Detection of DNA Damage Induced by Hydroquinone and Catechol Using an Electrochemical DNA Biosensor

2009 ◽  
Vol 62 (9) ◽  
pp. 1181 ◽  
Author(s):  
Chuanhu Wang ◽  
Jun Zhao ◽  
Dapeng Zhang ◽  
Zhousheng Yang
Keyword(s):  
Dna Damage ◽  
Electrochemical Method ◽  
Differential Pulse ◽  
Acetate Buffer Solution ◽  
Dna Biosensors ◽  
Buffer Solution ◽  
Calf Thymus ◽  
Time Differential ◽  
Constant Potential ◽  
Anodic Peak Current

DNA damage induced by hydroquinone and catechol was detected by an electrochemical method. Calf thymus DNA was immobilized onto the surface of a pretreated glassy carbon electrode (GCE(ox)) to form a DNA/GCE(ox) modified electrode. Then the DNA/GCE(ox) was incubated in acetate buffer solution containing hydroquinone or catechol at a constant potential for the desired time. Differential pulse voltammetric experiments were then performed. The anodic peaks corresponding to the oxidation of guanisine and adenosine on the electrode could be observed on voltammetric curves. The experimental results showed that DNA damage could be detected using electrochemical DNA biosensors. The extent of DNA damage could be electrochemically recognized via the change of the anodic peak current. DNA damage induced by hydroquinone was greater than that by catechol. The response conditions were optimized with respect to DNA concentration, pH, ionic strength, and other variables.

scholarly journals Determination of Mercury (II) Ion on Aryl Amide-Type Podand-Modified Glassy Carbon Electrode

2011 ◽  
Vol 2011 ◽  
pp. 1-6 ◽  
Author(s):  
Sevgi Güney ◽  
Gülcemal Yıldız ◽  
Gönül Yapar
Keyword(s):  
Glassy Carbon Electrode ◽  
Modified Electrode ◽  
Glassy Carbon ◽  
Reference Electrode ◽  
Differential Pulse ◽  
Acetate Buffer Solution ◽  
Carbon Electrode ◽  
Modified Glassy Carbon Electrode ◽  
Buffer Solution

A new voltammetric sensor based on an aryl amide type podand, 1,8-bis(o-amidophenoxy)-3,6-dioxaoctane, (AAP) modified glassy carbon electrode, was described for the determination of trace level of mercury (II) ion by cyclic voltammetry (CV) and differential pulse voltammetry (DPV). A well-defined anodic peak corresponding to the oxidation of mercury on proposed electrode was obtained at 0.2 V versus Ag/AgCl reference electrode. The effect of experimental parameters on differential voltammetric peak currents was investigated in acetate buffer solution of pH 7.0 containing 1 × 10−1 mol L−1NaCl. Mercury (II) ion was preconcentrated at the modified electrode by forming complex with AAP under proper conditions and then reduced on the surface of the electrode. Interferences of Cu2+, Pb2+, Fe3+, Cd2+, and Zn2+ions were also studied at two different concentration ratios with respect to mercury (II) ions. The modified electrode was applied to the determination of mercury (II) ions in seawater sample.

scholarly journals Electrochemically Activated Screen-Printed Carbon Electrode for Determination of Ibuprofen

2021 ◽  
Vol 11 (21) ◽  
pp. 9908
Author(s):  
Katarzyna Tyszczuk-Rotko ◽  
Jędrzej Kozak ◽  
Anna Węzińska
Keyword(s):  
Supporting Electrolyte ◽  
Pharmaceutical Formulations ◽  
Differential Pulse ◽  
Acetate Buffer Solution ◽  
Carbon Electrode ◽  
Buffer Solution ◽  
Screen Printed Carbon Electrode ◽  
Relative Errors ◽  
Screen Printed

In this study, we present a simple, sensitive and selective analytical procedure for the ibuprofen (IBP) analysis using the commercially available screen-printed carbon electrode electrochemically activated (aSPCE) by cyclic voltammetry in 0.1 M NaOH. The quantitative determinations of IBP were carried out in 0.25 M acetate buffer solution of pH 4.5 ± 0.1 using the differential-pulse voltammetry (DPV). Different experimental parameters for DPV analysis were optimized, including pH and concentration of supporting electrolyte, amplitude (ΔEA), scan rate (ν) and modulation time (tm). The linear ranges of calibration curve were from 0.50–20.0 and 20.0–500.0 µM. The detection and quantification limits were estimated to be 0.059 and 0.20 µM. The aSPCE displayed satisfactory repeatability, reproducibility, and selectivity. Furthermore, the DPV procedure with the use of aSPCE was used to determination of IBP in pharmaceutical formulations. The results achieved by DPV show satisfactory agreement with those obtained by manufacturers (the relative errors are in the range of 3.1–4.7%).

Study of Analytical Parameters of a Differential Pulse Voltammetric Method using Chemically Modified Electrodes for Lead Analysis in Water

2018 ◽  
Vol 69 (9) ◽  
pp. 2311-2314 ◽  
Author(s):  
Ioana Georgiana Lazar ◽  
Elena Diacu ◽  
Gabriela Geanina Vasile ◽  
Eleonora Mihaela Ungureanu ◽  
Alexandru Anton Ivanov
Keyword(s):  
Water Samples ◽  
Modified Electrodes ◽  
Differential Pulse ◽  
Uncertainty Budget ◽  
Acetate Buffer Solution ◽  
Buffer Solution ◽  
Intermediate Precision ◽  
Voltammetric Method ◽  
Glassy Carbon Electrodes ◽  
Set Up

The development of a new voltammetric method for the determination of Pb2+ ions in water samples using glassy carbon electrodes modified with 2,6-bis((E)-2-(thiophen-2-yl)vinyl)-4-(4,6,8-trimethylazulen-1-yl)pyrylium perchlorate is here presented. The analytical parameters for differential pulse voltammetry (DPV) were established and the method was applied for Pb2+ ions determination in acetate buffer solution at pH 5.5. For the developed DPV method the linearity of calibration curve was set up between 20.71mg L-1 - 103.6 mg L-1 with a good correlation coefficient R2=0.9988, the intermediate precision was calculated for three lead concentrations:20, 60,100mg L-1.The obtained detection and quantification limits were 3.1 mg L-1, respectively 10.4 mg L-1. An uncertainty budget was developed and the value of expanded uncertainty was assessed. The optimized method was used to analyze Pb2+ ions from of water samples and good correlation with standard method was obtained.

Molecular Transformations in DNA under the Influence of UV-radiation

2020 ◽  
Vol 04 ◽  
Author(s):  
Vigen G. Barkhudaryan ◽  
Gayane V. Ananyan ◽  
Nelli H. Karapetyan
Keyword(s):  
Uv Radiation ◽  
Uv Irradiation ◽  
Absorption Spectroscopy ◽  
Calf Thymus Dna ◽  
Dilute Solutions ◽  
Concentrated Solutions ◽  
Buffer Solution ◽  
Low Concentration ◽  
Calf Thymus ◽  
Dna Solutions

Background: The processes of destruction and crosslinking of macromolecules occur simultaneously under the influence of ultraviolet (UV) radiation in synthetic polymers, dry DNA and their concentrated solutions. Objective: The effect of UV radiation on calf thymus DNA in dilute solutions subjected to UV- irradiation was studied in this work. Method: The calf thymus DNA was studied in dilute solutions using viscometry, absorption spectroscopy and electrophoresis. Results: It was shown, that at a low concentration of DNA in the buffer solution ([DNA] = 85 μg / ml) under the influence of UV radiation, the processes of destruction of macromolecules and an increase in their flexibility predominate, which is accompanied by a gradual decrease in the viscosity of their solution. In addition, due to the low concentration of the solution, intramolecular crosslinking of macromolecules predominates, which also reduces their size and, consequently, the viscosity of the solution. Conclusion: It was concluded, that in dilute DNA solutions, due to the predominance of the processes of intramolecular crosslinking of macromolecules over intermolecular, only constant processes of decreasing the sizes of DNA macromolecules occur. As a result, its solubility remains virtually unchanged during UV irradiation. The described comments are also excellently confirmed by the results of absorption spectroscopy and electrophoresis

scholarly journals Simultaneous electrochemical sensing of dihydroxy benzene isomers at cost-effective allura red polymeric film modified glassy carbon electrode

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Pattan-Siddappa Ganesh ◽  
Ganesh Shimoga ◽  
Seok-Han Lee ◽  
Sang-Youn Kim ◽  
Eno E. Ebenso
Keyword(s):  
Glassy Carbon Electrode ◽  
Glassy Carbon ◽  
Limit Of Detection ◽  
Tap Water ◽  
Differential Pulse ◽  
Oxidation Potential ◽  
Electrochemical Sensing ◽  
Carbon Electrode ◽  
Buffer Solution ◽  
Allura Red

Abstract Background A simple and simultaneous electrochemical sensing platform was fabricated by electropolymerization of allura red on glassy carbon electrode (GCE) for the interference-free detection of dihydroxy benzene isomers. Methods The modified working electrode was characterized by electrochemical and field emission scanning electron microscopy methods. The modified electrode showed excellent electrocatalytic activity for the electrooxidation of catechol (CC) and hydroquinone (HQ) at physiological pH of 7.4 by cyclic voltammetric (CV) and differential pulse voltammetric (DPV) techniques. Results The effective split in the overlapped oxidation signal of CC and HQ was achieved in a binary mixture with peak to peak separation of 0.102 V and 0.103 V by CV and DPV techniques. The electrode kinetics was found to be adsorption-controlled. The oxidation potential directly depends on the pH of the buffer solution, and it witnessed the transfer of equal number of protons and electrons in the redox phenomenon. Conclusions The limit of detection (LOD) for CC and HQ was calculated to be 0.126 μM and 0.132 μM in the linear range of 0 to 80.0 μM and 0 to 110.0 μM, respectively, by ultra-sensitive DPV technique. The practical applicability of the proposed sensor was evaluated for tap water sample analysis, and good recovery rates were observed. Graphical abstract Electrocatalytic interaction of ALR/GCE with dihydroxy benzene isomers.

TBAI Catalyzed Electrochemical C-H Bond Activation of 2-arylated-N-methoxyamides for the Synthesis of Phenanthridinones

Synlett ◽  
2021 ◽  
Author(s):  
Kripa Subramanian ◽  
Subhash L. Yedage ◽  
Kashish Sethi ◽  
Bhalchandra M. Bhanage
Keyword(s):  
Electrochemical Method ◽  
Bond Activation ◽  
Supporting Electrolyte ◽  
Bioactive Compound ◽  
Reaction Conditions ◽  
Redox Catalyst ◽  
Synthetic Utility ◽  
One Step ◽  
Constant Potential

An electrochemical method for the synthesis of phenanthridinones via constant potential electrolysis (CPE) mediated by <i>n</i>-Bu<sub>4</sub>NI (TBAI) has been reported. The protocol is metal and oxidant free and proceeds with 100% current efficiency. Here TBAI plays the dual role of the redox catalyst as well as supporting electrolyte. The intramolecular C-H activation proceeds under mild reaction conditions and short reaction time via electrochemically generated amidyl radicals. The reaction has been scaled up to gram level showing its practicability and the synthetic utility and applicability of the protocol has been demonstrated by the direct one-step synthesis of the bioactive compound Phenaglaydon.

Ultrasensitive Electrochemical Determination of Phosphate in water by Hydrophilic TiO2 modified Glassy Carbon Electrodes

2021 ◽  
Author(s):  
Yan Jin ◽  
Tong QI ◽  
Yuqing Ge ◽  
Jin Chen ◽  
Li juan Liang ◽  
...  
Keyword(s):  
Differential Pulse Voltammetry ◽  
Glassy Carbon ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Carbon Electrodes ◽  
Glassy Carbon Electrodes ◽  
Time Differential ◽  
Pulse Voltammetry ◽  
First Time

In this paper, ultrasensitive electrochemical determination of phosphate in water is achieved by hydrophilic TiO2 modified glassy carbon electrodes for the first time. Differential pulse voltammetry (DPV) method is proposed...

Polarographic Determination of Zinc in Compounded Rubber

1950 ◽  
Vol 23 (4) ◽  
pp. 975-980
Author(s):  
F. C. J. Poulton ◽  
L. Tarrant
Keyword(s):  
Zinc Oxide ◽  
Ammonium Acetate ◽  
Potassium Thiocyanate ◽  
Acetate Buffer Solution ◽  
Acid Electrolyte ◽  
Buffer Solution ◽  
Ammonium Acetate Buffer ◽  
Ammonium Acetate Buffer Solution ◽  
Gravimetric Procedure

Abstract Reasons are advanced for the unsatisfactory nature of some of the older methods for the determination of very small amounts of zinc in compounded rubber, particularly in latex mixings. The polarographic technique offers a possible solution, but most of the commoner electrolytes for the electroreduction of this metal are alkaline, and give rise to similar errors as are met in the gravimetric procedure. The development of a suitable acid electrolyte was therefore undertaken, and ways of dealing with likely interferences were examined. The electroltye finally recommended is a potassium thiocyanate-ammonium acetate buffer solution; iron, when present, is reduced to the ferrous condition by potassium iodide. The method was used to determine zinc oxide in a series of mixings of known composition ranging from 0.8 to 40 per cent. In all except the highest proportions of zinc oxide, the figures obtained agree well with the theoretical.

Electrochemical Determination of Uric Acid at CdTe Quantum Dot Modified Glassy Carbon Electrodes

2015 ◽  
Vol 98 (5) ◽  
pp. 1260-1266 ◽  
Author(s):  
Deng Pan ◽  
Shengzhong Rong ◽  
Guangteng Zhang ◽  
Yannan Zhang ◽  
Qiang Zhou ◽  
...  
Keyword(s):  
Uric Acid ◽  
Quantum Dot ◽  
Glassy Carbon ◽  
Semiconductor Nanocrystals ◽  
Phosphate Buffer Solution ◽  
Differential Pulse ◽  
Electrochemical Determination ◽  
Buffer Solution ◽  
Peak Current ◽  
Cdte Quantum Dot

Abstract Cyclic voltammetry and differential pulse voltammetry were used to investigate the electrochemical behavior of uric acid (UA) at a CdTe quantum dot (QD) modified the glassy carbon electrode (GCE). CdTe QDs, as new semiconductor nanocrystals, can greatly improve the peak current of UA. The anodic peak current of UA was linear with its concentration between 1.0 × 10–6 and 4.0 × 10–4 M in 0.1 M pH 5.0 phosphate buffer solution. The LOD for UA at the CdTe electrode (1.0 × 10–7 M) was superior to that of the GCE. In addition, we also determined the effects of scan rate, pH, and interferences of UA for the voltammetric behavior and detection. The results indicated that modified electrode possessed excellent reproducibility and stability. Finally, a new and efficient electrochemical sensor for detecting UA was developed.

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