Milsana-induced resistance in powdery mildew-infected cucumber plants correlates with the induction of chalcone synthase and chalcone isomerase

2002 ◽  
Vol 61 (2) ◽  
pp. 121-132 ◽  
Author(s):  
Bourlaye Fofana ◽  
David J McNally ◽  
Caroline Labbé ◽  
Raynald Boulanger ◽  
Nicole Benhamou ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Induced Resistance ◽  
Chalcone Synthase ◽  
Chalcone Isomerase

Purification of Chalcone Synthase from Tulip Anthers and Comparison with the Synthase from Cosmos Petals

1981 ◽  
Vol 36 (1-2) ◽  
pp. 30-34 ◽  
Author(s):  
Rainer Sütfeld ◽  
Rolf Wiermann
Keyword(s):  
Chalcone Synthase ◽  
In Vitro Assay ◽  
Flavonoid Biosynthesis ◽  
Chalcone Isomerase ◽  
Gel Chromatography ◽  
Purification Procedure ◽  
Reaction Products ◽  
Complete Elimination ◽  
Vitro Assay

Abstract Chalcone synthase was isolated from both anthers of Tulipa cv. “Apeldoorn” and petals of Cosmos sulphureus Cav. After certain prepurification steps, the enzymes were further purified using gel chromatography on Sephadex G-200 followed by repeated hydroxylapatite absorption chromatography. Both the enzymes showed the same chromatographic properties. After gel chromatography as well as after the first hydroxylapatite fractionation, the reaction products appeared as flavanones. However, after the second hydroxylapatite step, production of chalcones was observed. Like the enzyme from tulip anthers, the synthase from Cosmos petals produced the correspondingly substituted chalcones when p-coumaroyl-CoA, caffeoyl-CoA and feruloyl-CoA, respectively, were used as substractes. In both the cases, the ratios of the different chalcones produced were found to be about the same. The appearance of chalcone synthesis in this in vitro assay is caused by the complete elimination of chalcone isomerase in the purification procedure. The importance of the isomerase for flavonoid biosynthesis, particularly in plant systems which are accumulating chalcones, is discussed.

Heat-induced resistance in barley to the powdery mildew fungus Erysiphe graminis f.sp. hordei

1995 ◽  
Vol 47 (1) ◽  
pp. 51-66 ◽  
Author(s):  
Patrick Schweizer ◽  
Laurence Vallélian-Bindschedler ◽  
Egon Mösinger
Keyword(s):  
Powdery Mildew ◽  
Induced Resistance ◽  
Erysiphe Graminis ◽  
Powdery Mildew Fungus

scholarly journals Unraveling the Biochemical Base of Dahlia Flower Coloration

2008 ◽  
Vol 3 (8) ◽  
pp. 1934578X0800300 ◽  
Author(s):  
Heidi Halbwirth ◽  
Gerlinde Muster ◽  
Karl Stich
Keyword(s):  
Chalcone Synthase ◽  
Flower Color ◽  
Chalcone Isomerase ◽  
Anthocyanin Content ◽  
Anthocyanin Accumulation ◽  
Enzyme Preparations ◽  
Yellow Color ◽  
Flavone Synthase ◽  
Flavone Synthase Ii ◽  
Related Compounds

Dahlia ( Dahlia variabilis) exists in a dazzling array of cultivars, showing red, orange, magenta, lilac, yellow and white flower color, which is exclusively based on the presence of flavonoids and biochemically related compounds. Red hues (red, orange, magenta, lilac) are a result of anthocyanin accumulation in varying concentration and composition, while a yellow color is based on the formation of 6′-deoxychalcones in the petals. Red dahlia pigments are all derived from pelargonidin and cyanidin. Delphinidin derivatives are not formed due to the absence of flavonoid 3′,5′-hydroxylase in dahlia petals, which provides an explanation for the lack of blue dahlia flowers. Orange, lilac and rose cultivars are characterized by a lower anthocyanin content compared to many red cultivars. We investigated 198 cultivars for the presence of flavonoid enzymes. The activities of chalcone isomerase (CHI), chalcone synthase (CHS), dihydroflavonol 4-reductase (DFR), flavanone 3-hydroxylase (FHT), flavone synthase II (FNSII), flavonol synthase (FLS) and flavonoid 3′-hydroxylase (F3′H) were demonstrated in enzyme preparations of dahlia petals. CHI accepted 6′-hydroxychalcones as substrates, but did not catalyze the conversion of 6′-deoxychalcones to the corresponding flavanones. White cultivars were frequently characterized by the lack of DFR activity, whereas in many yellow cultivars neither FHT nor DFR activity could be shown.

Chemical-induced resistance against powdery mildew in barley: the effects of chitosan and benzothiadiazole

BioControl ◽  
2007 ◽  
Vol 53 (2) ◽  
pp. 387-401 ◽  
Author(s):  
Franco Faoro ◽  
Dario Maffi ◽  
Dario Cantu ◽  
Marcello Iriti
Keyword(s):  
Powdery Mildew ◽  
Induced Resistance

Silicon induced resistance against powdery mildew of roses caused by Podosphaera pannosa

2011 ◽  
Vol 61 (1) ◽  
pp. 120-131 ◽  
Author(s):  
R. Shetty ◽  
B. Jensen ◽  
N. P. Shetty ◽  
M. Hansen ◽  
C. W. Hansen ◽  
...  
Keyword(s):  
Powdery Mildew ◽  
Induced Resistance
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