scholarly journals DNA detection by strand displacement amplification and fluorescence polarization with signal enhancement using a DNA binding protein

1996 ◽  
Vol 24 (2) ◽  
pp. 348-353 ◽  
Author(s):  
G. Walker
Keyword(s):  
Dna Binding ◽  
Fluorescence Polarization ◽  
Dna Detection ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Signal Enhancement ◽  
Strand Displacement ◽  
Strand Displacement Amplification

scholarly journals In Vivo Occupancy of Mitochondrial Single-Stranded DNA Binding Protein Supports the Strand Displacement Mode of DNA Replication

PLoS Genetics ◽  
2014 ◽  
Vol 10 (12) ◽  
pp. e1004832 ◽  
Author(s):  
Javier Miralles Fusté ◽  
Yonghong Shi ◽  
Sjoerd Wanrooij ◽  
Xuefeng Zhu ◽  
Elisabeth Jemt ◽  
...  
Keyword(s):  
Dna Replication ◽  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Strand Displacement ◽  
Single Stranded Dna ◽  
Displacement Mode

scholarly journals Purification of Host Cell Enzymes Involved in Adeno-Associated Virus DNA Replication

2007 ◽  
Vol 81 (11) ◽  
pp. 5777-5787 ◽  
Author(s):  
Kevin Nash ◽  
Weijun Chen ◽  
William F. McDonald ◽  
Xiaohuai Zhou ◽  
Nicholas Muzyczka
Keyword(s):  
Dna Replication ◽  
Dna Binding ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Dna Helicase ◽  
Strand Displacement ◽  
Adeno Associated Virus ◽  
Replication System ◽  
Factor C

ABSTRACT Adeno-associated virus (AAV) replicates its DNA by a modified rolling-circle mechanism that exclusively uses leading strand displacement synthesis. To identify the enzymes directly involved in AAV DNA replication, we fractionated adenovirus-infected crude extracts and tested them in an in vitro replication system that required the presence of the AAV-encoded Rep protein and the AAV origins of DNA replication, thus faithfully reproducing in vivo viral DNA replication. Fractions that contained replication factor C (RFC) and proliferating cell nuclear antigen (PCNA) were found to be essential for reconstituting AAV DNA replication. These could be replaced by purified PCNA and RFC to retain full activity. We also found that fractions containing polymerase δ, but not polymerase ε or α, were capable of replicating AAV DNA in vitro. This was confirmed when highly purified polymerase δ complex purified from baculovirus expression clones was used. Curiously, as the components of the DNA replication system were purified, neither the cellular single-stranded DNA binding protein (RPA) nor the adenovirus-encoded DNA binding protein was found to be essential for DNA replication; both only modestly stimulated DNA synthesis on an AAV template. Also, in addition to polymerase δ, RFC, and PCNA, an as yet unidentified factor(s) is required for AAV DNA replication, which appeared to be enriched in adenovirus-infected cells. Finally, the absence of any apparent cellular DNA helicase requirement led us to develop an artificial AAV replication system in which polymerase δ, RFC, and PCNA were replaced with T4 DNA polymerase and gp32 protein. This system was capable of supporting AAV DNA replication, demonstrating that under some conditions the Rep helicase activity can function to unwind duplex DNA during strand displacement synthesis.

Single-Stranded DNA Binding Protein-Assisted Fluorescence Polarization Aptamer Assay for Detection of Small Molecules

2012 ◽  
Vol 84 (16) ◽  
pp. 7203-7211 ◽  
Author(s):  
Zhenyu Zhu ◽  
Corinne Ravelet ◽  
Sandrine Perrier ◽  
Valérie Guieu ◽  
Emmanuelle Fiore ◽  
...  
Keyword(s):  
Dna Binding ◽  
Small Molecules ◽  
Fluorescence Polarization ◽  
Binding Protein ◽  
Dna Binding Protein ◽  
Single Stranded Dna ◽  
Aptamer Assay

The DNA-binding protein YB-1 is a direct MYCN target and influences repair and resistance in neuroblastoma cell lines

2010 ◽  
Vol 222 (03) ◽  
Author(s):  
S Degen ◽  
S Kuhfittig-Kulle ◽  
JH Schulte ◽  
F Westermann ◽  
A Schramm ◽  
...  
Keyword(s):  
Dna Binding ◽  
Cell Lines ◽  
Binding Protein ◽  
Neuroblastoma Cell ◽  
Dna Binding Protein ◽  
Neuroblastoma Cell Lines
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