Lipopolysaccharide-Binding Protein Accelerates and Augments Escherichia coli Phagocytosis by Alveolar Macrophages

2000 ◽  
Vol 94 (2) ◽  
pp. 159-166 ◽  
Author(s):  
Richard D Klein ◽  
Grace L Su ◽  
Carl Schmidt ◽  
Alireza Aminlari ◽  
Lars Steinstraesser ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Alveolar Macrophages ◽  
Binding Protein ◽  
Lipopolysaccharide Binding Protein ◽  
Lipopolysaccharide Binding

Preparation and characterization of truncated human lipopolysaccharide-binding protein in Escherichia coli

2006 ◽  
Vol 49 (2) ◽  
pp. 276-283 ◽  
Author(s):  
Jun Kohara ◽  
Naoko Tsuneyoshi ◽  
Jean-François Gauchat ◽  
Masao Kimoto ◽  
Kenji Fukudome
Keyword(s):  
Escherichia Coli ◽  
Binding Protein ◽  
Lipopolysaccharide Binding Protein ◽  
Lipopolysaccharide Binding

scholarly journals Lactoferrin Inhibits the Endotoxin Interaction with CD14 by Competition with the Lipopolysaccharide-Binding Protein

1998 ◽  
Vol 66 (2) ◽  
pp. 486-491 ◽  
Author(s):  
Elisabeth Elass-Rochard ◽  
Dominique Legrand ◽  
Valerie Salmon ◽  
Anca Roseanu ◽  
Mihaela Trif ◽  
...  
Keyword(s):  
Escherichia Coli ◽  
Binding Sites ◽  
Lipid A ◽  
Binding Protein ◽  
Amino Acid Residues ◽  
Lipopolysaccharide Binding Protein ◽  
Maximal Inhibition ◽  
Acute Phase Serum ◽  
Lipopolysaccharide Binding ◽  
Lps Binding

ABSTRACT Human lactoferrin (hLf), a glycoprotein released from neutrophil granules during inflammation, and the lipopolysaccharide (LPS)-binding protein (LBP), an acute-phase serum protein, are known to bind to the lipid A of LPS. The LPS-binding sites are located in the N-terminal regions of both proteins, at amino acid residues 28 to 34 of hLf and 91 to 108 of LBP. Both of these proteins modulate endotoxin activities, but they possess biologically antagonistic properties. In this study, we have investigated the competition between hLf and recombinant human LBP (rhLBP) for the binding of Escherichia coli 055:B5 LPS to the differentiated monocytic THP-1 cell line. Our studies revealed that hLf prevented the rhLBP-mediated binding of LPS to the CD14 receptor on cells. Maximal inhibition of LPS-cell interactions by hLf was raised when both hLf and rhLBP were simultaneously added to LPS or when hLf and LPS were mixed with cells 30 min prior to the incubation with rhLBP. However, when hLf was added 30 min after the interaction of rhLBP with LPS, the binding of the rhLPS-LBP complex to CD14 could not be reversed. These observations indicate that hLf competes with rhLBP for the LPS binding and therefore interferes with the interaction of LPS with CD14. Furthermore, experiments involving competitive binding of the rhLBP-LPS complex to cells with two recombinant mutated hLfs show that in addition to residues 28 to 34, another basic cluster which contains residues 1 to 5 of hLf competes for the binding to LPS. Basic sequences homologous to residues 28 to 34 of hLf were evidenced on LPS-binding proteins such as LBP, bactericidal/permeability-increasing protein, and Limulus anti-LPS factor.

scholarly journals Higher Lipopolysaccharide Binding Protein and Chemerin Concentrations Were Associated with Metabolic Syndrome Features in Pediatric Subjects with Abdominal Obesity during a Lifestyle Intervention

Nutrients ◽  
2021 ◽  
Vol 13 (2) ◽  
pp. 289
Author(s):  
Amelia Marti ◽  
Isabel Martínez ◽  
Ana Ojeda-Rodríguez ◽  
María Cristina Azcona-Sanjulian
Keyword(s):  
Metabolic Syndrome ◽  
Children And Adolescents ◽  
Lifestyle Intervention ◽  
Abdominal Obesity ◽  
Plasma Levels ◽  
Intervention Program ◽  
Binding Protein ◽  
Controlled Trial ◽  
Lipopolysaccharide Binding Protein ◽  
Lipopolysaccharide Binding

Background: Elevated circulating plasma levels of both lipopolysaccharide-binding protein (LBP) and chemerin are reported in patients with obesity, but few studies are available on lifestyle intervention programs. We investigated the association of both LBP and chemerin plasma levels with metabolic syndrome (MetS) outcomes in a lifestyle intervention in children and adolescents with abdominal obesity Methods: Twenty-nine patients enrolled in a randomized controlled trial were selected. The lifestyle intervention with a 2-month intensive phase and a subsequent 10-month follow-up consisted of a moderate calorie-restricted diet, recommendations to increase physical activity levels, and nutritional education. Results: Weight loss was accompanied by a significant reduction in MetS prevalence (−43%; p = 0.009). Chemerin (p = 0.029) and LBP (p = 0.033) plasma levels were significantly reduced at 2 months and 12 months, respectively. At the end of intervention, MetS components were associated with both LBP (p = 0.017) and chemerin (p < 0.001) plasma levels. Conclusions: We describe for the first time a reduction in both LBP and chemerin plasma levels and its association with MetS risk factors after a lifestyle intervention program in children and adolescents with abdominal obesity. Therefore, LBP and chemerin plasma levels could be used as biomarkers for the progression of cardiovascular risk in pediatric populations.

scholarly journals Microbiome-Metabolomics Reveals Prebiotic Benefits of Fucoidan Supplementation in Mice

2021 ◽  
Vol 9 (5) ◽  
pp. 505
Author(s):  
Jingyi Yuan ◽  
Song Qin ◽  
Wenjun Li ◽  
Yubing Zhang ◽  
Yuting Wang ◽  
...  
Keyword(s):  
Lipid Metabolism ◽  
Triglyceride Level ◽  
Lactobacillus Reuteri ◽  
Binding Protein ◽  
Serum Triglyceride ◽  
Serum Triglyceride Level ◽  
Lipopolysaccharide Binding Protein ◽  
Lactobacillus Gasseri ◽  
Lactobacillus Animalis ◽  
Lipopolysaccharide Binding

Fucoidan is a kind of polysaccharide with antitumor and antioxidant properties, which is mainly isolated from brown algae. Although there are many reports about the prebiotic effects of polysaccharides on hosts, there are few reports about the effects of fucoidan on blood biochemical indexes, intestinal microbiome, and metabolic function on healthy hosts. We applied 16S rRNA gene amplicon sequencing and LC-MS/MS metabolomics to evaluate the changes in the gut microbiome and metabolite profiles of fucoidan treatment in mice over 10 weeks. Fucoidan treatment modulated lipid metabolism, including significantly decreasing serum triglyceride level in healthy mice. Fucoidan also significantly inhibited serum lipopolysaccharide-binding protein (LBP) concentration, a biomarker of endotoxemia. Correlation analysis further showed that Lactobacillus animalis populations that were enriched by fucoidan demonstrated significantly negative correlations with serum triglyceride level. The abundance of Lactobacillus gasseri and Lactobacillus reuteri, increased by fucoidan supplementation, demonstrated significantly negative correlation with lipopolysaccharide-binding protein levels. Lactobacillus gasseri also demonstrated significantly positive correlations with three tryptophan-related metabolites, including indoleacrylic acid, 3-indoleacrylic acid, and 5-hydroxytryptamine, which were all increased by fucoidan administration. Combined with the previous evidence, the results indicate that fucoidan exerts prebiotic effects, such as lipid metabolism suppression and metabolic endotoxemia suppression, by modulating the abundance of gut microbiota, such as Lactobacillus animalis, Lactobacillus gasseri, and Lactobacillus reuteri, as well as microbiota-dependent metabolites, such as tryptophan-related metabolites.

Gene variants of the bactericidal/permeability increasing protein and lipopolysaccharide binding protein in sepsis patients

2001 ◽  
Vol 29 (3) ◽  
pp. 557-561 ◽  
Author(s):  
Jaroslav A. Hubacek ◽  
Frank Stüber ◽  
Dieter Fröhlich ◽  
Malte Book ◽  
Silke Wetegrove ◽  
...  
Keyword(s):  
Binding Protein ◽  
Gene Variants ◽  
Lipopolysaccharide Binding Protein ◽  
Lipopolysaccharide Binding

Lipopolysaccharide-Binding Protein Is an Early Biomarker of Cardiac Function After Radiation Therapy for Breast Cancer

2019 ◽  
Vol 104 (5) ◽  
pp. 1074-1083 ◽  
Author(s):  
Justyna Chalubinska-Fendler ◽  
Lukasz Graczyk ◽  
Grzegorz Piotrowski ◽  
Krystyna Wyka ◽  
Zuzanna Nowicka ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Radiation Therapy ◽  
Cardiac Function ◽  
Binding Protein ◽  
Lipopolysaccharide Binding Protein ◽  
Early Biomarker ◽  
Lipopolysaccharide Binding

The Kinetic Expression of Lipopolysaccharide-Binding Protein and CD14 Gene in Obstructive Jaundice

2014 ◽  
Vol 28 (1) ◽  
pp. 18-23 ◽  
Author(s):  
Ching-Hua Tsai ◽  
Cheng-Hsi Yeh ◽  
Shyr-Ming Sheen-Chen ◽  
Chun-Ying Huang ◽  
Yueh-Wei Liu ◽  
...  
Keyword(s):  
Obstructive Jaundice ◽  
Binding Protein ◽  
Lipopolysaccharide Binding Protein ◽  
Kinetic Expression ◽  
Cd14 Gene ◽  
Lipopolysaccharide Binding
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