Microfluidic Devices as Process Development Tools for Cellular Therapy Manufacturing

2021 ◽  
Author(s):  
Jorge Aranda Hernandez ◽  
Christopher Heuer ◽  
Janina Bahnemann ◽  
Nicolas Szita
Keyword(s):  
Cellular Therapy ◽  
Process Development ◽  
Microfluidic Devices ◽  
Development Tools

Systematic Product and Process Development Tools in Life Cycle Management

2020 ◽  
pp. 33-51
Author(s):  
Mohamad Taleuzzaman ◽  
Sanjay Chuhan ◽  
Sadaf Jamal Gilani ◽  
Syed Sarim Imam ◽  
Sarwar Beg
Keyword(s):  
Life Cycle ◽  
Process Development ◽  
Life Cycle Management ◽  
Development Tools ◽  
Product And Process

MEMS product engineering using fabrication process development tools

2008 ◽  
Author(s):  
K. Hahn ◽  
T. Schmidt ◽  
D. Ortloff ◽  
J. Popp ◽  
A. Wagener ◽  
...  
Keyword(s):  
Process Development ◽  
Fabrication Process ◽  
Development Tools ◽  
Product Engineering

scholarly journals Business process development with the application of simulation technique

2017 ◽  
Vol 2 (3) ◽  
pp. 109-118
Author(s):  
László Pusztai ◽  
Balázs Kocsi ◽  
István Budai
Keyword(s):  
Production Efficiency ◽  
Process Development ◽  
Data Gathering ◽  
Cost Effective ◽  
Diagnostic Methods ◽  
Small Enterprise ◽  
Process Time ◽  
Increase Production Efficiency ◽  
Development Tools ◽  
Long Run

The object of our work to increase production efficiency with the use of cost-efficient development tools at a Hungarian small enterprise. We apply data gathering techniques besides process modelling and diagnostic methods to reduce the total process time of the assembly process so as to induce cost reduction in the production. In this respect we investigate and rank root causes of wastes by cost-effective process development tools, like timebased FMEA (failure mode and effects analysis) as well as the 5 whys method. With the use of Monte-Carlo simulation companies can evaluate the change in the process, therefore, they can decide whether they want to carry out corrective measures or not. The accomplished changes can generate increasing competitive edge in the long run.

scholarly journals Designing Microfluidic Devices to Sort Haematopoietic Stem Cells Based on Their Mechanical Properties

2019 ◽  
Vol 2019 ◽  
pp. 1-13
Author(s):  
Mingming Du ◽  
Dean Kavanagh ◽  
Zhibing Zhang ◽  
Neena Kalia
Keyword(s):  
Mechanical Properties ◽  
Stem Cells ◽  
Cellular Therapy ◽  
Microfluidic Devices ◽  
High Flow ◽  
Cell Diameter ◽  
Label Free ◽  
Haematopoietic Stem Cells ◽  
Flow Rates

Aim. Few haematopoietic stem cells (HSCs) injected systemically for therapeutic purposes actually reach sites of injury as the vast majority become entrapped within pulmonary capillaries. One promising approach to maintain circulating HSC numbers would be to separate subpopulations with smaller size and/or greater deformability from a heterogeneous population. This study tested whether this could be achieved using label-free microfluidic devices.Methods. 2 straight (A-B) and 3 spiral (C-E) devices were fabricated with different dimensions. Cell sorting was performed at different flow rates after which cell diameter and stiffness were determined using micromanipulation. Cells isolated using the most efficient device were tested intravitally for their ability to home to the mouse injured gut.Results. Only straight Device B at a high flow rate separated HSCs with different mechanical properties. Side outlets collected mostly deformable cells (nominal rupture stress/σR=6.81 kPa; coefficient of variation/CV=0.31) at a throughput of2.3×105cells/min. All spiral devices at high flow rates separated HSCs with different stiffness and size. Inner outlets collected mostly deformable cells in Devices C (σR=25.06 kPa;CV=0.26), D (σR=22.21 kPa;CV=0.41), and E (σR=29.26 kPa;CV=0.27) at throughputs of2.3×105cells/min,1.5×105cells/min, and1.6×105cells/min, respectively. Since Device C separated cells with higher efficiency and throughput, it was utilized to test the homing ability of separated cellsin vivo. Significantly more deformable cells were observed trafficking through the injured gut—interestingly, increased retention was not observed.Conclusion. This study applied microfluidics to separate subpopulations from one stem cell type based on their intrinsic mechanical heterogeneity. Fluid dynamics within curved devices most effectively separated HSCs. Such devices may benefit cellular therapy.

Comparability study during manufacturing process development of Ovasave, an ovalbumin-specific type1 treg immuno-cellular therapy of refractory crohn’s Disease

Cytotherapy ◽  
2015 ◽  
Vol 17 (6) ◽  
pp. S20
Author(s):  
Nathalie Belmonte ◽  
Julie Gertner ◽  
Virginie Neveu ◽  
Nathalie Clerget ◽  
Valérie Brun ◽  
...  
Keyword(s):  
Crohn’S Disease ◽  
Crohn's Disease ◽  
Manufacturing Process ◽  
Cellular Therapy ◽  
Process Development

Advanced TEM sample preparation techniques for submicron Si devices

1995 ◽  
Vol 53 ◽  
pp. 516-517 ◽  
Author(s):  
P. B. Basham ◽  
H. L. Tsai
Keyword(s):  
Sample Preparation ◽  
Process Development ◽  
Light Transmission ◽  
Specific Area ◽  
Turnaround Time ◽  
Small Hole ◽  
Area Of Interest ◽  
Transmission Electron ◽  
Polished Side ◽  
Preparation Techniques

The use of transmission electron microscopy (TEM) to support process development of advanced microelectronic devices is often challenged by a large amount of samples submitted from wafer fabrication areas and specific-spot analysis. Improving the TEM sample preparation techniques for a fast turnaround time is critical in order to provide a timely support for customers and improve the utilization of TEM. For the specific-area sample preparation, a technique which can be easily prepared with the least amount of effort is preferred. For these reasons, we have developed several techniques which have greatly facilitated the TEM sample preparation.For specific-area analysis, the use of a copper grid with a small hole is found to be very useful. With this small-hole grid technique, TEM sample preparation can be proceeded by well-established conventional methods. The sample is first polished to the area of interest, which is then carefully positioned inside the hole. This polished side is placed against the grid by epoxy Fig. 1 is an optical image of a TEM cross-section after dimpling to light transmission.

The Structure and Properties of MoSi2 Thin Film in Mos Process

1980 ◽  
Vol 38 ◽  
pp. 326-327
Author(s):  
C.K. Wu ◽  
P. Chang ◽  
N. Godinho
Keyword(s):  
Thin Film ◽  
Integrated Circuits ◽  
High Performance ◽  
Large Scale ◽  
Process Development ◽  
Structure And Properties ◽  
Metal Silicides ◽  
High Oxidation ◽  
Important Approach ◽  
High Oxidation Resistance

Recently, the use of refractory metal silicides as low resistivity, high temperature and high oxidation resistance gate materials in large scale integrated circuits (LSI) has become an important approach in advanced MOS process development (1). This research is a systematic study on the structure and properties of molybdenum silicide thin film and its applicability to high performance LSI fabrication.

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