Synergistic Activity for Natural and Synthetic Inhibitors of Angiogenesis Induced by Murine Sarcoma L-1 and Human Kidney Cancer Cells

2017 ◽  
pp. 91-104 ◽  
Author(s):  
Barbara J. Bałan ◽  
Andrzej K. Siwicki ◽  
Krzysztof Pastewka ◽  
Urszula Demkow ◽  
Piotr Skopiński ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Kidney Cancer ◽  
Human Kidney ◽  
Synergistic Activity ◽  
Murine Sarcoma ◽  
Natural And Synthetic

scholarly journals The enhanced 32 P labeling of CDP-diacylglycerol in c-myc gene expressed human kidney cancer cells

FEBS Letters ◽  
1987 ◽  
Vol 212 (1) ◽  
pp. 159-162 ◽  
Author(s):  
Yoshinobu Kubota ◽  
Taro Shuin ◽  
Masahiro Yao ◽  
Hiroko Inoue ◽  
Tohru Yoshioka
Keyword(s):  
Cancer Cells ◽  
Kidney Cancer ◽  
Human Kidney ◽  
Myc Gene

scholarly journals Оценка эффективности противоопухолевого воздействия и индукции апоптоза в клетках карциномы почки человека биологически активными добавками, содержащими ресвератрол, индол-3-карбинол и кордицепин, флуоресцентными методами визуализации

2021 ◽  
Vol 129 (6) ◽  
pp. 727
Author(s):  
Н.В. Полуконова ◽  
Д.С. Исаев ◽  
А.М. Мыльников ◽  
А.Б. Бучарская ◽  
А.В. Полуконова ◽  
...  
Keyword(s):  
Cancer Cells ◽  
Kidney Cancer ◽  
Human Tumor ◽  
Human Kidney ◽  
Antitumor Agent ◽  
Cytostatic Activity ◽  
Antitumor Effects ◽  
Human Renal Cell Carcinoma ◽  
Low Concentrations

We compared the effectiveness of antitumor effects and apoptosis induction on human renal cell carcinoma A498 extracts of cruciferous plant material (indole-3-carbinol), Chinese mushroom (cordycepin), French red wine (resveratrol) at low concentrations after 24 and 48 h using fluorescent methods of imaging apoptosis and necrosis in human tumor cells in vitro . Propidium iodide and acridine orange were used as dyes in the "alive and dead" test, which allowed us to detect the number of dead cells and cells in which apoptosis had started. It was found that indole-3-carbinol at low concentrations (0.0288 and 0.1152 mg/ml) has a pronounced cytotoxic and cytostatic activity against human kidney cancer cells, significantly exceeding the action of resveratrol at the same concentrations. At the same time, cordycepin has no cytotoxic and cytostatic activity at these concentrations. Indole-3-carbinol also has the most pronounced apoptotic activity at concentrations of 0.0144-0.1152 mg/ml, after 48 h the number of kidney cancer cells in apoptosis increases by 6.8-10 times compared to control. It is concluded that indole-3-carbinol is a promising antitumor agent for use in the complex therapy of patients with kidney cancer.

scholarly journals An IDH-independent mechanism of DNA hypermethylation upon VHL inactivation in cancer

2020 ◽  
Author(s):  
Artem V. Artemov ◽  
Svetlana Zhenilo ◽  
Daria Kaplun ◽  
Alexey Starshin ◽  
Alexey Sokolov ◽  
...  
Keyword(s):  
Dna Methylation ◽  
Cancer Cells ◽  
Kidney Cancer ◽  
Cancer Progression ◽  
Human Kidney ◽  
Dna Demethylation ◽  
List Type ◽  
Wild Type ◽  
Dna Hypermethylation ◽  
Endogenous Expression

Hypermethylation of tumor suppressors and other aberrations of DNA methylation in tumors play a significant role in cancer progression. DNA methylation can be affected by various environmental conditions including hypoxia. The response to hypoxia is mainly achieved through activation of the transcription program associated with HIF1a transcription factor. VHL inactivation by genetic or epigenetic events, which also induces aberrant activation of HIF1a, is the most common driver event for renal cancer. With whole-genome bisulfite sequencing and LC-MS, we demonstrated that VHL inactivation induced global genome hypermethylation in human kidney cancer cells under normoxic conditions. This effect was reverted by exogenous expression of wild-type VHL. We show that global genome hypermethylation in VHL mutants can be explained by transcriptional changes in MDH and L2HGDH genes that cause the accumulation of 2-hydroxyglutarate—a metabolite that inhibits DNA demethylation by Tet enzymes. Unlike the known cases of DNA hypermethylation in cancer, 2-hydroxyglutarate was accumulated in IDH wild type cells.Key pointsInactivation of VHL gene leads to genome hypermethylation in kidney cancer cells. The DNA methylation phenotype can be rescued by endogenous expression of wild-type VHL.DNA hypermethylation can be attributed to the accumulation of a Tet inhibitor 2-hydroxyglutarateThe accumulation of 2-hydroxyglutarate in IDH wild-type cells is explained by gene expression changes in key metabolic enzymes (malate dehydrogenase MDH and 2-hydroxyglutrarate dehydrogenase L2HGDH).

scholarly journals A PRELIMINARY STUDY TO DETERMINE THE ACTIVITY OF TOPOISOMERASE II IN HUMAN KIDNEY CANCER CELLS WITH DNA UNKNOTTING METHOD

1992 ◽  
Vol 83 (9) ◽  
pp. 1511-1516
Author(s):  
Katsuyuki Sano ◽  
Taro Shuuin ◽  
Masahiko Hosaka ◽  
Tadashi Ikegami ◽  
Yukio Shiomi
Keyword(s):  
Cancer Cells ◽  
Kidney Cancer ◽  
Topoisomerase Ii ◽  
Human Kidney ◽  
Preliminary Study

scholarly journals A Vitamin D Receptor-Alkylating Derivative of 1α,25-Dihydroxyvitamin D3 Inhibits Growth of Human Kidney Cancer Cells and Suppresses Tumor Growth

2010 ◽  
Vol 3 (12) ◽  
pp. 1596-1607 ◽  
Author(s):  
James R. Lambert ◽  
Vikram J. Eddy ◽  
Christian D. Young ◽  
Kelly S. Persons ◽  
Sibaji Sarkar ◽  
...  
Keyword(s):  
Vitamin D ◽  
Tumor Growth ◽  
Cancer Cells ◽  
Kidney Cancer ◽  
Vitamin D Receptor ◽  
Human Kidney ◽  
Dihydroxyvitamin D3

scholarly journals Synergistic Action of Stilbenes in Muscadine Grape Berry Extract Shows Better Cytotoxic Potential Against Cancer Cells Than Resveratrol Alone

Biomedicines ◽  
2019 ◽  
Vol 7 (4) ◽  
pp. 96 ◽  
Author(s):  
Subramani Paranthaman Balasubramani ◽  
Mohammad Atikur Rahman ◽  
Sheikh Mehboob Basha
Keyword(s):  
Cytotoxic Activity ◽  
Cancer Cells ◽  
Biological Activities ◽  
Grape Berry ◽  
Similar Response ◽  
Synergistic Activity ◽  
Anti Cancer ◽  
Muscadine Grape ◽  
Berry Extracts ◽  
Cancer Activity

Muscadine grape is rich in stilbenes, which include resveratrol, piceid, viniferin, pterostilbene, etc. Resveratrol has been extensively studied for its biological activities; however, the synergistic effect of stilbene compounds in berry extracts is poorly understood. The aim of this study was to evaluate the anti-cancer activity of stilbene-rich muscadine berry extract and pure resveratrol. Stilbenes were extracted from ripened berries of muscadine grape cultivars, Pineapple, and Southern Home. HPLC analysis was performed to determine quantity of stilbenes. The extracts were tested for their cytotoxic activity against A549 (lung carcinoma cells), triple negative breast cancer (HCC-1806) and HepG2 (human liver cancer) cells. The stilbene-rich extracts of the muscadine berry extracts showed cytotoxic activity against all of the cells tested. The extracts at 1 μg/mL induced death in 50–80% of cells by 72 h of treatment. About 50 μg/mL of resveratrol was required to induce a similar response in the cells. Further, modulation of genes involved in tumor progression and suppression was significantly (p < 0.0005) higher with the HepG2 cells treated with stilbene-rich berry extracts than the pure resveratrol. This shows that the synergistic activity of stilbenes present in muscadine grape berries have more potent anti-cancer activity than the resveratrol alone.

scholarly journals Regulation of thrombospondin-1 by natural and synthetic progestins in human breast cancer cells

2009 ◽  
Vol 16 (3) ◽  
pp. 809-817 ◽  
Author(s):  
Salman M Hyder ◽  
Yayun Liang ◽  
Jianbo Wu ◽  
Vanessa Welbern
Keyword(s):  
Breast Cancer ◽  
Cancer Cells ◽  
Breast Cancer Cells ◽  
P53 Protein ◽  
Endothelial Cell Proliferation ◽  
Ru 486 ◽  
Thrombospondin 1 ◽  
Treated Breast ◽  
Endothelial Growth Factor ◽  
Natural And Synthetic

Our recent studies show that progestins induce vascular endothelial growth factor (VEGF) in breast cancer cells that express mutant p53 protein. Here, we show that natural and synthetic progestins also induce thrombospondin-1 (TSP-1) mRNA and protein in T47-D and BT-474 breast cancer cells. Antiprogestin RU-486 inhibits the induction of VEGF and TSP-1 by progestins, suggesting that this effect of progestin is mediated by the progesterone receptor (PR). Actinomycin-D, but not puromycin, also blocks progestin-dependent induction of TSP-1. A putative progestin-response element was identified in the human TSP-1 promoter, which is consistent with the hypothesis that a progestin–PR complex might directly regulate transcription of the TSP-1 gene in human cells. Conditioned medium from progestin-treated breast cancer cells stimulates endothelial cell proliferation in the absence though not in the presence of antibody to TSP-1, indicating that TSP-1 secreted by breast cancer cells could be pro-angiogenic. Since tumor cell-derived TSP-1 has the potential to promote angiogenesis in the tumor microenvironment, it could be a potential target for breast cancer therapy.

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