Trypanosoma vivax: Characterization of the Spliced-Leader Gene of a Brazilian Stock and Species-Specific Detection by PCR Amplification of an Intergenic Spacer Sequence

2001 ◽  
Vol 99 (1) ◽  
pp. 37-48 ◽  
Author(s):  
Rogéria M Ventura ◽  
Fernando Paiva ◽  
Roberto A.M.S Silva ◽  
Gentilda F Takeda ◽  
Gregory A Buck ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Intergenic Spacer ◽  
Trypanosoma Vivax ◽  
Specific Detection ◽  
Spliced Leader ◽  
Species Specific

scholarly journals Characterization of a Trypanosoma cruzi genomic fragment complementary to several species-specific mRNAs but different from the spliced leader sequence

1989 ◽  
Vol 84 (2) ◽  
pp. 143-149 ◽  
Author(s):  
Paulo Cesar Cotrim ◽  
Leda Maria Cummings ◽  
José Franco da Silveira
Keyword(s):  
Trypanosoma Cruzi ◽  
Leader Sequence ◽  
Spliced Leader ◽  
Dna And Rna ◽  
Specific Mrnas ◽  
Species Specific ◽  
Coding Capacity ◽  
Specific Sequences ◽  
Trypanosoma Species

We have isolated a clone of Trypanosoma cruzi genimic DNA, lambda 3b2-5, which contains sequences that are reiterated in the genome. Northtern blot analysis showed that clone 3b2-5 hybridizes to 1,200-5,000 bases different mRNA species. The number of mRNAs species hybridized to clone 3b2-5 exceeds its coding capacity showing that this clone carries sequences that are common to several mRNAs species and conserved in the poly A(+) RNA. These sequences are not homologous to the T. cruzi spliced leader sequence, since clone 3b2-5 hybridize to a synthetic 20 nucleotice complementary to the spliced leader sequence. Clone 3b2-5 does not hybridize to DNA and RNA from several genera of Trypanosomatidae and other Trypanosoma species indicating that it carries T. cruzi species-specific sequences.

Direct and Highly Species-Specific Detection of Pork Meat and Fat in Meat Products by PCR Amplification of Mitochondrial DNA

2000 ◽  
Vol 48 (7) ◽  
pp. 2829-2832 ◽  
Author(s):  
J. F. Montiel-Sosa ◽  
E. Ruiz-Pesini ◽  
J. Montoya ◽  
P. Roncalés ◽  
M. J. López-Pérez ◽  
...  
Keyword(s):  
Mitochondrial Dna ◽  
Meat Products ◽  
Pcr Amplification ◽  
Pork Meat ◽  
Specific Detection ◽  
Species Specific

scholarly journals Characterization of Colletotrichum acutatum Causing Anthracnose of Anemone (Anemone coronaria L.)

2000 ◽  
Vol 66 (12) ◽  
pp. 5267-5272 ◽  
Author(s):  
Stanley Freeman ◽  
Ezra Shabi ◽  
Talma Katan
Keyword(s):  
Western Europe ◽  
Pcr Amplification ◽  
Vegetative Compatibility ◽  
Colletotrichum Acutatum ◽  
Vegetative Compatibility Groups ◽  
The Third ◽  
Artificial Inoculations ◽  
Species Specific ◽  
Arbitrarily Primed Pcr

ABSTRACT Anthracnose, or leaf-curl disease of anemone, caused byColletotrichum sp., has been reported to occur in Australia, western Europe, and Japan. Symptoms include tissue necrosis, corm rot, leaf crinkles, and characteristic spiral twisting of floral peduncles. Three epidemics of the disease have been recorded in Israel: in 1978, in 1990 to 1993, and in 1996 to 1998. We characterized 92Colletotrichum isolates associated with anthracnose of anemone (Anemone coronaria L.) for vegetative compatibility (72 isolates) and for molecular genotype (92 isolates) and virulence (4 isolates). Eighty-six of the isolates represented the three epidemics in Israel, one isolate was from Australia, and five isolates originated from western Europe. We divided these isolates into three vegetative-compatibility groups (VCGs). One VCG (ANE-A) included all 10 isolates from the first and second epidemics, and 13 of 62 examined isolates from the third epidemic in Israel, along with the isolate from Australia and 4 of 5 isolates from Europe. Another VCG (ANE-F) included most of the examined isolates (49 of the 62) from the third epidemic, as well as Colletotrichum acutatum from strawberry, in Israel. Based on PCR amplification with species-specific primers, all of the anemone isolates were identified as C. acutatum. Anemone and strawberry isolates of the two VCGs were genotypically similar and indistinguishable when compared by arbitrarily primed PCR of genomic DNA. Only isolate NL-12 from The Netherlands, confirmed as C. acutatum but not compatible with either VCG, had a distinct genotype; this isolate represents a third VCG of C. acutatum. Isolates from anemone and strawberry could infect both plant species in artificial inoculations. VCG ANE-F was recovered from natural infections of both anemone and strawberry, but VCG ANE-A was recovered only from anemone. This study of C. acutatum from anemone illustrates the potential of VCG analysis to reveal distinct subspecific groups within a pathogen population which appears to be genotypically homogeneous by molecular assays.

scholarly journals Characterization of the Race Structure of Leptosphaeria maculans Causing Blackleg of Winter Canola in Oklahoma and Kansas

Plant Disease ◽  
2019 ◽  
Vol 103 (9) ◽  
pp. 2353-2358 ◽  
Author(s):  
Claudia Diaz ◽  
Felipe Cevallos ◽  
John Damicone
Keyword(s):  
Cultural Practices ◽  
Quantitative Resistance ◽  
Genetic Resistance ◽  
Leptosphaeria Maculans ◽  
Pcr Amplification ◽  
Avirulence Genes ◽  
Winter Canola ◽  
Differential Cultivars ◽  
Species Specific

Blackleg, caused by the fungus Leptosphaeria maculans, is a widespread disease of winter canola (Brassica napus) in Oklahoma and Kansas. Deployment of genetic resistance is the primary strategy for managing blackleg. Resistance genes (Rlm) in canola interact with avirulence genes in the fungus (AvrLm) in a gene-for-gene manner. Little is known about the diversity and frequency of avirulence genes and the race structure in the region. Isolates of Leptosphaeria spp. were collected from diseased leaves in nine counties in Oklahoma and one county in Kansas from 2009 to 2013. Based on pathogenicity and PCR amplification of mating type and species-specific internal transcribed spacer loci, most isolates (n = 90) were L. maculans. The presence of avirulence genes was evaluated using phenotypic interactions on cotyledons of differential cultivars with Rlm1, Rlm2, Rlm3, and Rlm4 and amplification of AvrLm1, AvrLm4-7, and AvrLm6 by PCR. The avirulence alleles AvrLm6 and AvrLm7 were present in the entire L. maculans population. AvrLm1 was found in 34% of the population, AvrLm2 in 4%, and AvrLm4 in only 1%. A total of five races, defined as combinations of avirulence alleles, were identified that included AvrLm1-2-6-7, AvrLm2-6-7, AvrLm4-6-7, AvrLm1-6-7, and AvrLm6-7. Races virulent on the most Rlm genes, AvrLm1-6-7 at 32% and AvrLm6-7 at 62%, were predominant. Defining the avirulence allele frequency and race structure of L. maculans should be useful for the identification and development of resistant cultivars and hybrids for blackleg management in the region. The results suggest that Rlm6 and Rlm7 would be effective, although their deployment should be integrated with quantitative resistance and cultural practices, such as crop rotation, that limit selection pressure on Rlm genes.

scholarly journals Identification of Nile Perch (Lates niloticus), Grouper (Epinephelus guaza), and Wreck Fish (Polyprion americanus) Fillets by PCR Amplification of the 5S rDNA Gene

2001 ◽  
Vol 84 (3) ◽  
pp. 777-781 ◽  
Author(s):  
Luis Asensio ◽  
Isabel González ◽  
Alicia Fernández ◽  
Ana Céspedes ◽  
Miguel A Rodríguez ◽  
...  
Keyword(s):  
Pcr Amplification ◽  
Intergenic Spacer ◽  
5S Rdna ◽  
Nile Perch ◽  
Intergenic Spacer Region ◽  
Rdna Gene ◽  
Lates Niloticus ◽  
Polymerase Chain ◽  
Species Specific ◽  
Polyprion Americanus

Abstract Nile perch (Lates niloticus), grouper (Epinephelus guaza), and wreck fish (Polyprion americanus) were differentiated by polymerase chain reaction (PCR) amplification of the nuclear 5S rDNA gene. The design of 3 species-specific primers complementary to the nontranscribed intergenic spacer region from the 5S rDNA molecule allowed amplification of clearly distinguishable gene fragments in each fish species. This approach is rapid and reliable and offers the potential to detect fraudulent or unintentional mislabeling of these species in routine seafood authentication analysis.

Species-specific Detection of Campylobacters Important in Veterinary Medicine by PCR Amplification of 23S rDNA Areas

1995 ◽  
Vol 17 (4) ◽  
pp. 563-568 ◽  
Author(s):  
Katrin Bastyns ◽  
Sabine Chapelle ◽  
Peter Vandamme ◽  
Herman Goossens ◽  
Rupert De Wachter
Keyword(s):  
Veterinary Medicine ◽  
Pcr Amplification ◽  
Specific Detection ◽  
Species Specific
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