Identification of Escherichia coli O157:H7 and other enterohemorrhagic serotypes by EHEC- hly A targeting, strand displacement amplification, and fluorescence polarization

2002 ◽  
Vol 16 (2) ◽  
pp. 85-92 ◽  
Author(s):  
B Ge ◽  
C Larkin ◽  
S Ahn ◽  
M Jolley ◽  
M Nasir ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Fluorescence Polarization ◽  
Escherichia Coli O157 ◽  
Strand Displacement ◽  
Strand Displacement Amplification

scholarly journals Detection of Mycobacterium tuberculosis DNA with thermophilic strand displacement amplification and fluorescence polarization

1996 ◽  
Vol 42 (10) ◽  
pp. 1604-1608 ◽  
Author(s):  
G T Walker ◽  
C P Linn
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Restriction Enzyme ◽  
Fluorescence Polarization ◽  
Target Sequence ◽  
Strand Displacement ◽  
Insertion Element ◽  
Strand Displacement Amplification ◽  
Simple Protocol ◽  
Polymerase Binding

Abstract Strand displacement amplification (SDA) is an isothermal, in vitro method for diagnostics that amplifies a target DNA sequence by using a restriction enzyme and DNA polymerase. We have combined a new thermophilic form of SDA that involves restriction enzyme BsoBI and polymerase exo-Bca with fluorescence polarization for detection of Mycobacterium tuberculosis DNA by using the IS6110 insertion element as the target sequence. A 5'-fluorescein-labeled oligodeoxynucleotide detector probe hybridizes to the amplified product as it rises in concentration during SDA, and the single- to double-stranded conversion is monitored through an increase in fluorescence polarization. The associated change in polarization upon amplification of the target sequence is enhanced by specific polymerase binding to the double-stranded detector probe. Fewer than 10 M. tuberculosis genomes can be amplified and detected with an extremely simple protocol that takes only 20 min and uses relatively simple instrumentation and reagents, all of which can be purchased off-the-shelf.

Visual assay of Escherichia coli O157:H7 based on isothermal strand displacement and hybrid chain reaction amplification strategy

2021 ◽  
Author(s):  
Liu Xiu ◽  
Shengjun Bu ◽  
Hongguo Wei ◽  
Wang Ze ◽  
Songling Yu ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
16S Rrna ◽  
Point Of Care ◽  
Escherichia Coli O157 ◽  
Strand Displacement ◽  
Chain Reaction ◽  
Reaction Amplification ◽  
Visual Assay ◽  
Amplification Strategy ◽  
Point Of Care Detection

Here, we describe a simple, sensitive, and enzyme-free method for visual point-of-care detection of 16S rRNA of Escherichia coli O157:H7 based on isothermal strand displacement-hybrid chain reaction (ISD-HCR) and lateral...

scholarly journals Strand displacement amplification (SDA) and transient-state fluorescence polarization detection of Mycobacterium tuberculosis DNA

1996 ◽  
Vol 42 (1) ◽  
pp. 9-13 ◽  
Author(s):  
G T Walker ◽  
J G Nadeau ◽  
C P Linn ◽  
R F Devlin ◽  
W B Dandliker
Keyword(s):  
Mycobacterium Tuberculosis ◽  
Dna Sequence ◽  
Fluorescence Polarization ◽  
Near Infrared ◽  
Detection System ◽  
Transient State ◽  
Strand Displacement ◽  
Strand Displacement Amplification ◽  
Polarization Detection

Abstract Strand displacement amplification (SDA) is an isothermal, in vitro method of amplifying a DNA sequence for diagnostic purposes. We have combined SDA with fluorescence polarization detection in a closed, homogeneous format. A fluorescently labeled oligodeoxynucleotide detector probe hybridizes to the amplification product that increases in concentration during SDA. The single- to double-stranded conversion of the probe is accompanied by an increase in fluorescence polarization values, which can be measured in real-time without physical manipulation of the sample. The probe was labeled with the near-infrared dye La Jolla Blue, and fluorescence polarization was measured on a transient-state fluorometer. We have applied this homogeneous SDA/detection system to a target DNA sequence specific for Mycobacterium tuberculosis DNA.

Attomolar Detection of Proteins via Cascade Strand-Displacement Amplification and Polystyrene Nanoparticle Enhancement in Fluorescence Polarization Aptasensors

2015 ◽  
Vol 87 (16) ◽  
pp. 8107-8114 ◽  
Author(s):  
Yong Huang ◽  
Xiaoqian Liu ◽  
Huakui Huang ◽  
Jian Qin ◽  
Liangliang Zhang ◽  
...  
Keyword(s):  
Fluorescence Polarization ◽  
Strand Displacement ◽  
Strand Displacement Amplification

Bedeutung von Escherichia coli O157 beim Schlachtschaf in der Schweiz

2006 ◽  
Vol 148 (6) ◽  
pp. 289-295 ◽  
Author(s):  
C. Zweifel ◽  
M. Kaufmann ◽  
J. Blanco ◽  
R. Stephan
Keyword(s):  
Escherichia Coli ◽  
Escherichia Coli O157
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