mortality Due to Nosocomial Infection with Klebsiella pneumoniae ESBL+

2017 ◽  
pp. 19-26 ◽  
Author(s):  
Katarzyna Starzyk-Łuszcz ◽  
Tadeusz M. Zielonka ◽  
Joanna Jakubik ◽  
Katarzyna Życińska
Keyword(s):  
Klebsiella Pneumoniae ◽  
Nosocomial Infection

scholarly journals The issue of nosocomial infection caused by Klebsiella pneumoniae blaNDM-type

2020 ◽  
Vol 15 (2) ◽  
pp. 124-133
Author(s):  
Olga Mironenko ◽  
◽  
Victoria Selnitseva ◽  
Lidia Soprun ◽  
Elena Shmushkevich ◽  
...  
Keyword(s):  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Molecular Genetic ◽  
Mechanisms Of Resistance ◽  
Infectious Process ◽  
Saint Petersburg ◽  
Microbiological Study ◽  
Wide Range ◽  
Almost All ◽  
Emergence Of Resistance

The article presents information about circulating isolates Klebsiella pneumoniae in a hospital megapolis with properties of hypervirulence and simultaneous multiresistance. The resulting K. pneumonia isolates are of particular importance due to the emergence of resistance to almost all β-lactams due to the presence of carbapenemase metal-β-lactamase. Furthermore, the isolated strains producing carbapenemases possess mechanisms of resistance to a wide range of antimicrobial preparations, and the types of infectious process caused by carbapenemazo-producing enterobacteries are characterized by a high lethality level. Microbiological, biochemical, biophysical, molecular-genetic, biological, bioinformational and statistical methods of research were used in the work. A prospective method was used to identify the source of the infections. In the first stage, a microbiological study was carried out on biomaterials obtained from patients treated in a hospital in Saint Petersburg. After a microbiological study, 52 isolates of K. pneumoniae were obtained, 53.8 % of isolates had a hypermucoid phenotype and 98 % had carbapenemases:blaNDM type — 49 (92 %), blaNDM+OXA-48-like — 3 (8 %). Isolates with two new phenotypes have been isolated (no. 2511 and no. 2512). Isolates of no. 2512 LD50 had 10*2 BAC/ml, and plasmids such as Incfib(Mar), Inchi1b, and Incr were also found, with Incr-A plasmid emitted encoding resistance to fluoroquinolone: aac(6’)-Ib-cr and to β-lactam antibiotics: blaTEM-1B. The described data confirm the opinions of the researchers about the possible formation of a new “super pathogen” — instantaneously hypervirulent and plural resistant strain of K. pneumoniae.

scholarly journals Complete Genome Sequences of Lytic Polysaccharide-Degrading Klebsiella pneumoniae Bacteriophages vB_KpnS_FZ10, vB_KpnP_FZ12, vB_KpnM_FZ14, and vB_KpnS_FZ41

2019 ◽  
Vol 8 (39) ◽  
Author(s):  
Fedor Zurabov ◽  
Evgeniy Zhilenkov
Keyword(s):  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Nosocomial Infections ◽  
Complete Genome ◽  
Microbial Pathogens ◽  
Genome Sequences

Members of the genus Klebsiella are among the leading microbial pathogens associated with nosocomial infection. At the same time, most nosocomial infections are caused by strains resistant to antibiotics. Here, we announce the complete genome sequences of four lytic polysaccharide-degrading bacteriophages, which will be used in complex therapeutic preparations.

scholarly journals Identification and Minisequencing-Based Discrimination of SHV β-Lactamases in Nosocomial Infection-Associated Klebsiella pneumoniae in Brisbane, Australia

2002 ◽  
Vol 46 (3) ◽  
pp. 659-664 ◽  
Author(s):  
Christopher Howard ◽  
Angela van Daal ◽  
Gregory Kelly ◽  
Jacqueline Schooneveldt ◽  
Graeme Nimmo ◽  
...  
Keyword(s):  
Antibiotic Resistance ◽  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Nucleotide Change ◽  
Princess Alexandra Hospital ◽  
Single Nucleotide ◽  
Extended Spectrum ◽  
Copy Numbers ◽  
Encoding Gene ◽  
Encoding Genes

ABSTRACT Extended-spectrum β-lactamases (ESBLs) are active against oxyimino cephalosporins and monobactams. Twenty-one Klebsiella pneumoniae isolates obtained between 1991 and 1995 at the Princess Alexandra Hospital in Brisbane, Australia, were subject to amplification and sequencing of the SHV β-lactamase-encoding genes. Thirteen strains were phenotypically ESBL positive. Of these, six strains carried the bla SHV-2a gene and seven strains carried the bla SHV-12 gene. Eight strains were phenotypically ESBL negative. Of these, seven strains carried the non-ESBL bla SHV-11 gene and one strain carried the non-ESBL bla SHV-1 gene. There was complete correspondence between the ESBL phenotype and the presence or absence of an ESBL-encoding gene(s). In addition, it was determined that of the 13 ESBL-positive strains, at least 4 carried copies of a non-ESBL-encoding gene in addition to the bla SHV-2a or bla SHV12 gene. A minisequencing-based assay was developed to discriminate the different SHV classes. This technique, termed “first-nucleotide change,” involves the identification of the base added to a primer in a single-nucleotide extension reaction. The assay targeted polymorphisms at the first bases of codons 238 and 240 and reliably discriminated ESBL-positive strains from ESBL-negative strains and also distinguished strains carrying bla SHV-2a from strains carrying bla SHV-12. In addition, this method was used to demonstrate an association between the relative copy numbers of bla SHV genes in individual strains and the levels of antibiotic resistance.

PCR characterization and typing of Klebsiella pneumoniae using capsular type-specific, variable number tandem repeat and virulence gene targets

2010 ◽  
Vol 59 (5) ◽  
pp. 541-547 ◽  
Author(s):  
Jane F. Turton ◽  
Claire Perry ◽  
Suzanne Elgohari ◽  
Catherine V. Hampton
Keyword(s):  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Tandem Repeat ◽  
Internal Transcribed Spacer ◽  
Variable Number Tandem Repeat ◽  
Virulence Gene ◽  
Invasive Disease ◽  
Variable Number ◽  
Capsular Type ◽  
Liver Abscesses

A multiplex PCR is described which detects capsular types K1, K2, K5, K54 and K57, which are those most associated with invasive disease or pathogenicity, a further capsular type (K20), two putative virulence factors (rmpA and wcaG) and the 16S–23S internal transcribed spacer unit of Klebsiella pneumoniae, facilitating identification of this organism. wcaG encodes capsular fucose production and was associated with capsular types K1 and K54, but was also found in strains of other capsular types; 18 of the 543 isolates screened were PCR-positive for this gene. An eight-locus variable number tandem repeat (VNTR) scheme was designed, which provided discrimination at a level similar to that afforded by PFGE among a panel of 36 isolates representing 29 PFGE types. All isolates tested of the virulent K1 clone of CC23, associated with pyogenic liver abscesses, shared the same VNTR profile, which may be helpful in identifying this clone; such isolates were also PCR-positive for allS. These methods provide a rapid means of characterizing and typing isolates of this important agent of community-acquired and nosocomial infection.

scholarly journals POLA BAKTERI AEROB PENYEBAB INFEKSI NOSOKOMIAL PADA RUANGAN NEONATAL INTENSIVE CARE UNIT (NICU) BLU RSUP PROF. DR. R. D KANDOU MANADO

2015 ◽  
Vol 3 (1) ◽  
Author(s):  
Malindra Saleh ◽  
Fredine E. S . Rares ◽  
Standy Soeliongan
Keyword(s):  
Intensive Care Unit ◽  
Bacillus Subtilis ◽  
Intensive Care ◽  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Neonatal Intensive Care Unit ◽  
Neonatal Intensive Care ◽  
Enterobacter Agglomerans ◽  
Proteus Vulgaris ◽  
Serratia Liquefaciens

Abstract: Nosocomial infection is an infection which occurs in the hospital caused by microbacteria from the hospital. Infection in neonatal is a serious issue in each hospital until today. Neonatal Intensive Care Unit (NICU) is a care unit for newborn who needs special care. Nosocomial infection is often happening to babies in NICU. The objective is to determine the pattern of aerob bacteria causes nosocomial infection in Neonatal Intensive Care Unit (NICU) BLU RSUP Prof. Dr. R. D. Kandou Manado. The research used descriptive method with prospective study. The amount of samples are 30 and was taken according to category of care room, room instruments, medical equipments and air. Based on the results, there are 11 species of bacterias included Bacillus subtilis 13 samples (43,3%), Serratia liquefaciens 4 samples (13,3%), Lactobacillus 3 samples (10%), Enterobacter agglomerans 2 samples (6,7%) and Klebsiella pneumoniae 2 samples (6,7%), Proteus mirabilis 1 sample (3,3%), Proteus vulgaris 1 sample (3,3%), Streptococcus non hemoliticus 1 sample (3,3%), Diplococcus 1 sample (3,3%), gram-positive Coccus 1 sample (3,3%) and gram-negative Coccus 1 sample (3,3%).Keywords: nosocomial infection, NICU, aerob bacteriaAbstrak: Infeksi nosokomial adalah infeksi yang terjadi di rumah sakit oleh kuman yang berasal dari rumah sakit. Infeksi nosokomial yang terjadi pada bayi baru lahir sampai saat ini merupakan masalah yang serius disetiap rumah sakit. Neonatal Intensive Care Unit (NICU) merupakan unit perawatan untuk bayi baru lahir yang memerlukan perawatan khusus. Infeksi nosokomial sering terjadi terutama pada bayi yang mendapat perawatan di NICU. Tujuan dari penelitian ini yaitu untuk mengetahui pola bakteri aerob penyebab infeksi nosokomial pada ruangan Neonatal Intensive Care Unit (NICU) BLU RSUP Prof. Dr. R. D Kandou Manado. Penelitian ini menggunakan metode deskriptif dengan pendekatan studi prospektif. Sampel yang diteliti berjumlah 30 sampel dan di ambil berdasarkan kategori ruang perawatan, perabotan ruangan, peralatan medis dan udara. Berdasarkan hasil penelitian ditemukan 11 spesies bakteri yaitu Bacillus subtilis 13 sampel (43,3%), Serratia liquefaciens 4 sampel (13,3%), Lactobacillus 3 sampel (10%), Enterobacter agglomerans 2 sampel (6,7%) dan Klebsiella pneumoniae 2 sampel (6,7%), Proteus mirabilis 1 sampel (3,3%), Proteus vulgaris 1 sampel (3,3%), Streptococcus non hemolitikus 1 sampel (3,3%), Diplokokus 1 sampel (3,3%), Kokus gram positif 1 sampel (3,3%) dan Kokus gram negatif 1 sampel (3,3%).Kata kunci: infeksi nosokomial, NICU, bakteri aerob

scholarly journals Pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado

2016 ◽  
Vol 4 (2) ◽  
Author(s):  
Rizka Matoka ◽  
Olivia Waworuntu ◽  
Fredine Rares
Keyword(s):  
Health Care ◽  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Emergency Room ◽  
Enterobacter Aerogenes ◽  
Aerobic Bacteria ◽  
Bacillus Sp ◽  
Cross Sectional ◽  
Serratia Liquefaciens ◽  
Health Care Associated Infection

Abstract: Nosocomial infection or Health-care Associated Infection (HAIs) according to the WHO in 2011 are infection to patient acquired during a hospital, after ≥48 hours and ≤30 days after out of the hospital. Factor of infection can be differentiated between patients, nurses, doctors, hospital employed, hospital environment and patient visitor or family. The purpose to know the pattern of aerobic bacteria that could potentially to involve nosocomial infection in the Emergency room of Obstetric and Gynecology (IRDO) Prof. Dr. R. D. Kandou Manado. Type a descriptive observational research with cross sectional study approach. The sampling on the walls, floors, medical equipment, room furniture and the air in the IRDO Prof. Dr. R. D. Kandou Hospital Manado. The results of the 27 samples found bacteria Bacillus sp.(46,55%), Staphylococcus sp. (18,97%), Streptococcus sp. (10,34%), Klebsiella pneumoniae (8,62%), Enterobacter aerogenes, Serratia liquefaciens, Enterobacter cloacea, proteus sp. (3,45%), Neisseria sp. (1,72%).Conclusion: The most aerobic bacteria in the Emergency room of Obstetric and Gynecology (IRDO) Prof. Dr. R. D. Kandou Manado is Bacillus sp.(46,55%).Keywords: Emergency Obstetric and Gynecology, nosocomial infection, aerobic bacteria. Abstrak: Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) adalah suatu instalasi penanggulangan awal penderita rawat darurat untuk mencegah kematian, dalam menangani kesehatan reproduksi wanita saat tidak hamil ataupun pada saat hamil, persalinan, dan nifas. Infeksi nosokomial atau Health-care Associated Infection (HAIs) menurut WHO 2011 merupakan infeksi yang didapat pasien selama di rumah sakit, setelah ≥ 48 jam dan ≤ 30 hari setelah keluar dari rumah sakit. Faktor terjadinya infeksi bisa dibebakan antar pasien, perawat, dokter, karyawan rumah sakit, lingkungan rumah sakit, dan pengunjung atau keluarga pasien. Tujuan untuk mengetahui pola bakteri aerob yang berpotensi menyebabkan infeksi nosokomial pada ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado. Jenis penelitian deskriptif observational dengan pendekatan studi cross sectional. Pengambilan sampel pada dinding, lantai, peralatan medis, perabotan ruangan, udara di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado. Hasil penelitian dari 27 sampel ditemukan bakteri Bacillus sp.(46,55%), Staphylococcus sp. (18,97%), Streptococcus sp. (10,34%), Klebsiella pneumoniae (8,62%), Enterobacter aerogenes, Serratia liquefaciens, Enterobacter cloacea, proteus sp. (3,45%), Neisseria sp. (1,72%). Simpulan: Bakteri aerob di ruangan Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO) RSUP Prof. Dr. R. D. Kandou Manado yang terbanyak ialah Bacillus sp.(46,55%).Kata kunci: Instalasi Rawat Darurat Obstetri dan Ginekologi (IRDO), Infeksi nosokomial, bakteri aerob

Detection and characterisation of extended spectrum β-lactamases in Klebsiella pneumoniae causing nosocomial infection

Pathology ◽  
1998 ◽  
Vol 30 (2) ◽  
pp. 164-168 ◽  
Author(s):  
Jacqueline M. Schooneveldt ◽  
Graeme R. Nilvimo ◽  
Phil Giffard
Keyword(s):  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Extended Spectrum

scholarly journals Profiles of microorganisms isolated from neonates’ blood cultures, incubators, cradles, ventilators, washbasins, and health-workers of Libreville University Hospital Neonatal Service: focus on infection prevention and control measures

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Eliane Kuissi Kamgaing ◽  
Jean-Charles Ndong ◽  
Léonard Kouegnigan Rerambiah ◽  
Joel Fleury Djoba Siawaya
Keyword(s):  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Health Workers ◽  
Medical Personnel ◽  
Control Measures ◽  
Sub Saharan Africa ◽  
University Hospital ◽  
Infection Prevention And Control ◽  
Sub Saharan ◽  
Prevention And Control Measures

Background: Nosocomial infection outbreaks in neonatal services are a serious healthcare concern in both developed and developing countries, but few studies have been conducted in sub-Saharan Africa. Objective: This study explored the etiology of septicemia in neonates and associated patterns of antimicrobial susceptibility in Gabon. Methods: We analyzed cultures from neonates’ blood and swabs from medical personnel and equipment located in the neonatology service. Results: Sixty-eight microorganisms were isolated from the medical personnel and equipment; 46 microorganisms were isolated from neonates’ blood culture. Klebsiella pneumoniae spp pneumoniae was the most common bacteria found in both (30.6% and 26.9%, respectively). All Klebsiella pneumoniae spp pneumonia isolates were resistant to amoxicillin with clavulanic acid, gentamycin resistance ranged from 93% to 100%, and cephalosporin resistance ranged from 33.3% to 47%. Conclusions: Awareness of the etiology, prevalence, and outcome of nosocomial infection is the first and most important step to appropriate interventions

scholarly journals POLA KETAHANAN (RESISTEN) DAN KEPEKAAN (SENSITIVITAS) KUMAN TERHADAP ANTIMIKROBA

2018 ◽  
Vol 16 (2) ◽  
pp. 58
Author(s):  
Y F Tallulembang ◽  
Nurhayana Sennang ◽  
Benny Rusli
Keyword(s):  
Developing Countries ◽  
Klebsiella Pneumoniae ◽  
Nosocomial Infection ◽  
Antimicrobial Therapy ◽  
Staphylococcus Epidermidis ◽  
Descriptive Study ◽  
Klebsiella Pneumonia ◽  
Enterobacter Agglomerans ◽  
Antimicrobial Drugs ◽  
Collective Data

In the developing countries including Indonesia, there still have problems with infectious diseases. Generally the hospitalized patientsin the Surgery section through have antimicrobial therapy. The treatment is intended to prevent nosocomial infection that usually foundin overnight treatment patients at the department of surgery. This study is carried out to know the pattern of micro-organism’s resistanceand sensitivity against various antimicrobial at department of surgery, Dr. Wahidin Sudirohusodo Hospital Makassar, January–June2008. A descriptive study of retrospectively collective data was carried out on 160 specimens by sensitivity test at the department ofsurgery, Dr. Wahidin Sudirohusodo Makassar Hospital, started from January up to June 2008. The five common bacterial found in thisstudy where Staphylococcus epidermidis, Klebsiella pneumonia, Enterobacter agglomerans, Staphylococcus saprophytic and Escherichiacoli. The sensitive antimicrobial drugs found where Vancomicin, Sulbactam/Cepoperazone, Cefepim, Gentamicin, Ceftazidim, Novobiocin,followed by the resistance antimicrobial were Methicillin, Tetracycline, Cefuroxim, Erythromycin, and Doxyciclin. The most bacterial typefound was Klebsiella pneumoniae, the most sensitive antimicrobial was Vancomicin and the most resistances were Methicillin.

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