Identification and Characterization of a Novel Polycystin Family Member, Polycystin-L2, in Mouse and Human: Sequence, Expression, Alternative Splicing, and Chromosomal Localization

Genomics ◽  
2000 ◽  
Vol 64 (3) ◽  
pp. 241-251 ◽  
Author(s):  
Lei Guo ◽  
Taylor H. Schreiber ◽  
Stanislawa Weremowicz ◽  
Cynthia C. Morton ◽  
Charles Lee ◽  
...  
Keyword(s):  
Alternative Splicing ◽  
Family Member ◽  
Chromosomal Localization ◽  
Human Sequence ◽  
Identification And Characterization

Identification and characterization of mouse homologue to yeast Cdc7 protein and chromosomal localization of the cognate mouse gene Cdc7l

Chromosoma ◽  
1999 ◽  
Vol 108 (1) ◽  
pp. 26-31 ◽  
Author(s):  
Thomas Faul ◽  
Caroline Staib ◽  
Indrajit Nanda ◽  
Michael Schmid ◽  
Friedrich Grummt
Keyword(s):  
Chromosomal Localization ◽  
Mouse Gene ◽  
Mouse Homologue ◽  
Identification And Characterization

Cloning and characterization of an alternative splicing transcript of the gene coding for human cytidine deaminase

2007 ◽  
Vol 85 (1) ◽  
pp. 96-102 ◽  
Author(s):  
Bianca Cristina Garcia Lisboa ◽  
Tamara da Rocha Machado ◽  
Daniel Carvalho Pimenta ◽  
Sang Won Han
Keyword(s):  
Alternative Splicing ◽  
Dna Sequences ◽  
Genomic Sequence ◽  
Cytidine Deaminase ◽  
Alternative Form ◽  
Nih3t3 Cells ◽  
Reading Frame ◽  
Gene Coding ◽  
Identification And Characterization

Human cytidine deaminase (HCD) catalyzes the deamination of cytidine or deoxycytidine to uridine or deoxyuridine, respectively. The genomic sequence of HCD is formed by 31 kb with 4 exons and several alternative splicing signals, but an alternative form of HCD has yet to be reported. Here we describe the cloning and characterization of a small form of HCD, HSCD, and it is likely to be a product of alternative splicing of HCD. The alignment of DNA sequences shows that the HSCD matches HCD in 2 parts, except for a deletion of 170 bp. Based on the HCD genome organization, exons 1 and 4 should be joined and all sequences of introns and exons 2 and 3 should be deleted by splicing. This alternative splicing shifted the translation of the reading frame from the point of splicing. The estimated molecular mass is 9.8 kDa, and this value was confirmed by Western blot and mass spectroscopy after expressing the gene fused with glutathionine-S-transferase in the pGEX vector. The deletion and shift of the reading frame caused a loss of HCD activity, which was confirmed by enzyme assay and also with NIH3T3 cells modified to express HSCD and challenged against cytosine arabinoside. In this work we describe the identification and characterization of HSCD, which is the product of alternative splicing of the HCD gene.

scholarly journals Identification and characterization of alternative splicing in parasitic nematode transcriptomes

2014 ◽  
Vol 7 (1) ◽  
pp. 151 ◽  
Author(s):  
Sahar Abubucker ◽  
Samantha N McNulty ◽  
Bruce A Rosa ◽  
Makedonka Mitreva
Keyword(s):  
Alternative Splicing ◽  
Parasitic Nematode ◽  
Identification And Characterization
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