Endothelial PAS Domain Protein 1 (EPAS1) Induces Adrenomedullin Gene Expression in Cardiac Myocytes: Role of EPAS1 in an Inflammatory Response in Cardiac Myocytes

2002 ◽  
Vol 34 (7) ◽  
pp. 739-748 ◽  
Author(s):  
Toru Tanaka ◽  
Hideo Akiyama ◽  
Hiroyoshi Kanai ◽  
Mahito Sato ◽  
Shinichi Takeda ◽  
...  
Keyword(s):  
Gene Expression ◽  
Inflammatory Response ◽  
Cardiac Myocytes ◽  
Pas Domain ◽  
Domain Protein

scholarly journals Intestinal DCs global gene expression dynamics affected by recombinant baker’s yeasts saccharomyces cerevisiae

2019 ◽  
Author(s):  
Baoquan Han ◽  
Tingting Zhang ◽  
Xinyi Li ◽  
Rui Zhao ◽  
Wei Ge ◽  
...  
Keyword(s):  
Gene Expression ◽  
Saccharomyces Cerevisiae ◽  
Inflammatory Response ◽  
Budding Yeast ◽  
Global Gene Expression ◽  
Transcriptome Profile ◽  
Delivery Vehicle ◽  
Gene Delivery Vehicle ◽  
Gene Expression Dynamics

Abstract Background The baker’s yeast, saccharomyces cerevisiae, has been widely used throughout our daily life in diverse aspects for thousands of years. The saccharomyces cerevisiae was found to specifically target the dendritic cells (DCs) in mammalian with a manner of antigen-receptor interaction as described previously. It is necessary to investigate the effect of the baker’s yeasts on global gene expression dynamics of intestinal DCs and explore the possibilities of using baker’s yeast as gene delivery vehicle to modulate animal’s immune functions Results with a murine oral delivery model in vivo, we confirmed the feasibility of using budding yeast as gene delivery vehicle to the intestinal DCs using the Western blots. We then examined the transcriptome profile of the mouse intestinal DCs upon yeast stimulus. The enrichment analysis of unique transcripts indicated the beneficial role of yeast in modulating the DC-mediated adaptive immunity. Compared with previous study, we also found that a large fraction of the regulated genes is coincident with the response induced by other fungus, suggesting that the budding yeast induces a similar tailored unique genetic re-programming of DCs. Another analysis of transcriptome profile indicated that expression of β-catenin gene significantly changes DCs gene expression related to inflammatory response and cell adhesion. Conclusions Here, we defined the role of budding yeast on global gene expression of intestinal DCs, and confirmed the important role of β-catenin gene on the DCs-related inflammatory response, which provides a framework for the development of mucosa yeast-based DNA vaccine.

Inhibitory PAS domain protein is a negative regulator of hypoxia-inducible gene expression

Nature ◽  
2001 ◽  
Vol 414 (6863) ◽  
pp. 550-554 ◽  
Author(s):  
Yuichi Makino ◽  
Renhai Cao ◽  
Kristian Svensson ◽  
Göran Bertilsson ◽  
Mikael Asman ◽  
...  
Keyword(s):  
Gene Expression ◽  
Negative Regulator ◽  
Pas Domain ◽  
Inducible Gene Expression ◽  
Domain Protein ◽  
Inducible Gene

scholarly journals Telomere anchoring at the nuclear periphery requires the budding yeast Sad1-UNC-84 domain protein Mps3

2007 ◽  
Vol 179 (5) ◽  
pp. 845-854 ◽  
Author(s):  
Jennifer M. Bupp ◽  
Adriana E. Martin ◽  
Elizabeth S. Stensrud ◽  
Sue L. Jaspersen
Keyword(s):  
Gene Expression ◽  
Nuclear Periphery ◽  
S Phase ◽  
Integral Membrane Proteins ◽  
Membrane Anchor ◽  
Acidic Domain ◽  
Regulator Protein ◽  
Domain Protein ◽  
Necessary And Sufficient

Positioning of telomeres at the nuclear periphery can have dramatic effects on gene expression by establishment of heritable, transcriptionally repressive subdomains. However, little is known about the integral membrane proteins that mediate telomere tethering at the nuclear envelope. Here, we find a previously unrecognized function for the Saccharomyces cerevisiae Sad1-UNC-84 domain protein Mps3 in regulating telomere positioning in mitotic cells. Our data demonstrate that the nucleoplasmic N-terminal acidic domain of Mps3 is not essential for viability. However, this acidic domain is necessary and sufficient for telomere tethering during S phase and the silencing of reporter constructs integrated at telomeres. We show that this is caused by the role of the Mps3 acidic domain in binding and localization of the silent information regulator protein Sir4 to the nuclear periphery. Thus, Mps3 functions as an integral membrane anchor for telomeres and is a novel nuclear receptor for the Sir4 pathway of telomere tethering and gene inactivation.

scholarly journals Transcriptional Up-regulation of Inhibitory PAS Domain Protein Gene Expression by Hypoxia-inducible Factor 1 (HIF-1)

2007 ◽  
Vol 282 (19) ◽  
pp. 14073-14082 ◽  
Author(s):  
Yuichi Makino ◽  
Rie Uenishi ◽  
Kensaku Okamoto ◽  
Tsubasa Isoe ◽  
Osamu Hosono ◽  
...  
Keyword(s):  
Gene Expression ◽  
Protein Gene ◽  
Hypoxia Inducible Factor ◽  
Pas Domain ◽  
Hypoxia Inducible Factor 1 ◽  
Domain Protein
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