scholarly journals Production and Purification of the Heavy Chain Fragment C of Botulinum Neurotoxin, Serotype A, Expressed in the Methylotrophic Yeast Pichia pastoris

2000 ◽  
Vol 19 (3) ◽  
pp. 393-402 ◽  
Author(s):  
Karen J. Potter ◽  
Wenhui Zhang ◽  
Leonard A. Smith ◽  
Michael M. Meagher
Keyword(s):  
Pichia Pastoris ◽  
Heavy Chain ◽  
Botulinum Neurotoxin ◽  
Methylotrophic Yeast ◽  
Chain Fragment ◽  
Yeast Pichia Pastoris ◽  
Serotype A ◽  
Methylotrophic Yeast Pichia Pastoris ◽  
Botulinum Neurotoxin Serotype A

The lipidome and proteome of microsomes from the methylotrophic yeast Pichia pastoris

2014 ◽  
Vol 1841 (2) ◽  
pp. 215-226 ◽  
Author(s):  
Lisa Klug ◽  
Pablo Tarazona ◽  
Clemens Gruber ◽  
Karlheinz Grillitsch ◽  
Brigitte Gasser ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Methylotrophic Yeast ◽  
Yeast Pichia Pastoris ◽  
Methylotrophic Yeast Pichia Pastoris

scholarly journals Positive Selection of Novel Peroxisome Biogenesis-Defective Mutants of the Yeast Pichia pastoris

Genetics ◽  
1999 ◽  
Vol 151 (4) ◽  
pp. 1379-1391
Author(s):  
Monique A Johnson ◽  
Hans R Waterham ◽  
Galyna P Ksheminska ◽  
Liubov R Fayura ◽  
Joan Lin Cereghino ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Allyl Alcohol ◽  
Alcohol Oxidase ◽  
Methylotrophic Yeast ◽  
Direct Selection ◽  
Toxic Exposure ◽  
Peroxisome Biogenesis ◽  
Yeast Pichia Pastoris ◽  
Methylotrophic Yeast Pichia Pastoris ◽  
Selection Of

Abstract We have developed two novel schemes for the direct selection of peroxisome-biogenesis-defective (pex) mutants of the methylotrophic yeast Pichia pastoris. Both schemes take advantage of our observation that methanol-induced pex mutants contain little or no alcohol oxidase (AOX) activity. AOX is a peroxisomal matrix enzyme that catalyzes the first step in the methanol-utilization pathway. One scheme utilizes allyl alcohol, a compound that is not toxic to cells but is oxidized by AOX to acrolein, a compound that is toxic. Exposure of mutagenized populations of AOX-induced cells to allyl alcohol selectively kills AOX-containing cells. However, pex mutants without AOX are able to grow. The second scheme utilizes a P. pastoris strain that is defective in formaldehyde dehydrogenase (FLD), a methanol pathway enzyme required to metabolize formaldehyde, the product of AOX. AOX-induced cells of fld1 strains are sensitive to methanol because of the accumulation of formaldehyde. However, fld1 pex mutants, with little active AOX, do not efficiently oxidize methanol to formaldehyde and therefore are not sensitive to methanol. Using these selections, new pex mutant alleles in previously identified PEX genes have been isolated along with mutants in three previously unidentified PEX groups.

scholarly journals Binding activity and immunogenic characterization of recombinant C-terminal quarter and half of the heavy chain of botulinum neurotoxin serotype A

2011 ◽  
Vol 7 (10) ◽  
pp. 1090-1095 ◽  
Author(s):  
Yun-Zhou Yu ◽  
Yao Ma ◽  
Yan-Xia Chen ◽  
Zheng-Wei Gong ◽  
Shuang Wang ◽  
...  
Keyword(s):  
Heavy Chain ◽  
Botulinum Neurotoxin ◽  
Binding Activity ◽  
Serotype A ◽  
Botulinum Neurotoxin Serotype A

scholarly journals Lipidome and proteome of lipid droplets from the methylotrophic yeast Pichia pastoris

2013 ◽  
Vol 1831 (2) ◽  
pp. 282-290 ◽  
Author(s):  
Vasyl A. Ivashov ◽  
Karlheinz Grillitsch ◽  
Harald Koefeler ◽  
Erich Leitner ◽  
Dominic Baeumlisberger ◽  
...  
Keyword(s):  
Pichia Pastoris ◽  
Lipid Droplets ◽  
Methylotrophic Yeast ◽  
Yeast Pichia Pastoris ◽  
Methylotrophic Yeast Pichia Pastoris
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