Using Inducible Vectors to Study Intracellular Trafficking of GFP-Tagged Steroid/Nuclear Receptors in Living Cells

Methods ◽  
1999 ◽  
Vol 19 (3) ◽  
pp. 386-393 ◽  
Author(s):  
Dawn Walker ◽  
Han Htun ◽  
Gordon L. Hager
Keyword(s):  
Nuclear Receptors ◽  
Intracellular Trafficking ◽  
Living Cells

Intracellular trafficking and cytoplasmic streaming under abiotic stress conditions

2019 ◽  
Vol 6 (04) ◽  
Author(s):  
JESHIMA KHAN YASIN ◽  
ANIL KUMAR SINGH
Keyword(s):  
Plasma Membrane ◽  
Abiotic Stress ◽  
Confocal Microscopy ◽  
Fusion Protein ◽  
Intracellular Trafficking ◽  
Cytoplasmic Streaming ◽  
Living Cells ◽  
Stress Conditions ◽  
Vesicle Formation ◽  
External Stimuli

Cytoplasmic streaming is one among the vital activities of the living cells. In plants cytolplasmic streaming could clearly be seen in hypocotyls of growing seedlings. To observe cytoplsmic streaming and its correlated intracellular trafficking an investigation was conducted in legumes in comparison with GFP-AtRab75 and 35S::GFP:δTIP tonoplast fusion protein expressing arabidopsis lines. These seedlings were observed under confocal microscopy with different buffer incubation treatments and under different stress conditions. GFP expressing 35S::GFP:δTIP tonoplast lines were looking similar to the control lines and differ under stress conditions. Movement of cytoplasmic invaginations within the tonoplast and cytoplasmic sub vesicle or bulb budding during cytoplasmic streaming was observed in hypocotyls of At-GFP tonoplast plants. We found the cytoplasmic bulbs/ vesicles or sub vesicle formation from the plasma membrane. The streaming speed also depends on the incubation medium in which the specimen was incubated, indicating that the external stimuli as well as internal stimuli can alter the speed of streaming

Efficient and High-Speed Transduction of an Antibody into Living Cells Using a Multifunctional Nanocarrier System to Control Intracellular Trafficking

2015 ◽  
Vol 104 (9) ◽  
pp. 2845-2854 ◽  
Author(s):  
Yuma Yamada ◽  
Sandra Milena Vergara Perez ◽  
Mai Tabata ◽  
Jiro Abe ◽  
Yukari Yasuzaki ◽  
...  
Keyword(s):  
Intracellular Trafficking ◽  
High Speed ◽  
Living Cells

Trafficking of nuclear receptors in living cells

2000 ◽  
Vol 74 (5) ◽  
pp. 249-254 ◽  
Author(s):  
Gordon L Hager ◽  
Carol S Lim ◽  
Cem Elbi ◽  
Christopher T Baumann
Keyword(s):  
Nuclear Receptors ◽  
Living Cells

Dendrimer Internalization and Intracellular Trafficking in Living Cells

2010 ◽  
Vol 7 (3) ◽  
pp. 680-688 ◽  
Author(s):  
Lorenzo Albertazzi ◽  
Michela Serresi ◽  
Alberto Albanese ◽  
Fabio Beltram
Keyword(s):  
Intracellular Trafficking ◽  
Living Cells

scholarly journals Intracellular trafficking of cationic liposome–DNA complexes in living cells

Soft Matter ◽  
2012 ◽  
Vol 8 (30) ◽  
pp. 7919 ◽  
Author(s):  
Stefano Coppola ◽  
Laura C. Estrada ◽  
Michelle A. Digman ◽  
Daniela Pozzi ◽  
Francesco Cardarelli ◽  
...  
Keyword(s):  
Intracellular Trafficking ◽  
Cationic Liposome ◽  
Living Cells ◽  
Dna Complexes

scholarly journals Perturbing the Normal Level of SIDT1 Suppresses the Naked ASO Effect

2021 ◽  
Vol 2021 ◽  
pp. 1-9
Author(s):  
Masayuki Takahashi ◽  
Mineaki Seki ◽  
Masayuki Nashimoto ◽  
Tomohiro Kabuta
Keyword(s):  
Flow Cytometry ◽  
Cellular Uptake ◽  
Normal Level ◽  
Antisense Oligonucleotide ◽  
Intracellular Trafficking ◽  
Microscopic Analysis ◽  
Living Cells ◽  
Therapeutic Effects ◽  
Fluorescence Microscopic Analysis ◽  
Antisense Effect

Although antisense oligonucleotide (ASO) therapeutics can be taken up by living cells without carrier molecules, a large part of incorporated ASOs are trapped in the endosomes and do not exert therapeutic effects. To improve their therapeutic effects, it would be important to elucidate the mechanism of cellular uptake and intracellular trafficking of ASOs. In this study, we investigated how SIDT1 affects cellular uptake and intracellular trafficking of ASOs. Fluorescence microscopic analysis suggested that most of naked ASOs are trafficked to the lysosomes via the endosomes. The data obtained from flow cytometry and fluorescence microscopy together showed that although the SIDT1 level barely affects the total cellular uptake of ASOs, it appears to affect the intracellular trafficking of ASOs. We also showed that SIDT1 exists mainly in the endoplasmic reticulum and that perturbing the normal level of SIDT1 suppresses the antisense effect of the naked ASO targeting miR-16.

FRAP and FRET Methods to Study Nuclear Receptors in Living Cells

2009 ◽  
pp. 69-96 ◽  
Author(s):  
Martin E. Royen ◽  
Christoffel Dinant ◽  
Pascal Farla ◽  
Jan Trapman ◽  
Adriaan B. Houtsmuller
Keyword(s):  
Nuclear Receptors ◽  
Living Cells

scholarly journals Analysis of Intracellular Trafficking and Interactions of Cytoplasmic HIV-1 Rev Mutants in Living Cells

Virology ◽  
1998 ◽  
Vol 251 (1) ◽  
pp. 38-48 ◽  
Author(s):  
Roland H. Stauber ◽  
Elena Afonina ◽  
Sergei Gulnik ◽  
John Erickson ◽  
George N. Pavlakis
Keyword(s):  
Intracellular Trafficking ◽  
Living Cells ◽  
Hiv 1
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