Effect of Natural and Synthetic Estrogens on A549 Lung Cancer Cells:  Correlation of Chemical Structures with Cytotoxic Effects

2005 ◽  
Vol 18 (3) ◽  
pp. 466-474 ◽  
Author(s):  
Silvana Andreescu ◽  
Omowunmi A. Sadik ◽  
Dennis W. McGee
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
Cytotoxic Effects ◽  
Chemical Structures ◽  
Synthetic Estrogens ◽  
A549 Lung ◽  
Natural And Synthetic

scholarly journals Poly(1-vinylimidazole) polyplexes as novel therapeutic gene carriers for lung cancer therapy

2020 ◽  
Vol 11 ◽  
pp. 354-369
Author(s):  
Gayathri Kandasamy ◽  
Elena N Danilovtseva ◽  
Vadim V Annenkov ◽  
Uma Maheswari Krishnan
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
Vascular Endothelial ◽  
Cytotoxic Effects ◽  
Lung Cancer Therapy ◽  
Interfering Rna ◽  
Endothelial Growth Factor ◽  
A549 Lung

The present work explores the ability of poly(1-vinylimidazole) (PVI) to complex small interfering RNA (siRNA) silencing vascular endothelial growth factor (VEGF) and the in vitro efficiency of the formed complexes in A549 lung cancer cells. The polyplex formed was found to exhibit 66% complexation efficiency. The complexation was confirmed by gel retardation assays, FTIR and thermal analysis. The blank PVI polymer was not toxic to cells. The polyplex was found to exhibit excellent internalization and escaped the endosome effectively. The polyplex was more effective than free siRNA in silencing VEGF in lung cancer cells. The silencing of VEGF was quantified using Western blot and was also reflected in the depletion of HIF-1α levels in the cells treated with the polyplex. VEGF silencing by the polyplex was found to augment the cytotoxic effects of the chemotherapeutic agent 5-fluorouracil. Microarray analysis of the mRNA isolated from cells treated with free siRNA and the polyplex reveal that the VEGF silencing by the polyplex also altered the expression levels of several other genes that have been connected to the proliferation and invasion of lung cancer cells. These results indicate that the PVI complexes can be an effective agent to counter lung cancer.

scholarly journals Poly (1-Vinyl Imidazole) Polyplexes as Novel Therapeutic Gene Carriers for Lung Cancer Therapy

2019 ◽  
Author(s):  
Gayathri kandasamy ◽  
Elena N Danilovtseva ◽  
Vadim annenkov ◽  
Uma maheswari Krishnan
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
Vascular Endothelial ◽  
Cytotoxic Effects ◽  
Lung Cancer Therapy ◽  
Si Rna ◽  
Endothelial Growth Factor ◽  
A549 Lung

The present work explores the ability of poly (1-vinyl imidazole) to complex si-RNA against vascular endothelial growth factor (VEGF) and its in vitro efficiency in A549 lung cancer cells. The polyplex formed was found to exhibit 66% complexation efficiency. The complexation was confirmed by gel retardation assay, FTIR and thermal analysis. The blank PVI polymer was not toxic to cells. The polyplex was found to exhibit excellent internalization and escaped the endosome effectively. The polyplex was more effective than the free si-RNA in silencing VEGF in lung cancer cells. The silencing of VEGF was quantified using Western blot which was also reflected in depletion of HIF-1a levels in the cells treated with the polyplex. VEGF silencing by the polyplex was found to augment the cytotoxic effects of the chemotherapeutic agent 5-fluorouracil. Microarray analysis of the mRNA isolated from cells treated with free siRNA and polyplex reveal that the superior VEGF silencing by the polyplex altered the expression levels of several other genes that have been implicated in the proliferation and invasion of lung cancer cells. These results indicate that PVI complexed anti-VEGF can be an effective strategy to counter lung cancer.

Targeted delivery of doxorubicin to A549 lung cancer cells by CXCR4 antagonist conjugated PLGA nanoparticles

2014 ◽  
Vol 88 (2) ◽  
pp. 529-538 ◽  
Author(s):  
Chuda Chittasupho ◽  
Kriengsak Lirdprapamongkol ◽  
Prartana Kewsuwan ◽  
Narong Sarisuta
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Targeted Delivery ◽  
Plga Nanoparticles ◽  
Lung Cancer Cells ◽  
Cxcr4 Antagonist ◽  
A549 Lung

Moringa oleifera: Natural leaf extract with potential anti-cancerous effect on A549 lung cancer cells

Lung Cancer ◽  
2012 ◽  
Vol 77 ◽  
pp. S22 ◽  
Author(s):  
M. Dany ◽  
N. Madi ◽  
N. Nemer ◽  
M. Beyrouthy ◽  
S. Abdoun ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Leaf Extract ◽  
Moringa Oleifera ◽  
Lung Cancer Cells ◽  
Natural Leaf ◽  
A549 Lung

Quercetin metabolites inhibit MMP-2 expression in A549 lung cancer cells by PPAR-γ associated mechanisms

2016 ◽  
Vol 33 ◽  
pp. 45-53 ◽  
Author(s):  
Cheng-Hung Chuang ◽  
Chiao-Lin Yeh ◽  
Shu-Lan Yeh ◽  
En-Shyh Lin ◽  
Li-Yu Wang ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Lung Cancer Cells ◽  
Ppar Γ ◽  
A549 Lung

scholarly journals Gold-containing compound BDG-I inhibits the growth of A549 lung cancer cells through the deregulation of miRNA expression

2018 ◽  
Vol 26 (7) ◽  
pp. 1035-1043 ◽  
Author(s):  
Ali Alhoshani ◽  
A. Alrashdi ◽  
Khaled Alhosaini ◽  
Fawaz E. Alanazi ◽  
Nehad M. Alajez ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Mirna Expression ◽  
Lung Cancer Cells ◽  
A549 Lung
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