Effects of Endothelin-1 on Ca2+Signaling in Guinea-Pig Ventricular Myocytes: Role of Protein Kinase C

1999 ◽  
Vol 31 (3) ◽  
pp. 631-643 ◽  
Author(s):  
Sun Hee Woo ◽  
Chin Ok Lee
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Guinea Pig ◽  
Ventricular Myocytes ◽  
Endothelin 1 ◽  
Kinase C

Role of protein kinase C in α1-adrenergic regulation of aNa i in guinea pig ventricular myocytes

2000 ◽  
Vol 279 (4) ◽  
pp. H1661-H1668 ◽  
Author(s):  
Su-Hyun Jo ◽  
Chung-Hyun Cho ◽  
Soo Wan Chae ◽  
Chin O. Lee
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Guinea Pig ◽  
Ventricular Myocytes ◽  
Receptor Activation ◽  
Adrenergic Regulation ◽  
Kinase C ◽  
Gf 109203X ◽  
Stimulation Of

We investigated the role of protein kinase C (PKC) in α1-adrenergic regulation of intracellular Na+activity (aNa i) in single guinea pig ventricular myocytes. aNa i and membrane potentials were measured with the Na+-sensitive indicator sodium-binding benzofuran isophthalate and conventional microelectrodes, respectively, at room temperature (24–26°C) while myocytes were stimulated at a rate of 0.25–0.3 Hz. The PKC activator 4β-phorbol 12-myristate 13-acetate (PMA) decreased aNa i in a concentration-dependent manner. PMA (100 nM) produced a maximal decrease in aNa i of 1.5 mM from 6.5 ± 0.4 to 5.0 ± 0.4 mM (means ± SE, n = 12, P< 0.01). The PMA concentration required for a half-maximal decrease in aNa i was 0.46 ± 0.13 nM ( n = 3, P < 0.01). An inactive phorbol, 4α-phorbol 12-myristate 13-acetate, did not decrease aNa i. The decrease caused by PMA could be blocked by the PKC inhibitors staurosporine and bisindolylmaleimide I (GF-109203X). Stimulation of the α1-adrenoceptor with 50 μM phenylephrine decreased aNa i from 6.1 ± 0.3 to 4.6 ± 0.3 mM ( n = 11, P< 0.01). The decrease in aNa i produced by phenylephrine was blocked by pretreatment with staurosporine, GF-109203X, or PMA. The decrease in aNa i produced by PMA was not prevented by pretreatment with tetrodotoxin but was blocked by pretreatment with strophanthidin or high extracellular K+ concentration. The results suggest that α1-adrenergic receptor activation results in a decrease in aNa i via PKC-induced stimulation of the Na+-K+ pump in cardiac myocytes.

Role of Protein Kinase C in Duodenal Mucosal Bicarbonate Secretion in the Guinea Pig

Pharmacology ◽  
1996 ◽  
Vol 53 (1) ◽  
pp. 60-65 ◽  
Author(s):  
H.S. Odes ◽  
R. Reimer ◽  
R. Muallem ◽  
M. Schwenk ◽  
W. Beil ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Guinea Pig ◽  
Bicarbonate Secretion ◽  
Kinase C

scholarly journals Muscarinic Autoinhibition and Modulatory Role of Protein Kinase C in Acetylcholine Release from the Myenteric Plexus of Guinea Pig Ileum.

1997 ◽  
Vol 74 (2) ◽  
pp. 155-163 ◽  
Author(s):  
Noriko Saitoh ◽  
Ritsuko Fujimoto ◽  
Toshiaki Ishii ◽  
Hideaki Nishio ◽  
Tadayoshi Takeuchi ◽  
...  
Keyword(s):  
Protein Kinase C ◽  
Protein Kinase ◽  
Guinea Pig ◽  
Myenteric Plexus ◽  
Acetylcholine Release ◽  
Guinea Pig Ileum ◽  
Kinase C
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