Expression and Purification of a Synthetic Human Obese Gene Product

1995 ◽  
Vol 6 (6) ◽  
pp. 722-726 ◽  
Author(s):  
Scott W. Altmann ◽  
Jackie C. Timans ◽  
Fernando L. Rock ◽  
J.Fernando Bazan ◽  
Robert A. Kastelein
Keyword(s):  
Gene Product ◽  
Expression And Purification ◽  
Obese Gene

scholarly journals The Adipose Obese Gene Product, Leptin: Evidence of a Direct Inhibitory Role in Ovarian Function*

Endocrinology ◽  
1997 ◽  
Vol 138 (8) ◽  
pp. 3374-3379 ◽  
Author(s):  
Leon J. Spicer ◽  
Cristina C. Francisco
Keyword(s):  
Gene Product ◽  
Ovarian Function ◽  
Obese Gene ◽  
Inhibitory Role

scholarly journals Screening of Microbial Products Modifying the Action of Leptin (Obese Gene Product) by a Biosensor.

1999 ◽  
Vol 52 (4) ◽  
pp. 429-432 ◽  
Author(s):  
TOMOMI HIDAKA ◽  
TAKEHIKO MORISHITA ◽  
KUMIKO W. SHIMOTOHNO ◽  
TOYOSHIGE ENDO ◽  
HARUO SETO ◽  
...  
Keyword(s):  
Gene Product ◽  
Obese Gene ◽  
Microbial Products

Effects of the obese gene product on body weight regulation in ob/ob mice

Science ◽  
1995 ◽  
Vol 269 (5223) ◽  
pp. 540-543 ◽  
Author(s):  
M. Pelleymounter ◽  
M. Cullen ◽  
M. Baker ◽  
R Hecht ◽  
D Winters ◽  
...  
Keyword(s):  
Body Weight ◽  
Gene Product ◽  
Weight Regulation ◽  
Body Weight Regulation ◽  
Obese Gene

scholarly journals Adipose Obese Gene Product, Leptin, Inhibits Bovine Ovarian Thecal Cell Steroidogenesis1

1998 ◽  
Vol 58 (1) ◽  
pp. 207-212 ◽  
Author(s):  
Leon J. Spicer ◽  
Cristina C. Francisco
Keyword(s):  
Gene Product ◽  
Thecal Cell ◽  
Obese Gene

scholarly journals Clinical Implication of Leptin, the Obese Gene Product.

1999 ◽  
Vol 38 (2) ◽  
pp. 210-212 ◽  
Author(s):  
Yoshihiro OGAWA ◽  
Hiroaki MASUZAKI ◽  
Kazuwa NAKAO
Keyword(s):  
Gene Product ◽  
Clinical Implication ◽  
Obese Gene

scholarly journals Expression and purification of the first nucleotide-binding domain and linker region of human multidrug resistance gene product: comparison of fusions to glutathione S-transferase, thioredoxin and maltose-binding protein

1999 ◽  
Vol 338 (1) ◽  
pp. 77-81 ◽  
Author(s):  
Changsen WANG ◽  
Ariel F. CASTRO ◽  
Denise M. WILKES ◽  
Guillermo A. ALTENBERG
Keyword(s):  
Multidrug Resistance ◽  
Resistance Gene ◽  
Gene Product ◽  
Nucleotide Binding ◽  
Maltose Binding Protein ◽  
Nucleotide Binding Domain ◽  
Expression And Purification ◽  
Glutathione S Transferase ◽  
Multidrug Resistance Gene ◽  
Linker Region

Many membrane proteins that belong to the ATP-binding cassette (ABC) superfamily are clinically important, including the cystic fibrosis transmembrane conductance regulator, the sulphonylurea receptor and P-glycoprotein (multidrug resistance gene product; MDR1). These proteins contain two multispanning transmembrane domains, each followed by one nucleotide-binding domain (NBD) and a linker region distal to the first NBD. ATP hydrolysis by the NBDs is critical for ABC protein function; the linker region seems to have a regulatory role. Previous attempts to express soluble NBDs and/or linker regions without detergent solubilization, or to purify NBDs at high yields as soluble fusion proteins, have been unsuccessful. Here we present a system for the expression in Escherichia coli of the first NBD of MDR1 followed by its linker region (NBD1MLD). A comparison of the expressions of NBD1MLD fused to glutathione S-transferase, thioredoxin and maltose-binding protein (MBP) shows that a high level of expression in the soluble fraction (approx. 8% of total E. coli protein) can be achieved only for MBP–NBD1MLD. The addition of a proteolytic thrombin site just proximal to the N-terminal end of NBD1MLD allows the cleavage of NBD1MLD from MBP, which can be easily purified with retention of its ATPase activity. In summary, success was obtained only when using an MBP fusion protein vector containing a thrombin proteolytic site between MBP and NBD1MLD. The approach described here could be generally applicable to solving the problems of expression and purification of NBDs/linker regions of ABC proteins.

The obese gene product, leptin

1998 ◽  
Vol 16 (Supplement) ◽  
pp. 2007-2012 ◽  
Author(s):  
Hiroshi Hirose ◽  
Ikuo Saito ◽  
Minako Tsujioka ◽  
Masaaki Mori ◽  
Hiroshi Kawabe ◽  
...  
Keyword(s):  
Gene Product ◽  
Obese Gene

Threading analysis suggests that the obese gene product may be a helical cytokine

FEBS Letters ◽  
1995 ◽  
Vol 373 (1) ◽  
pp. 13-18 ◽  
Author(s):  
Thomas Madej ◽  
Mark S. Boguski ◽  
Stephen H. Bryant
Keyword(s):  
Gene Product ◽  
Obese Gene
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