scholarly journals Optimization of Insect Cell Based Protein Production Processes - Online Monitoring, Expression Systems, Scale Up

2013 ◽  
pp. 65-100 ◽  
Author(s):  
Damir Druzinec ◽  
Denise Salzig ◽  
Alexander Brix ◽  
Matthias Kraume ◽  
Andreas Vilcinskas ◽  
...  
Keyword(s):  
Insect Cell ◽  
Protein Production ◽  
Online Monitoring ◽  
Scale Up ◽  
Expression Systems ◽  
Production Processes

Protein production using the baculovirus-insect cell expression system

2013 ◽  
Vol 30 (1) ◽  
pp. 1-18 ◽  
Author(s):  
A. Contreras-Gómez ◽  
A. Sánchez-Mirón ◽  
F. García-Camacho ◽  
E. Molina-Grima ◽  
Y. Chisti
Keyword(s):  
Insect Cell ◽  
Protein Production ◽  
Expression System ◽  
Insect Cell Expression System ◽  
Cell Expression ◽  
Insect Cell Expression ◽  
Cell Expression System

Construction of gene expression systems in insect cell lines using promoters from the silkworm, Bombyx mori

2008 ◽  
Vol 133 (1) ◽  
pp. 9-17 ◽  
Author(s):  
Jae Man Lee ◽  
Masateru Takahashi ◽  
Hiroaki Mon ◽  
Hitoshi Mitsunobu ◽  
Katsumi Koga ◽  
...  
Keyword(s):  
Gene Expression ◽  
Bombyx Mori ◽  
Cell Lines ◽  
Insect Cell ◽  
Expression Systems ◽  
Insect Cell Lines ◽  
Silkworm Bombyx Mori

Intermediates of Open Innovation in the Aquaculture Industry

2019 ◽  
pp. 203-222 ◽  
Author(s):  
Jorge Ramos ◽  
Pedro Pousão Ferreira
Keyword(s):  
Information Technology ◽  
Knowledge Transfer ◽  
Open Innovation ◽  
Protein Production ◽  
Cultural Factors ◽  
Know How ◽  
Governmental Organizations ◽  
Production Processes ◽  
Non Governmental Organizations ◽  
Social And Cultural Factors

Aquaculture appears to be the most adequate and logical way to suit the protein production problem. Basically, because unlike fisheries that are highly unpredictable, aquaculture rearing is based upon production protocols. This chapter developed an argument supporting the above rationale. As it stands, the evidence suggests that with the current use of information technology, it becomes a common practice to exchange aquaculture knowledge between firms, research bodies, or non-governmental organizations. The interaction between the whole set of stakeholders, where a broad range of know-how and intermediate products and services are developed, generates innovation. Sharing know-how creates open innovation. Open innovation, by its turn, seeks efficiency and generates more sustainable production processes. The way knowledge transfer is delivered and its trends are dependent upon dimensions such as economic, social, and cultural factors.

scholarly journals Aliivibrio wodanis as a production host: development of genetic tools for expression of cold-active enzymes

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Jenny Johansson Söderberg ◽  
Miriam Grgic ◽  
Erik Hjerde ◽  
Peik Haugen
Keyword(s):  
Low Temperature ◽  
Protein Production ◽  
Fluorescent Protein ◽  
Active Form ◽  
Rhodococcus Erythropolis ◽  
Expression Systems ◽  
Cold Adapted ◽  
Genetic Tools ◽  
Cold Active Enzymes ◽  
Cold Active

Abstract Background Heterologous production of cold-adapted proteins currently represents one of the greatest bottlenecks in the ongoing bioprospecting efforts to find new enzymes from low-temperature environments, such as, the polar oceans that represent essentially untapped resources in this respect. In mesophilic expression hosts such as Escherichia coli, cold-adapted enzymes often form inactive aggregates. Therefore it is necessary to develop new low-temperature expression systems, including identification of new host organisms and complementary genetic tools. Psychrophilic bacteria, including Pseudoalteromonas haloplanktis, Shewanella and Rhodococcus erythropolis have all been explored as candidates for such applications. However to date none of these have found widespread use as efficient expression systems, or are commercially available. In the present work we explored the use of the sub-Arctic bacterium Aliivibrio wodanis as a potential host for heterologous expression of cold-active enzymes. Results We tested 12 bacterial strains, as well as available vectors, promoters and reporter systems. We used RNA-sequencing to determine the most highly expressed genes and their intrinsic promoters in A. wodanis. In addition we examined a novel 5′-fusion to stimulate protein production and solubility. Finally we tested production of a set of “difficult-to-produce” enzymes originating from various bacteria and one Archaea. Our results show that cold-adapted enzymes can be produced in soluble and active form, even in cases when protein production failed in E. coli due to the formation of inclusion bodies. Moreover, we identified a 60-bp/20-aa fragment from the 5′-end of the AW0309160_00174 gene that stimulates expression of Green Fluorescent Protein and improves production of cold-active enzymes when used as a 5′-fusion. A 25-aa peptide from the same protein enhanced secretion of a 25-aa-sfGFP fusion. Conclusions Our results indicate the use of A. wodanis and associated genetic tools for low-temperature protein production and indicate that A. wodanis represents an interesting platform for further development of a protein production system that can promote further cold-enzyme discoveries.

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