Strategies for Characterization of Enzymatic Nucleic Acids

2017 ◽  
pp. 37-58
Author(s):  
Fatemeh Javadi-Zarnaghi ◽  
Claudia Höbartner
Keyword(s):  
Nucleic Acids

Synthesis, Modification, and Characterization of Nanocarbon Electrodes for Determination of Nucleic Acids

2015 ◽  
pp. 241-281
Author(s):  
David Hynek ◽  
Katerina Tmejova ◽  
Jiri Kudr ◽  
Ondrej Zitka ◽  
Lukas Nejdl ◽  
...  
Keyword(s):  
Nucleic Acids

scholarly journals Calculation of Measurement Uncertainty in Quantitative Analysis of Genetically Modified Organisms Using Intermediate PrecisionA Practical Approach

2007 ◽  
Vol 90 (2) ◽  
pp. 582-586 ◽  
Author(s):  
Jana Žel ◽  
Kristina Gruden ◽  
Katarina Cankar ◽  
Dejan tebih ◽  
Andrej Blejec
Keyword(s):  
Nucleic Acids ◽  
Measurement Uncertainty ◽  
Genetically Modified Organisms ◽  
Genetically Modified ◽  
Laboratory Diagnostics ◽  
Quantitative Characterization ◽  
Intermediate Precision ◽  
Food And Feed ◽  
Repeatability And Reproducibility

Abstract Quantitative characterization of nucleic acids is becoming a frequently used method in routine analysis of biological samples, one use being the detection of genetically modified organisms (GMOs). Measurement uncertainty is an important factor to be considered in these analyses, especially where precise thresholds are set in regulations. Intermediate precision, defined as a measure between repeatability and reproducibility, is a parameter describing the real situation in laboratories dealing with quantitative aspects of molecular biology methods. In this paper, we describe the top-down approach to calculating measurement uncertainty, using intermediate precision, in routine GMO testing of food and feed samples. We illustrate its practicability in defining compliance of results with regulations. The method described is also applicable to other molecular methods for a variety of laboratory diagnostics where quantitative characterization of nucleic acids is needed.

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