Additions and Corrections - Radiation Chemistry of Nucleic Acids. Isolation and Characterization of Thymine Glycols.

1973 ◽  
Vol 95 (9) ◽  
pp. 3082-3082
Author(s):  
Bo-Sup. Hahn ◽  
Shih Yi. Wang
Keyword(s):  
Nucleic Acids ◽  
Radiation Chemistry ◽  
Isolation And Characterization

scholarly journals Biochemical genetics of Neurospora nuclease I: Isolation and characterization of nuclease (nuc) mutants

1983 ◽  
Vol 41 (3) ◽  
pp. 271-286 ◽  
Author(s):  
A. M. Forsthoefel ◽  
N. C. Mishra
Keyword(s):  
Nucleic Acids ◽  
Type Strain ◽  
Wild Type Strain ◽  
Extracellular Enzymes ◽  
Spontaneous Mutation ◽  
Subsequent Paper ◽  
Wild Type ◽  
Isolation And Characterization ◽  
Phosphorus Source

SUMMARYIsolation and characterization of five new nuclease (nuc) deficient mutants ofNeurosporahave been described. The new mutants are unable to utilize nucleic acids as the sole phosphorus source and possess growth characteristics similar to thosenuc(nuc-1andnuc-2) mutants described previously. Two new mutants (nuc-4andnuc-5) were able to use RNA or predigested DNA (but not intact DNA) as phosphorus source and showed temperature sensitive growth at 37 °C. Based on the data from complementation and genetic analyses the five new nuc mutants (nuc-3, nuc-4, nuc-5, nuc-6andnuc-7) were found nonallelic to each other and to previously describednuc(nuc-1andnuc-2) mutants; the newnucmutants mapped to the right ofarg-12on linkage group II. On biochemical analyses, thesenucmutants were found to possess a lower level of extracellular nucleases and alkaline phosphatase as compared to the wild type strain. The ds DNase activity of the new mutants was only about 2–12% of that of the wild type strain; thus, the low level of these extracellular enzymes in thenucmutants causes their inability to utilize nucleic acids as the sole phosphorus source. Wild type levels of these enzymes were restored in the complementing heterokaryons capable of full growth on the DNA medium. Data from intercrosses, mutagen sensitivity and spontaneous mutation-frequency studies (as discussed in a subsequent paper) indicated the involvement of thenucgenes in DNA repair and recombination.

The isolation and characterization of 18 equine microsatellite loci, TKY272–TKY289

2000 ◽  
Vol 31 (2) ◽  
pp. 149-149 ◽  
Author(s):  
T Tozaki ◽  
H Kakoi ◽  
S Mashima ◽  
K Hirota ◽  
T Hasegawa ◽  
...  
Keyword(s):  
Microsatellite Loci ◽  
Isolation And Characterization
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